Cloning and expression of the sorbitol dehydrogenase gene during embryonic development and temperature stress in Artemia sinica

Wang, Ting; Hou, Ming‐Feng; Zhao, Ning; Chen, Yifei; Lv, Ying; Li, Zengrong; Zhang, Rui; Xin, Wenting; Zou, Xiangyang; Lin, Hui

doi:10.1016/j.gene.2013.03.077

Cited by 14 publications

(8 citation statements)

References 29 publications

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“…Compared with the diapause egg, non-diapause eggs displayed higher sorbitol dehydrogenase activity during early embryonic development [24]. Temperature stress and HCl treatment can increase the expression of sorbitol dehydrogenase [13,25]. In the present study, two sorbitol dehydrogenases (BGIBMGA012399 and BGIBMGA012400) were significantly upregulated at 12 h after the O 2 treatment (Table S8), but not after the HCl treatment, compared with the control.…”

Section: Discussionmentioning

confidence: 48%

Transcriptional Response of Silkworm (Bombyx mori) Eggs to O2 or HCl Treatment

Gong

Tian

Zhou

et al. 2016

IJMS

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Diapause is a common biological phenomenon that occurs in many organisms, including fish, insects, and nematodes. In the silkworm (Bombyx mori), diapause generally occurs in the egg stage. Treatment with O2, HCl, or other compounds can prevent egg diapause. Here, we characterized the transcriptomic responses of newly laid eggs treated with O2 or HCl. Digital gene expression analysis showed that 610 genes in O2-treated eggs and 656 in HCl-treated eggs were differentially expressed. Of these, 343 genes were differentially expressed in both treatments. In addition to trehalases, sorbic acid dehydrogenases, and some enzymes involved in the carbohydrate metabolism, we also identified heat shock proteins, cytochrome P450, and GADD45, which are related to stress tolerance. Gene ontology enrichment analysis showed differentially expressed genes in O2-treated eggs were involved in oxidoreductase activity as well as in binding, catalytic, and metabolic processes. The Kyoto Encyclopedia of Genes and Genomes analysis showed that the pathways for ribosome biogenesis, spliceosome, and circadian rhythm were significantly enriched in HCl-treated eggs. The reliability of the data was confirmed by qRT-PCR analysis. Our results improved the understanding of the mechanism of diapause blocking in silkworm eggs treated with O2 or HCl and identified novel molecular targets for future studies.

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Section: Discussionmentioning

confidence: 48%

Transcriptional Response of Silkworm (Bombyx mori) Eggs to O2 or HCl Treatment

Gong

Tian

Zhou

et al. 2016

IJMS

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“…A. sinica has a relatively low classification status, and few differences were found between As-RBBP4 and homologs in other species. Other genes of A. sinica also showed similar characteristics as it is classified between higher vertebrates and low invertebrates Jiang et al, 2007;Li et al, 2013;Wang et al, 2013;Li et al, 2014 ).…”

Section: Discussionmentioning

confidence: 66%

Cloning and expression of retinoblastoma-binding protein 4 gene in embryo diapause termination and in response to salinity stress from brine shrimp Artemia sinica

Wang

Yao

Liang

et al. 2016

Gene

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“…The holoenzyme of SDH is a tetramer with MW of 38000 Da and 348 amino acid proteins expression pattern were investigated during the different stages of embryonic development (Wang, et al, 2013). Similarly in the plasma of Rat SDH is tetramer with four identical monomers (Johansson et al, 2001).…”

Section: Resultsmentioning

confidence: 99%

Purification of sorbitol dehydrogenase from diapause eggs of Dysdercus cingulatus (fabricus 1775)

Shahjahan

Martin

2017

IJARe

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Sorbitol Dehydrogenase (SDH) is important enzymes responsible for the protection of eggs from harsh condition as it convert fructose to sorbitol which serves as polyol and stabilize the soluble proteins and lipid bilayer. The enzyme SDH (dIdiol NAD oxidoreductase EC 1.1.1.14) is purified from the eggs of red cotton bug, Dysdercus cingulatus exposed to low temperature. The SDH from eggs were purified by using different protein purification technique viz, ion exchange chromatography, gel filtration and SDS-PAGE. The ion exchange chromatography showed 0.185U total activity, with a purification fold of 1.48 and yield 3.36%. Gel filtration chromatography showed an increase in purification of SDH activity by 2.73 with 0.095 U total activity and yield of 1.73%. SDS-PAGE revealed that SDH molecular weight of 38 KDa. The Km value of Fructose: NADH is 0.4:0.01 and the Vmax was 0.6 and 0.03. Thus low temperature increases the activity of SDH which convert fructose to sorbitol which protects the cell during diapause condition.

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Cloning and expression of the sorbitol dehydrogenase gene during embryonic development and temperature stress in Artemia sinica

Cited by 14 publications

References 29 publications

Transcriptional Response of Silkworm (Bombyx mori) Eggs to O2 or HCl Treatment

Transcriptional Response of Silkworm (Bombyx mori) Eggs to O2 or HCl Treatment

Cloning and expression of retinoblastoma-binding protein 4 gene in embryo diapause termination and in response to salinity stress from brine shrimp Artemia sinica

Purification of sorbitol dehydrogenase from diapause eggs of Dysdercus cingulatus (fabricus 1775)

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