Cloning and Heterologous Expression of Hematin-Dependent Catalase Produced by
            <i>Lactobacillus plantarum</i>
            CNRZ 1228

Abriouel, Hikmate; Herrmann, Anette; Stärke, Joachim; Yousif, N.M.K.; Wijaya, Agus; Tauscher, B.; Holzapfel, Wilhelm H.; Franz, Charles M. A. P.

doi:10.1128/aem.70.1.603-606.2004

Cited by 45 publications

(35 citation statements)

References 20 publications

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“…Since the majority of LAB are not equipped with enzymes to detoxify oxygen-derived compounds, the insertion of genes coding for antioxidant enzymes (such as catalases or SOD) in probiotic bacteria could improve their anti-inflammatory properties beyond the modulation of the local immunedependant inflammation response. Catalases of three lactobacilli have been successfully cloned and expressed in heterologous bacteria lacking catalase activity [62][63][64][65].…”

Section: Genetically Engineered Lab-antioxidant Enzyme and Il-10 Prodmentioning

confidence: 99%

Mechanisms Involved in the Anti-Inflammatory Properties of Native and Genetically Engineered Lactic Acid Bacteria

LeBlanc¹,

Carmen²,

Zurita-Turk³

et al. 2012

AIA

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Lactic acid bacteria (LAB) represent a heterogeneous group of microorganisms that have been shown to possess therapeutic properties since they are able to prevent the development of some diseases, as shown mostly on animal models for cancer, infections and gastrointestinal disorders such as intestinal inflammation. LAB have been shown to regulate mucosal immune responses by modulating the production and liberation of regulatory agents such as cytokines by the host. Some of these cytokines, such as the anti-inflammatory interleukin-10 (IL-10), modulate the inflammatory immune response, thus immunomodulation is a mechanism by which LAB can prevent certain inflammatory bowel diseases (IBD). Since oxidative stress participates in the inflammatory processes and in the appearance of damages in pathologies of the gastrointestinal tract of humans such as IBD, LAB could also prevent inflammation by eliminating reactive oxygen species (ROS) through the activity of antioxidant enzymes. In order to obtain novel strains or enhance beneficial effects of LAB, genetic engineering has been used to produce either antioxidant enzymes (such as catalases and superoxide dismutases) or anti-inflammatory cytokines (such as IL-10) producing LAB. These novel strains have successfully been used to prevent inflammatory bowel diseases in animal models and could be evaluated in human clinical trials. Here, we present an overview of the current knowledge of the mechanisms by which LAB can be used to prevent undesired intestinal inflammatory responses and could be used as a therapeutic tool for IBD.

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Section: Genetically Engineered Lab-antioxidant Enzyme and Il-10 Prodmentioning

confidence: 99%

Mechanisms Involved in the Anti-Inflammatory Properties of Native and Genetically Engineered Lactic Acid Bacteria

LeBlanc¹,

Carmen²,

Zurita-Turk³

et al. 2012

AIA

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“…H 2 O 2 can subsequently be decomposed by the action of catalases. Although lactic acid bacteria are generally considered to be catalase-negative, catalase activity has been reported for several species, including members of the genera Lactobacillus, Pediococcus and Leuconostoc (Delwiche, 1961;Knauf et al, 1992;Abriouel et al, 2004). There are two major classes of catalases: true or haemdependent catalase, and manganese-containing catalase or pseudocatalase (Kono & Fridovich, 1983a, b).…”

Section: Introductionmentioning

confidence: 99%

Effect of respiration and manganese on oxidative stress resistance of Lactobacillus plantarum WCFS1

et al. 2012

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Lactobacillus plantarum is a facultatively anaerobic bacterium that can perform respiration under aerobic conditions in the presence of haem, with vitamin K 2 acting as a source of menaquinone. We investigated growth performance and oxidative stress resistance of Lb. plantarum WCFS1 cultures grown in de Man, Rogosa and Sharpe (MRS) medium without and with added manganese under fermentative, aerobic, aerobic with haem, and respiratory conditions. Previous studies showed that Lb. plantarum WCFS1 lacks a superoxide dismutase and requires high levels of manganese for optimum fermentative and aerobic growth. In this study, respiratory growth with added manganese resulted in significantly higher cell densities compared to the other growth conditions, while without manganese added, similar but lower cell densities were reached. Notably, cells derived from the respiratory cultures showed the highest hydrogen peroxide resistance in all conditions tested, although similar activity levels of haem-dependent catalase were detected in cells grown under aerobic conditions with haem. These results indicate that oxidative stress resistance of Lb. plantarum is affected by respiratory growth, growth phase, haem and manganese. As levels of haem and manganese can differ considerably in the raw materials used in fermentation processes, including those of milk, meat and vegetables, the insight gained here may provide tools to increase the performance and robustness of starter bacteria.

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“…Although heterologous catalases have been expressed in sev- (1,10,16,18), this work constitutes the first report of heterologous expression of a nonheme catalase in bacteria relevant to dairy industries, offering the advantage that no heme has to be added to the culture medium for enzyme activity. In addition, this is the first demonstration of the protection of a bacterial species from H 2 O 2 damage by the production of a heterologous catalase by its partner in mixed fermentations.…”

Section: Discussionmentioning

confidence: 99%

Production of a Heterologous Nonheme Catalase by Lactobacillus casei : an Efficient Tool for Removal of H ₂ O ₂ and Protection of Lactobacillus bulgaricus from Oxidative Stress in Milk

Rochat

Gratadoux

Gruss³

et al. 2006

Appl Environ Microbiol

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Cloning and Heterologous Expression of Hematin-Dependent Catalase Produced by Lactobacillus plantarum CNRZ 1228

Cited by 45 publications

References 20 publications

Mechanisms Involved in the Anti-Inflammatory Properties of Native and Genetically Engineered Lactic Acid Bacteria

Mechanisms Involved in the Anti-Inflammatory Properties of Native and Genetically Engineered Lactic Acid Bacteria

Effect of respiration and manganese on oxidative stress resistance of Lactobacillus plantarum WCFS1

Production of a Heterologous Nonheme Catalase by Lactobacillus casei : an Efficient Tool for Removal of H ₂ O ₂ and Protection of Lactobacillus bulgaricus from Oxidative Stress in Milk

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Cloning and Heterologous Expression of Hematin-Dependent Catalase Produced by Lactobacillus plantarum CNRZ 1228

Cited by 45 publications

References 20 publications

Mechanisms Involved in the Anti-Inflammatory Properties of Native and Genetically Engineered Lactic Acid Bacteria

Mechanisms Involved in the Anti-Inflammatory Properties of Native and Genetically Engineered Lactic Acid Bacteria

Effect of respiration and manganese on oxidative stress resistance of Lactobacillus plantarum WCFS1

Production of a Heterologous Nonheme Catalase by Lactobacillus casei : an Efficient Tool for Removal of H 2 O 2 and Protection of Lactobacillus bulgaricus from Oxidative Stress in Milk

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Production of a Heterologous Nonheme Catalase by Lactobacillus casei : an Efficient Tool for Removal of H ₂ O ₂ and Protection of Lactobacillus bulgaricus from Oxidative Stress in Milk