Cloning and expression of the Campylobacter jejuni glyA gene in Escherichia coli

1995

“…The plasmids used in this study are listed in Table 1. The C. jejuni pBR322 genomic library was prepared from strain TGH9011 (ATCC 43431) as described previously (8).…”

Section: Methodsmentioning

confidence: 99%

Expression and characterization of Campylobacter jejuni benzoylglycine amidohydrolase (Hippuricase) gene in Escherichia coli

Hani

1995

“…The genomes of two different strains of C. jejuni were recently analyzed by field inversion gel electrophoresis or pulsed-field gel electrophoresis and were shown to be about 1,900 kb (18) and 1,700 kb (5,26). To date, only a few C. jejuni chromosomal genes have been cloned (4,17,20,25), and even fewer have been sequenced (3,27).…”

mentioning

confidence: 99%

Lysyl-tRNA synthetase gene of Campylobacter jejuni

Bingham

1992

We report the cloning and complete nucleotide sequence of the Campylobacter jejuni lysyl-tRNA synthetase gene (lysS). The C. jejuni lysS gene sequence shows high homology to the two Escherichia coli lysyl-tRNA synthetase genes, lysS and lysU. The Campylobacter lysyl-tRNA synthetase protein (LysRS) shows 47.9 and 46.6% sequence identity to the E. coli enzymes encoded by the lysS and lysU genes, respectively. The LysRS encoded by the C. jejuni gene is a polypeptide of 501 amino acids with a deduced molecular weight of 57,867. The enzyme is active in E. coli. The gene is expressed from its own promoter, and the transcription start site has been mapped. The carboxyl-terminal codon of the C. jejuni lysS gene overlaps by 1 bp with the Met initiation codon of the glyA gene, which has been shown to have a promoter which is functional in E. coli (V.L. Chan and H.L. Bingham, Gene 101:51-58, 1991). C. jejuni, unlike E. coli, has only one lysyl-tRNA synthetase gene.

show abstract

“…A 0.544-kb HindIll fragment containing a partial argH sequence as identified by a nucleotide data bank search (2) was isolated incidentally from a phage library and nick translated for use as a probe to screen a genomic pBR322 C. jejuni library to obtain the complete argH gene. The library was constructed as described previously (7). The argH gene was also retrieved from the C. jejuni library by complementation of an E. coli argH auxotrophic mutant (15 Complementation of an E. coli argH mutant.…”

Section: Resultsmentioning

confidence: 99%

“…The C. jejuni genomic library was constructed from strain TGH9011 (ATCC 43431) as described previously (7). The bacterial strains and plasmids used in this study are listed in Table 1.…”

Section: Materuils and Methodsmentioning

confidence: 99%

Cloning, characterization, and nucleotide sequence analysis of the argH gene from Campylobacter jejuni TGH9011 encoding argininosuccinate lyase

Hani

1994