Cloning and expression of the gene for periplasmic poly(vinyl alcohol) dehydrogenase from Sphingomonas sp. strain 113P3, a novel-type quinohaemoprotein alcohol dehydrogenase

Hirota-Mamoto, Rie; Nagai, Ryoko; Tachibana, Sanro; Yasuda, Masaaki; Tani, Akio; Kimbara, Kazuhide; Kawai, Fusako

doi:10.1099/mic.0.28848-0

Cited by 33 publications

(15 citation statements)

References 21 publications

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“…Cytochrome c has been suggested to be a natural electron acceptor for PVA-DH in vivo. 49) PVA-DH is a member of the type-II quinohemoprotein alcohol dehydrogenases, 50) but the position of the amino acid sequences for the hemebinding domain and superbarrel domain found in this family are the reverse of those of the other members. This is considered significant in terms of the ability of PVA-DH to react with a macromolecule such as PVA.…”

Section: Microbial Degradation Of Synthetic Water-soluble Polymersmentioning

confidence: 98%

The Biochemistry and Molecular Biology of Xenobiotic Polymer Degradation by Microorganisms

Kawai

2010

Bioscience, Biotechnology, and Biochemistry

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Research on microbial degradation of xenobiotic polymers has been underway for more than 40 years. It has exploited a new field not only in applied microbiology but also in environmental microbiology, and has greatly contributed to polymer science by initiating the design of biodegradable polymers. Owing to the development of analytical tools and technology, molecular biological and biochemical advances have made it possible to prospect for degrading microorganisms in the environment and to determine the mechanisms involved in biodegradation when xenobiotic polymers are introduced into the environment and are exposed to microbial attack. In this review, the molecular biological and biochemical aspects of the microbial degradation of xenobiotic polymers are summarized, and possible applications of potent microorganisms, enzymes, and genes in environmental biotechnology are suggested.

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Section: Microbial Degradation Of Synthetic Water-soluble Polymersmentioning

confidence: 98%

The Biochemistry and Molecular Biology of Xenobiotic Polymer Degradation by Microorganisms

Kawai

2010

Bioscience, Biotechnology, and Biochemistry

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“…The PVADH is also designated with the systematic name polyvinyl alcohol: ferricytochrome-c oxidoreductase, which reflects the observation that the enzymatic oxidation of PVA is coupled to the respiratory chain of the microbe via the heme-containing redoxprotein cytochrome c. Such a coupling could not be observed with PVA oxidases. Although PVADH could be successfully cloned and heterologously expressed [75], a non-quino-hemoprotein PVA oxidase has not yet been cloned [76].…”

Section: Enzymesmentioning

confidence: 98%

Biodegradability of Poly(vinyl acetate) and Related Polymers

Amann

Minge

2011

Synthetic Biodegradable Polymers

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This chapter deals with the biodegradability of vinyl ester-based polymers with a special emphasis on poly(vinyl acetate) and poly(vinyl alcohol). A proper discussion of the importance of the biodegradability of a certain polymer class cannot be made without understanding the impact that polymer class has on the environment. Therefore, apart from discussing the actual biodegradation mechanisms, other issues will be addressed. These include, but are not limited to, how long the class of vinyl ester-based polymers has been known and produced on an industrial scale, what quantities are produced and released into the environment each year, and what applications are addressed with this polymer class. We will also look at the general physical and chemical properties of this polymer class and how these properties can influence biodegradability. After a discussion of what "biodegradability" actually means -and what not -the mechanisms for the biodegradation of poly(vinyl ester)s will be discussed in more detail and a summary given of the biological systems able to process poly(vinyl ester)s.

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“…VM15C [22,23] and Sphingopyxis sp. 113P3 [7,12,13] have been registered in GenBank. PVA-DH and OPH from these two strains have similar enzymatic properties and their amino acid sequences show high similarities, 54 and 63%, respectively.…”

Section: Introductionmentioning

confidence: 99%

Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli

Yang

Zhang

Liu

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Oxidized polyvinyl alcohol (PVA) hydrolase (OPH) is a key enzyme in the degradation of PVA, suggesting that OPH has a great potential for application in textile desizing processes. In this study, the OPH gene from Sphingopyxis sp. 113P3 was modified, by artificial synthesis, for overexpression in Escherichia coli. The OPH gene, lacking the sequence encoding the original signal peptide, was inserted into pET-20b (+) expression vector, which was then used to transform E. coli BL21 (DE3). OPH expression was detected in culture medium in which the transformed E. coli BL21 (DE3) was grown. Nutritional and environmental conditions were investigated for improved production of OPH protein by the recombinant strain. The highest OPH activity measured was 47.54 U/mL and was reached after 84 h under optimal fermentation conditions; this level is 2.64-fold higher that obtained under sub-optimal conditions. The productivity of recombinant OPH reached 565.95 U/L/h. The effect of glycine on the secretion of recombinant OPH was examined by adding glycine to the culture medium to a final concentration of 200 mM. This concentration of glycine reduced the fermentation time by 24 h and increased the productivity of recombinant OPH to 733.17 U/L/h. Our results suggest that the recombinant strain reported here has great potential for use in industrial applications.

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Cloning and expression of the gene for periplasmic poly(vinyl alcohol) dehydrogenase from Sphingomonas sp. strain 113P3, a novel-type quinohaemoprotein alcohol dehydrogenase

Cited by 33 publications

References 21 publications

The Biochemistry and Molecular Biology of Xenobiotic Polymer Degradation by Microorganisms

The Biochemistry and Molecular Biology of Xenobiotic Polymer Degradation by Microorganisms

Biodegradability of Poly(vinyl acetate) and Related Polymers

Expression and fermentation optimization of oxidized polyvinyl alcohol hydrolase in E. coli

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