Cloning and Functional Analysis of Geraniol 10-Hydroxylase, a Cytochrome P450 fromSwertia mussotiiFranch

Wang, Junfeng; Liu, Yanling; Cai, Yunfei; Zhang, Fangfang; Xia, Guangmin; Xiang, Fengning

doi:10.1271/bbb.100175

Cited by 44 publications

(40 citation statements)

References 21 publications

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“…Foliar application of methyl jasmonate (MeJ) has been shown to induce several different secondary metabolite biosynthetic pathways in both angiosperms and gymnosperms, with the terpenoid pathways often being highly induced (Ament et al 2004;Miller et al 2005;Rodriguez-Saona et al 2001;Wang et al 2010;Zulak et al 2009). In the present work, initial investigation into the foliar application of MeJ was carried out on ninemonth-old sandalwood saplings in the glasshouse ( Figure 1A).…”

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Foliar application of methyl jasmonate does not increase terpenoid accumulation, but weakly elicits terpenoid pathway genes in sandalwood (Santalum album L.) seedlings

Külheim

Jones

Ghisalberti

et al. 2014

Plant Biotechnology

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The sesquiterpenoid rich essential oils of sandalwood (Santalum album L.) stems and roots are a highly sought commodity in the fragrance industry. Plantations of sandalwood are being established in northern Australia, however the valuable heartwood essential oils do not accumulate in substantial amounts before 10 years, while commercially viable harvests do not normally take place for at least 15 years. Inducing essential oil accumulation at an earlier stage, or increasing oil yield in mature trees, may have the potential to enhance the oil productivity of plantations. In this study, we investigated the effects of foliar application of methyl jasmonate on less than one-year-old sandalwood seedlings. Essential oil accumulation was unaffected in both stems and roots. However, at the gene transcript level, several key genes early in the biosynthesis of sandalwood oil components were induced in both leaves and stems. These results suggest that terpenoid biosynthesis in S. album does indeed respond to foliar application of methyl jasmonate, however the effects are small and the full biosynthesis of santalols is likely to be developmentally regulated.

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confidence: 99%

Foliar application of methyl jasmonate does not increase terpenoid accumulation, but weakly elicits terpenoid pathway genes in sandalwood (Santalum album L.) seedlings

Külheim

Jones

Ghisalberti

et al. 2014

Plant Biotechnology

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“…These include CYP76B6 isolated from C. roseus (Collu et al, 2001;Ginis et al, 2012;Meijer et al, 1993), CYP76B10 from Swertia mussotii (Wang et al, 2010), CYP76A4 from Petunia x hybrida (Tamaki et al, 2005) and CYP76C1 (Mizutani and Ohta, 1998) and CYP76C4 (Hofer et al, 2013) from Arabidopsis thaliana. However, although it has been suggested that GOH hydroxylation could be a common characteristic of CYP76s http://dx.doi.org/10.1016/j.phytochem.2015.08.005 0031-9422/Ó 2015 Elsevier Ltd. All rights reserved.…”

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confidence: 99%

Functional expression of a putative geraniol 8-hydroxylase by reconstitution of bacterially expressed plant CYP76F45 and NADPH-cytochrome P450 reductase CPR I from Croton stellatopilosus Ohba

et al. 2015

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“…MeJA (100 lM) and YE (2-16 g L -1 ) can significantly enhance xanthone production in C. erythraea cell suspension cultures (Beerhues and Berger 1995). MeJA elicitation of secoiridoid production has not been reported in C. erythraea; however, elicitation of Swertia mussotii seedlings with 50 lM MeJA doubled the amount of Swam (Wang et al 2010). In conclusion, the use of LC-ESI-TOF MS for comparison of five major secondary metabolites in three different C. erythraea in vitro culture types with wild plants provided novel data on advantages of different culture types for production of secoiridoids and xanthones.…”

Section: Resultsmentioning

confidence: 97%

“…Elicitation is successfully applied for enhanced production of secondary metabolites in many species (Murthy et al 2014). Elicitation with methyljasmonate (MeJA) in related Gentianaceae species Swertia mussotii resulted in overexpression of geraniol 10-hydroxylase gene and concomitant accumulation of swartiamarin (Wang et al 2010), while chitosan elicited xanthone accumulation in Hypericum perforatum root cultures (Valletta et al 2016). The objective of this study was to use LC-ESI-TOF MS (liquid chromatography electrospray ionization time of flight mass spectrometry) to compare amount of secoiridoid and xanthone metabolites, total phenolic content (TPC) and antiradical activity (ARA) in ethanol-aqueous extracts from three types of C. erythraea in vitro cultures and wild plants.…”

Section: Introductionmentioning

confidence: 99%

In vitro culture type and elicitation affects secoiridoid and xanthone LC–ESI–TOF MS profile and production in Centaurium erythraea

Boroduske

Nakurte

Tomsone

et al. 2016

Plant Cell Tiss Organ Cult

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Centaurium erythraea is medicinal plant with high content of secoiridoid glycosides and xanthones with gastroprotective, hepatoprotective and antioxidative properties. In vitro culture based C. erythraea production would offer sustainable and economically feasible alternative to wild plant harvest provided that comparable productivity could be achieved in artificially propagated plants. To identify most productive C. erythraea system the secoiridoid glycoside and xanthone production and profiles using liquid chromatography-mass spectrometry were assessed in in vitro shoot, callus and cell suspension culture-derived material in comparison with wild C. erythraea plants.In vitro shoot culture was the most productive system for C. erythraea secoiridoid production (total yield 86 mg g -1 dw) and cell culture was the most productive system for phenolic compound production (19 mg GAE g -1 dw). The effect of elicitors yeast extract, methyljasmonate (MeJA) and chitosan on secondary metabolite yield indicated that elicitation with MeJA and chitosan significantly decreased yield of sweroside and eustomin, respectively. Significant quantitative differences of major secoiridoids and xanthones indicated that further methodological improvements for increasing yield of major secondary metabolites of in vitro origin need to be explored for in vitro cultivation to become a feasible alternative to C. erythraea wild plant harvest.

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Cloning and Functional Analysis of Geraniol 10-Hydroxylase, a Cytochrome P450 fromSwertia mussotiiFranch

Cited by 44 publications

References 21 publications

Foliar application of methyl jasmonate does not increase terpenoid accumulation, but weakly elicits terpenoid pathway genes in sandalwood (<i>Santalum album</i> L.) seedlings

Foliar application of methyl jasmonate does not increase terpenoid accumulation, but weakly elicits terpenoid pathway genes in sandalwood (<i>Santalum album</i> L.) seedlings

Functional expression of a putative geraniol 8-hydroxylase by reconstitution of bacterially expressed plant CYP76F45 and NADPH-cytochrome P450 reductase CPR I from Croton stellatopilosus Ohba

In vitro culture type and elicitation affects secoiridoid and xanthone LC–ESI–TOF MS profile and production in Centaurium erythraea

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