Development of a colloidal gold immunochromatographic strip for the rapid detection of antibodies against Fasciola gigantica in buffalo

Wang, Jinhui; He, Kangxin; Wu, Zhengjiao; Jin, Weikun; Wu, Wende; Guo, Yanfeng; Zhang, Weiyu; Di, Wenda

doi:10.3389/fvets.2022.1004932

Cited by 4 publications

(5 citation statements)

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“…Moreover, ICS testing is considered to be a tool to detect antigens [ 23 , 24 ] or antibodies [ 25 ] in medical field, comprising a test strip for the detection of an antigen or antibody in sample solutions, such as a secretion, serum, stool samples, and urine. The ICS test strip has been extensively applied for the diagnosis of viruses or parasites, such as the laryngotracheitis virus [ 26 ], the SARS-CoV-2 virus [ 27 ], Schistosoma japonicum [ 16 , 28 ], Angiostrongylus cantonensis [ 29 ], Toxoplasma gondii [ 30 , 31 ], Leishmania donovani [ 32 ], and F. gigantica [ 33 ]. However, no ICS tests for detecting fasciolosis using rFgCathL1H antigens have been developed to detect anti-FgCathL1H antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…In previous research, Wang et al applied F. gigantica excretory-secretory products (FgESP) to detect antibodies in mice serum present at the T-line. Goat anti-mouse secondary antibodies coated the C-line [ 33 ]. However, testing infected cattle serum requires the changing of the protein at the C-line to anti-buffalo secondary antibody 2G7 [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Goat anti-mouse secondary antibodies coated the C-line [ 33 ]. However, testing infected cattle serum requires the changing of the protein at the C-line to anti-buffalo secondary antibody 2G7 [ 33 ]. In our study, we purified rabbit anti-rFgCahL1H, which is able to attach itself to both the antibodies of mice and cattle.…”

Section: Discussionmentioning

confidence: 99%

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Fasciola gigantica Cathepsin L1H: High Sensitivity and Specificity of Immunochromatographic Strip Test for Antibody Detection

2023

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Fasciolosis is a zoonotic disease caused by Fasciola gigantica or F. hepatica infections, which are frequently occurring parasites in animals and humans. The present gold-standard diagnostic technique involves finding parasite eggs through microscopy. However, this method is also restricted due to low specificity and low sensitivity. An alternative to coprological diagnosis is the immunochromatographic strip (ICS) test, which is rapid, simple, convenient, and cost-effective, with high sensitivity and high specificity. Cathepsin L1H (CathL1H) is a cysteine protease secreted by F. gigantica, which is found in high amounts in newly excysted juvenile (NEJ) and juvenile stages. Cathepsin L1H plays an important role in both the immune response to invading pathogens and in the ability of some pathogens to evade the host immune system. The present study aims to develop an ICS test and detect antibodies against CathL1H in mice and cattle serum using the recombinant F. gigantica Cathepsin L1H (rFgCathL1H) and rabbit anti-rFgCathL1H antibody. The F. gigantica-infected serum and non-infected serum of mice and cattle were tested using the ICS test. Moreover, the strip results were confirmed with an indirect enzyme-linked immunosorbent assay (indirect ELISA). The relative sensitivity, specificity, and accuracy of the ICS strip were 97.5, 99.99, and 99.00%, respectively. Therefore, these data suggest that the ICS method could be used to detect F. gigantica antibodies to highly enhance throughput, reduce costs, and determine the best alternative on-site method.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Fasciola gigantica Cathepsin L1H: High Sensitivity and Specificity of Immunochromatographic Strip Test for Antibody Detection

2023

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“…F. gigantica eggs were also detected in the faeces between 12 and 14 wpi. In addition, autopsies at 16 wpi found livers from the infection group to show obvious gross pathological lesions, and adult flukes were detected and the mean numbers of flukes recovered were 55.5 ± 14.1 (22.2 ± 5.6 of infection dose), indicating established infections (Wang et al, 2022a). All buffaloes in the non-infection group had negative indirect FgESP-based ELISA findings.…”

Section: Fasciola Gigantica Infection Confirmationmentioning

confidence: 97%

Proteomic analysis of Fasciola gigantica excretory and secretory products (FgESPs) co-immunoprecipitated using a time course of infected buffalo sera

et al. 2022

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IntroductionWidespread Fasciola gigantica infection in buffaloes has caused great economic losses in buffalo farming. Studies on F. gigantica excretory and secretory products (FgESP) have highlighted their importance in F. gigantica parasitism and their potential in vaccine development. Identifying FgESP components involved in F. gigantica-buffalo interactions during different periods is important for developing effective strategies against fasciolosis.MethodsBuffaloes were assigned to non-infection (n = 3, as control group) and infection (n = 3) groups. The infection group was orally administrated 250 metacercariae. Sera were collected at 3, 10, and 16 weeks post-infection (wpi) for the non-infection group and at 0 (pre-infection), 1, 3, 6, 8, 10, 13, and 16 wpi for the infection group. FgESP components interacting with sera from the non-infection and infection groups assay were pulled down by co-IP and identified using LC–MS/MS. Interacting FgESP components in infection group were subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway and gene ontology (GO) functional annotation to infer their potential functions.Results and discussionProteins of FgESP components identified in the non-infection group at 3, 10, and 16 wpi accounted for 80.5%, 84.3%, and 82.1% of all proteins identified in these three time points, respectively, indicating surroundings did not affect buffalo immune response during maintenance. Four hundred and ninety proteins were identified in the infection group, of which 87 were consistently identified at 7 time points. Following GO analysis showed that most of these 87 proteins were in biological processes, while KEGG analysis showed they mainly functioned in metabolism and cellular processing, some of which were thought to functions throughout the infection process. The numbers of specific interactors identified for each week were 1 (n = 12), 3 (n = 5), 6 (n = 8), 8 (n = 15), 10 (n = 23), 13 (n = 22), and 16 (n = 14) wpi, some of which were thought to functions in specific infection process. This study screened the antigenic targets in FgESP during a dense time course over a long period. These findings may enhance the understanding of molecular F. gigantica-buffalo interactions and help identify new potential vaccine and drug target candidates.

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“…As a powerful POC tool, immumochromatographic assay (ICA) has been widely developed with a particular application in epidemiological surveys in the field. Recently, a number of colloidal gold immunochromatographic assay (GICA) strips have been established and evaluated for the rapid diagnosis of parasitic diseases, such as schistosomiasis ( 28 – 31 ), toxoplasmosis ( 32 ), fascioliasis ( 33 ) and opisthorchiasis ( 34 ), as an alternative screening tool.…”

Section: Introductionmentioning

confidence: 99%

Development and assessment of a novel gold immunochromatographic assay for the diagnosis of schistosomiasis japonica

McManus

Gordon

et al. 2023

Front. Immunol.

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BackgroundThe neglected zoonosis, schistosomiasis japonica, remains a major public health problem in the Philippines. The current study aims to develop a novel gold immunochromatographic assay (GICA) and evaluate its performance in the detection of Schistosoma japonicum infection.MethodsA GICA strip incorporating a S. japonicum saposin protein, SjSAP4 was developed. For each GICA strip test, diluted serum sample (50 µl) was loaded and strips were scanned after 10 min to convert the results into images. ImageJ was used to calculate an R value, which was defined as the signal intensity of the test line divided by the signal intensity of the control line within the cassette. After determination of optimal serum dilution and diluent, the GICA assay was evaluated with sera collected from non-endemic controls (n = 20) and individuals living in schistosomiasis-endemic areas of the Philippines (n = 60), including 40 Kato Katz (KK)-positive participants and 20 subjects confirmed as KK-negative and faecal droplet digital PCR assay (F_ddPCR)-negative at a dilution of 1:20. An ELISA assay evaluating IgG levels against SjSAP4 was also performed on the same panel of sera.ResultsPhosphate-buffered saline (PBS) and 0.9% NaCl were determined as optimal dilution buffer for the GICA assay. The strips tested with serial dilutions of a pooled serum sample from KK-positive individuals (n = 3) suggested that a relatively wide range of dilutions (from 1:10 to 1:320) can be applied for the test. Using the non-endemic donors as controls, the GICA strip showed a sensitivity of 95.0% and absolute specificity; while using the KK-negative and F_ddPCR-negative subjects as controls, the immunochromatographic assay had a sensitivity of 85.0% and a specificity of 80.0%. The SjSAP4-incorperated GICA displayed a high concordance with the SjSAP4-ELISA assay.ConclusionsThe developed GICA assay exhibited a similar diagnostic performance with that of the SjSAP4-ELISA assay, yet the former can be performed by local personnel with minimal training with no requirement for specialised equipment. The GICA assay established here represents a rapid, easy-to-use, accurate and field-friendly diagnostic tool for the on-site surveillance/screening of S. japonicum infection.

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Development of a colloidal gold immunochromatographic strip for the rapid detection of antibodies against Fasciola gigantica in buffalo

Abstract: Development of a colloidal gold immunochromatographic strip for the rapid detection of antibodies against Fasciola gigantica in bu alo.

Cited by 4 publications

References 20 publications

Fasciola gigantica Cathepsin L1H: High Sensitivity and Specificity of Immunochromatographic Strip Test for Antibody Detection

Fasciola gigantica Cathepsin L1H: High Sensitivity and Specificity of Immunochromatographic Strip Test for Antibody Detection

Proteomic analysis of Fasciola gigantica excretory and secretory products (FgESPs) co-immunoprecipitated using a time course of infected buffalo sera

Development and assessment of a novel gold immunochromatographic assay for the diagnosis of schistosomiasis japonica

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