Cloning and expression of the carbaryl hydrolase gene mcbA and the identification of a key amino acid necessary for carbaryl hydrolysis

Conserved Metabolic and Evolutionary Themes in Microbial Degradation of Carbamate Pesticides

“…McbA from strain XWY-1 was purified and characterised. This protein harboured three histidine residues (His467, His477 and His504) in the predicted polymerase/histidinol phosphatase-like domain, that were crucial for hydrolysis ( Zhu et al, 2018 ). The 1-naphthol generated undergoes hydroxylation by 1-naphthol 2-hydroxylase (1NH) to yield 1,2-dihydroxynaphthalene.…”

Mineralization of the herbicide swep by a two-strain consortium and characterization of a new amidase for hydrolyzing swep

“…Over 1000 types of carbamate pesticides have been developed, and their use has exceeded that of organophosphorus pesticides, with the sales of carbamate herbicides being only second to pyrethroid pesticides [1]. However, the extensive use of carbamate herbicides leads to the presence of carbamate herbicide residues in the environment, threating ecosystems and human health [2][3][4]. Since the 1970s, the use of carbamate herbicides has increased every year.…”

Isolation of bacteria that degrade carbamate insecticides and characterization of the functional and ecological role of bacterial genes involved in their hydrolysis in soil

“…Enrichment cultures from the adapted soil resulted in the isolation of four bacterial strains, which were all identified, based on MLSA, as Pseudomonas. Earlier studies have reported the isolation of pseudomonads rapidly degrading a range of pesticides including organophosphates , carbamates (Bano and Musarrat, 2006;Zhu et al 2019), and pyrethroids ). Our study constitutes only the second report regarding the isolation of oxamyldegrading bacteria.…”

“…Four oxamyl-degrading bacterial strains were isolated from the adapted soil via enrichment cultures and identified, based on MLSA, as Pseudomonas. Bacteria belonging to the genus Pseudomonas are metabolic champions able to degrade a diverse range of compounds including pesticides like organophosphates , carbamates Zhu et al 2019) and pyrethroids ). These results constitute only the second report regarding the isolation of oxamyl-degrading bacteria.…”

“…In contrast to the two previous reports Öztürk et al 2016), more recent studies by demonstrated that rhizobial CehA was able to recognize carbofuran as a substrate, albeit with low catabolic efficiency.In 2006 Hashimoto et al reported the isolation of an amidase-encoding gene cahA from an Arthrobacter strain, which was able to degrade carbaryl. CahA hydrolyzed also the carbamate insecticides xylycarb, metolcarb and XMC but was less reactive with fenobucarb, isoprocarb and propoxur(Hayatsu 2001) Another carbamate hydrolase encoding gene mcbA has been isolated from two carbaryl-degrading Pseudomonas strains and reported as a carbaryl esterase(Zhu et al 2018;). The amino acid sequence of McbA of the two strains differ in one amino acid and displayed only 24% identity with CehA from Rhizobium sp.…”