Cloning and expression of the flavin reductase LuxG from Photobacterium leiognathi YL and its improvement for NADH detection

Xuan, Guanhua; Xiao, Qilin; Wang, Jingxue; Lin, Hong

doi:10.1039/c9pp00435a

Cited by 3 publications

(1 citation statement)

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“…[ 69 ] Later on, the detection limit of this system could be enhanced with a detection range of 0.1–1 n m when the purified recombinant LuxG and Lux enzymes were used instead. [ 70 ] LuxG, a flavin reductase found in the same operon as Lux, prefers to use FMN more than other flavins, while Fre has more preference toward riboflavin. [ 71 ] Lux has a greater advantage over Luc for using simple and cheap substrates and does not require a specialty compound such as proluciferin.…”

Section: Nad(p)h Detection As An Indicator For Energy Level and Cell ...mentioning

confidence: 99%

Detection of cellular metabolites by redox enzymatic cascades

Tinikul

Trisrivirat

Chinantuya

et al. 2022

Biotechnology Journal

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Detection of cellular metabolites that are disease biomarkers is important for human healthcare monitoring and assessing prognosis and therapeutic response. Accurate and rapid detection of microbial metabolites and pathway intermediates is also crucial for the process optimization required for development of bioconversion methods using metabolically engineered cells. Various redox enzymes can generate electrons that can be employed in enzyme‐based biosensors and in the detection of cellular metabolites. These reactions can directly transform target compounds into various readout signals. By incorporating engineered enzymes into enzymatic cascades, the readout signals can be improved in terms of accuracy and sensitivity. This review critically discusses selected redox enzymatic and chemoenzymatic cascades currently employed for detection of human‐ and microbe‐related cellular metabolites including, amino acids, d‐glucose, inorganic ions (pyrophosphate, phosphate, and sulfate), nitro‐ and halogenated phenols, NAD(P)H, fatty acids, fatty aldehyde, alkane, short chain acids, and cellular metabolites.

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Section: Nad(p)h Detection As An Indicator For Energy Level and Cell ...mentioning

confidence: 99%