In vitro culture type and elicitation affects secoiridoid and xanthone LC–ESI–TOF MS profile and production in Centaurium erythraea

Abstract

Centaurium erythraea is medicinal plant with high content of secoiridoid glycosides and xanthones with gastroprotective, hepatoprotective and antioxidative properties. In vitro culture based C. erythraea production would offer sustainable and economically feasible alternative to wild plant harvest provided that comparable productivity could be achieved in artificially propagated plants. To identify most productive C. erythraea system the secoiridoid glycoside and xanthone production and profiles using liquid chromatography-mass spectrometry were assessed in in vitro shoot, callus and cell suspension culture-derived material in comparison with wild C. erythraea plants.In vitro shoot culture was the most productive system for C. erythraea secoiridoid production (total yield 86 mg g -1 dw) and cell culture was the most productive system for phenolic compound production (19 mg GAE g -1 dw). The effect of elicitors yeast extract, methyljasmonate (MeJA) and chitosan on secondary metabolite yield indicated that elicitation with MeJA and chitosan significantly decreased yield of sweroside and eustomin, respectively. Significant quantitative differences of major secoiridoids and xanthones indicated that further methodological improvements for increasing yield of major secondary metabolites of in vitro origin need to be explored for in vitro cultivation to become a feasible alternative to C. erythraea wild plant harvest.

Optimization of salicylic acid and chitosan treatment for bitter secoiridoid and xanthone glycosides production in shoot cultures of Swertia paniculata using response surface methodology and artificial neural network

“…Likewise, in other genera of Gentianaceae family, Krstic et al [35] described the higher production of xanthones and secoiridoids in organ cultures. Boroduske et al [36] advocated shoot culture more suitable for secoiridoid production.…”

“…Transcriptome studies revealed the elevated gene expressions involved in the metabolic pathways of secoiridoid and xanthone compounds. Research indicates that chitosan shows considerable increase of swertiamarin compound in Centaurium erythraea [36], amarogentin compound in Swertia chirata [12] and mangiferin compound in Hypericum perforatum cultured plants [42]. Chitosan is well known to release various defense genes and also to induce production of secondary metabolites in order to inhibit the growth of microbes [21,43].…”

A novel comparative chromatographic research of secoiridoid glycosides in two species of centaury herb

“…Its gastroprotective [5], hepatoprotective, diuretic effects and hypoglycemic activity [6] and other medical activities have been described before [7]. The main representatives secoiridoid glycosides in Centaury herb are swertiamarin, gentiopicroside andsweroside [8,9] (Fig. 1).…”

Suppression of Phyllospheric Microbiota Alters Content of Pharmacologically Relevant Compounds of <i>S. nigra</i> Flowers

“…Analysis was performed essentially as described in Ref. [12]. LC/MS data preprocessing was performed with MZmine 2.27 [13].…”