Cloning and genetic organization of the gene cluster encoding F7 fimbriae of a uropathogenic and comparison with the F7 gene cluster

Molecular cloning of the F8 fimbrial antigen from Escherichia coli

“…5.0 and 8.5 kb [8][9][10][11][12][13][14]23]. Therefore, it is suggested that the region coding for F8 fimbriae is smaller than the 12-kb segment inserted into pBR322.…”

“…On the basis of the restriction sites, no similarities were revealed between the cloned Sfimbrial antigen ( sfa) determinant and the F8 genes [23). Some restriction sites, however, such as a C/ai site and a Kpni site, seem tobe conserved in different cloned P-fimbrial determinants coding for F8, F9, F11, F7 1 , F13 or F 'Clegg' [8][9][10][11][12][13][14]. It therefore appears that a 6.0-kb DNA fragment which resulted from a Kpni-Hindiii double cleavage of pANN921 carried most of the F8 coding region.…”

“…As a probe, a 6.0-kb Kpn 1-Hindlll fragment was chosen. As judged from the restriction maps of other cloned F determinants, which share sequence homology [8][9][10][11][12][13][14], this fragment should carry most of the intemal F8 coding region.· As shown in Fig. 3, identical Pst I fragments were obtained from the chromosomal and the plasmid DNA.…”

“…p fimbriae can be divided into serologically distinct groups, termed F7-F13, but all recognize the a-~r.Gal (1-4)-ß-n-Gal region of globotetraosylceramide and trihexosylceramide, which are an tigens of the human blood group P system [7]. Some of these specific F-antigens have been cloned very receritly [8][9][10][11][12][13] and one of them, the Fl3 fimbria (also called pap ), has been studied in detail (14]. Here we describe the molecular cloning and charac~eri zation of the F8 fimbrial antigen from an 018: K5: H5: F1C, F8 wild-type isolate 2980.…”

A novel lectin‐independent interaction of P fimbriae of Escherichia coli with immobilized fibronectin

“…coli HBI01 and the recombinant strains carrying cloned genes for the wild-type P (F71) fimbriae [HBI01(pP1L110-75)] or for the lectin-deficient P fimbriae [HB101(pPILI10-7501)] have been described before [20,21]. The strain IHl1128, possessing the O75X adhesin, has previously been characterized for its adhesins and binding properties [22,23].…”