Cloning and functional characterization of HKT1 and AKT1 genes of Fragaria spp.—Relationship to plant response to salt stress

Garriga, Miguel; Raddatz, Natalia; Véry, Anne‐Aliénor; Sentenac, Hervé; Rubio-Meléndez, María E; González, Wendy; Drèyer, Ingo

doi:10.1016/j.jplph.2016.12.007

Cited by 43 publications

(24 citation statements)

References 106 publications

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“…Among them, HKT1 is a high-affinity K + transporter encoded in roots and leaves mainly by controlling Na + input in roots [49]. Garriga et al [50] conducted a functional study of AtHKT1 in the model plant Arabidopsis thaliana L. Heynh and found that AtHKT1, which mediates Na + transport, increases plant tolerance to salt. The Na + efflux was dependent on Na + /H + exchanger/antiporter localized to the plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

Salicylic Acid Alleviated Salt Damage of Populus euphratica: A Physiological and Transcriptomic Analysis

Rao

et al. 2019

Forests

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Populus euphratica Oliv. is a model tree for studying abiotic stress, especially salt stress response. Salt stress is one of the most extensive abiotic stresses, which has an adverse effect on plant growth and development. Salicylic acid (SA) is an important signaling molecule that plays an important role in modulating the plant responses to abiotic stresses. To answer whether the endogenous SA can be induced by salt stress, and whether SA effectively alleviates the negative effects of salt on poplar growth is the main purpose of the study. To elucidate the effects of SA and salt stress on the growth of P. euphratica, we examined the morphological and physiological changes of P. euphratica under 300 mM NaCl after treatment with different concentrations of SA. A pretreatment of P. euphratica with 0.4 mM SA for 3 days effectively improved the growth status of plants under subsequent salt stress. These results indicate that appropriate concentrations of exogenous SA can effectively counteract the negative effect of salt stress on growth and development. Subsequently, transcripts involved in salt stress response via SA signaling were captured by RNA sequencing. The results indicated that numerous specific genes encoding mitogen-activated protein kinase, calcium-dependent protein kinase, and antioxidant enzymes were upregulated. Potassium transporters and Na+/H+ antiporters, which maintain K+/Na+ balance, were also upregulated after SA pretreatment. The transcriptome changes show that the ion transport and antioxidant enzymes were the early enhanced systems in response of P. euphratica to salt via SA, expanding our knowledge about SA function in salt stress defense in P. euphratica. This provides a solid foundation for future study of functional genes controlling effective components in metabolic pathways of trees.

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Section: Discussionmentioning

confidence: 99%

Salicylic Acid Alleviated Salt Damage of Populus euphratica: A Physiological and Transcriptomic Analysis

Rao

et al. 2019

Forests

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“…Solanum lycopersicum plants become hypersensitivity to salinity with ScHKT1;2 and SlHKT1;2 genes silencing 36 . In strawberry ( Fragaria ananassa ), an increase of HKT1 expression in roots leads to high tolerance to salinity 37 . Most of them focused on the HKT1 function on Na + accumulation under salt stress.…”

Section: Discussionmentioning

confidence: 99%

AtHKT1 gene regulating K+ state in whole plant improves salt tolerance in transgenic tobacco plants

Wang

Liu

Feng

et al. 2018

Sci Rep

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The status of K+ is important for plant health. However, little is known about if high-affinity potassium transporter HKTs may help K+ retention under salt stress. Here, we determined the effect of Arabidopsis thaliana transporter gene (AtHKT1) on the K+ status, Na+-induced toxicity, and salt tolerance in tobacco (Nicotiana tabacum L.). Six AtHKT1 transformed tobacco lines (T1, T2, … T6) were contrasted with a non-transgenic plantlet at the whole-plant and molecule levels. AtHKT1 gene was expressed in the xylems of stem, root and leaf vein in the transgenic tobacco, with the line T3 having highest expression. At Day 15, in the 200 mmol L−1 NaCl stress treatment, the transgenic plants remained a healthy K+ status, while the control plants decreased K+ content by 70% and Na+ contents in leaves and stems were 1.7 times that in the transgenic line. The AtHKT1 expression enhanced the activities of SOD, CAT and POD, raised chlorophyll and soluble sugar contents and root activity, and decreased MDA and proline contents and electrolyte leakage destruction. The constitutive over-expression of AtHKT1 that helps maintain a healthy K+ status while reducing Na+ toxicity may serve as a possible mechanism in maximizing productivity of tobacco under salt stress.

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“…Sodium (Na + )-stress tolerance in plants includes at least three processes: compartmentalization (at the cellular and/or tissue levels), exclusion (from shoots into roots or from roots into the rhizosphere), and long-distant transport (Munns and Tester, 2008 ; Plett and Møller, 2010 ; Suzuki et al, 2016a , b ; Negrão et al, 2017 ). Several molecular mechanisms underlying salinity stress tolerance have been found in glycophytic plants, such as, rice, wheat, and Arabidopsis (Apse et al, 1999 ; Halfter et al, 2000 ; Shi et al, 2002 ; Sunarpi et al, 2005 ; James et al, 2006 ; Sahi et al, 2006 ; Brini et al, 2007 ; Horie et al, 2007 ; Garriga et al, 2016 ; Suzuki et al, 2016a , b ; Zhang et al, 2016 ; Negrão et al, 2017 ). For example, NHX genes are responsible for Na + compartmentalization in vacuoles; both the rice HKT1;5 gene and wheat HKT1;5 gene function in Na + exclusion from shoots into roots; the Arabidopsis SOS system mediates Na + exclusion into the rhizosphere (Munns and Tester, 2008 ; Plett and Møller, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Profiling and Physiological Responses of Halophyte Kochia sieversiana Provide Insights into Salt Tolerance

et al. 2017

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Halophytes are remarkable plants that can tolerate extremely high-salinity conditions, and have different salinity tolerance mechanisms from those of glycophytic plants. In this work, we investigated the mechanisms of salinity tolerance of an extreme halophyte, Kochia sieversiana (Pall.) C. A. M, using RNA sequencing and physiological tests. The results showed that moderate salinity stimulated the growth and water uptake of K. sieversiana and, even under 480-mM salinity condition, K. sieversiana maintained an extremely high water content. This high water content may be a specific adaptive strategy of K. sieversiana to high salinity. The physiological analysis indicated that increasing succulence and great accumulations of sodium, alanine, sucrose, and maltose may be favorable to the water uptake and osmotic regulation of K. sieversiana under high-salinity stress. Transcriptome data indicated that some aquaporin genes and potassium (K+) transporter genes may be important for water uptake and ion balance, respectively, while different members of those gene families were employed under low- and high-salinity stresses. In addition, several aquaporin genes were up-regulated in low- but not high-salinity stressed roots. The highly expressed aquaporin genes may allow low-salinity stressed K. sieversiana plants to uptake more water than control plants. The leaf K+/root K+ ratio was enhanced under low- but not high-salinity stress, which suggested that low salinity might promote K+ transport from the roots to the shoots. Hence, we speculated that low salinity might allow K. sieversiana to uptake more water and transport more K+ from roots to shoots, increasing the growth rate of K. sieversiana.

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Cloning and functional characterization of HKT1 and AKT1 genes of Fragaria spp.—Relationship to plant response to salt stress

Cited by 43 publications

References 106 publications

Salicylic Acid Alleviated Salt Damage of Populus euphratica: A Physiological and Transcriptomic Analysis

Salicylic Acid Alleviated Salt Damage of Populus euphratica: A Physiological and Transcriptomic Analysis

AtHKT1 gene regulating K+ state in whole plant improves salt tolerance in transgenic tobacco plants

Transcriptomic Profiling and Physiological Responses of Halophyte Kochia sieversiana Provide Insights into Salt Tolerance

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