Lysyl-tRNA synthetase gene of Campylobacter jejuni

Abstract

We report the cloning and complete nucleotide sequence of the Campylobacter jejuni lysyl-tRNA synthetase gene (lysS). The C. jejuni lysS gene sequence shows high homology to the two Escherichia coli lysyl-tRNA synthetase genes, lysS and lysU. The Campylobacter lysyl-tRNA synthetase protein (LysRS) shows 47.9 and 46.6% sequence identity to the E. coli enzymes encoded by the lysS and lysU genes, respectively. The LysRS encoded by the C. jejuni gene is a polypeptide of 501 amino acids with a deduced molecular weight of 57,867. The enzyme is active in E. coli. The gene is expressed from its own promoter, and the transcription start site has been mapped. The carboxyl-terminal codon of the C. jejuni lysS gene overlaps by 1 bp with the Met initiation codon of the glyA gene, which has been shown to have a promoter which is functional in E. coli (V.L. Chan and H.L. Bingham, Gene 101:51-58, 1991). C. jejuni, unlike E. coli, has only one lysyl-tRNA synthetase gene.

Expression and characterization of Campylobacter jejuni benzoylglycine amidohydrolase (Hippuricase) gene in Escherichia coli

“…Two of the theoretical open reading frames overlap by 16 nucleotides (ORFU2 and ORFL1) on complementary strands. Overlapping genes have already been described for C. jejuni (7,10). The intergenic regions between the coding sequences are small, which reflects the size of the genome of C. jejuni (17).…”

A Root‐Specific Iron‐Regulated Gene of Tomato Encodes a Lysyl‐tRNA‐Synthetase‐Like Protein

“…Another connection between the expression of lysyl-tRNA synthetase and iron metabolism has been found recently. The analysis of the gene coding for lysyl-tRNA synthetase in Cumpylobucter jejuni revealed a tight linkage between this gene and thefur gene (Chan and Bingham, 1992;Chan et al, 1995). The latter gene encodes a protein (FUR) that controls ferricuptake regulation in bacteria.…”

“…l), these three motives can be detected in the LELysRS gene product between amino acid positions 268-285, 327-351 and 551-583. In addition to these three motifs, the alignment identifies two highly conserved regions found in all class-I1 aminoacyl-tRNA synthetases (Chan and Bingham, 1992). One of these sequences is located upstream of motif I (amino acid positions 236-249) and is labelled A (Fig.…”

Iron-responsive genetic regulation in Campylobacter jejuni: cloning and characterization of a fur homolog

“…3) is probably the product of this gene. When comparing the nucleotide sequence of the C. jejuni fur gene with sequences deposited in the EMBU GenBank databases, we noticed that the sequence encoding the C-terminal 57 amino acids had been reported previously as the region upstream of the lysyl-tRNA synthetase gene (14). The coding sequences of the two genes are in frame and separated by a single stop codon.…”