Cloning and primary immunological study of TGF-β1 and its receptors TβR I /TβR II in tilapia(Oreochromis niloticus)

Zhan, Xu-liang; Ma, Ting; Wu, Jinying; Yi, Liyuan; Wang, Jing‐Yuan; Gao, Xiao-ke; Li, Wensheng

doi:10.1016/j.dci.2015.03.008

Cited by 26 publications

(9 citation statements)

References 44 publications

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“…Despite the divergence in their sequence, the serine/threonine kinase domains, which play a crucial role in ligand recognition, are conserved in the ALK5 and BMPR2 amino acid sequences, similar to the results observed in buffalo [ 8 ] and O. mykiss [ 12 ]. All of the results reveal the relatively high homogeneity of the different kinase domains and sequences with those of other vertebrates, suggesting that ALK5 and BMPR2 play conserved roles in signal transduction [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…The expression of ALK5 and BMPR2 was detected in almost all tissues, particularly in gonads. Similarly, ALK5 is expressed in all tissues of O. mykiss [ 12 ] and is expressed at high levels in the buffalo ovary [ 8 ], the liver of O. niloticus [ 13 ] and C. gibelio follicle cells [ 14 ]. During oocytes development, the expression of ALK5 in ovaries at the early vitellogenic stage is higher than that at other stages [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…During the development of the corpus luteum (CL) from early to late CL, the expression of ALK5 continually increases [ 11 ]. In fish, ALK5 , which is abundant in gonads and muscles, is distributed in the tissues of Oncorhynchus mykiss [ 12 ] and mainly expressed in the liver of Oreochromis niloticus [ 13 ] and Carassius gibelio [ 14 ]. These results indicated that the expression of ALK5 varies in different species.…”

Section: Introductionmentioning

confidence: 99%

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Estradiol Upregulates the Expression of the TGF-β Receptors ALK5 and BMPR2 during the Gonadal Development of Schizothorax prenanti

Yan

Zhang

Cai

et al. 2021

Animals

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TGF-β receptors play important roles in mediating TGF-β signals during gonadal development. To identify the functions of TGF-β receptors, including the type I receptor (activin receptor-like kinase 5, ALK5) and type II receptor (bone morphogenetic protein receptor 2, BMPR2), during the gonadal development of S. prenanti, the full-length cDNA sequences of ALK5 and BMPR2 were isolated and characterized. Their expression patterns in developing gonads and in the gonads of exogenous estradiol (E2) -fed fish were analyzed. The cDNAs of ALK5 and BMPR2 were 1925 bp and 3704 bp in length and encoded 501 and 1070 amino acid residues, respectively. ALK5 and BMPR2 were mostly expressed in gonads, particularly in cortical alveoli stage ovaries and mid-spermatogenic stage testes; however, the overall level of BMPR2 mRNA was higher than that of ALK5 during gonadal development. Furthermore, immunohistochemical signals of ALK5 and BMPR2 were mostly detected at chromatin nucleolar oocytes and perinuclear oocytes in ovaries and at spermatocytes and spermatogonia in testes. Exogenous E2 induces the gonadal expression of ALK5 and BMPR2, and BMPR2 is more responsive to E2 than ALK5. These results suggest that ALK5 and BMPR2 might play a potentially vital role in both folliculogenesis and spermatogenesis in S. prenanti.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Estradiol Upregulates the Expression of the TGF-β Receptors ALK5 and BMPR2 during the Gonadal Development of Schizothorax prenanti

Yan

Zhang

Cai

et al. 2021

Animals

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“…Indeed, there is an increased level of (active) TGF-β1 protein and up-regulation of TGF-β signalling in the Antarctic notothenioid fish that lack hemoglobin/erythrocytes, compared to red-blooded notothenioids. TGF-β1 protein has also been detected in tilapia, where it was reported to increase from 12 h to three days in the HK, and from one to three days in spleen post-injection with Streptococcus agalactiae [ 148 ].…”

Section: Cysteine Knot Cytokinesmentioning

confidence: 99%

The Function of Fish Cytokines

Zou

Secombes

2016

Biology

448

269

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What is known about the biological activity of fish cytokines is reviewed. Most of the functional studies performed to date have been in teleost fish, and have focused on the induced effects of cytokine recombinant proteins, or have used loss- and gain-of-function experiments in zebrafish. Such studies begin to tell us about the role of these molecules in the regulation of fish immune responses and whether they are similar or divergent to the well-characterised functions of mammalian cytokines. This knowledge will aid our ability to determine and modulate the pathways leading to protective immunity, to improve fish health in aquaculture.

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“…Although effectual studies have been performed on the mode of action of RES, only a few relevant signal pathways have been identified, such as the oxygen (Wang L. et al, 2016), cytokine (Zheng et al, 2017b), apoptosis (Taylor et al, 2001), and DNA damage pathway (Wang et al, 2015). To select the target gene for verifying the effective pathway, researchers have performed gene cloning (Zhan et al, 2015), transcriptome analysis (Wang L. et al, 2016;Zhu et al, 2017), and miR-Seq (Qiang et al, 2017) in the corresponding organs, such as the liver (Zheng et al, 2017b), fin, kidney (Sutherland et al, 2014), spleen (Krasnov et al, 2013), and blood.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Juvenile Tilapia (Oreochromis niloticus) Blood, Fed With Different Concentrations of Resveratrol

Zheng

et al. 2020

Front. Physiol.

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Oreochromis niloticus (genetically improved farmed tilapia, GIFT) often bites the root of Polygonum cuspidatum when it is used as a floating bed, and resveratrol (RES) is mainly accumulated in the root of P. cuspidatum. Blood acts as a pipeline for the fish immune system. Generating blood transcriptomic resources is crucial for understanding molecular mechanisms underlying blood immune responses. In this study, we determined the effects of RES administration on blood transcriptomic response in GIFT. With increasing RES concentration, 133 (0.025 vs. 0.05 g/kg RES), 155 (0.025 vs. 0.1 g/kg RES), and 123 (0.05 vs. 0.1 g/kg RES) genes were detected as significant differentially expressed genes (DEGs). Three and ninety-five shared significant DEGs were found to be enriched among the three (except 0.1 g/kg RES) and four groups (0, 0.025, 0.05, and 0.1 g/kg RES), respectively. To determine the relationship between mitochondrial regulation and RES supplementation, the results of RNA-Seq were analyzed and nine mitochondria-related genes (ATP synthase or mitochondrial-function-related genes) were verified. The results revealed the same expression pattern: cytochrome c isoform X2 (cox2), katanin p60 ATPase-containing subunit A1 isoform X1 (katna1), plasma membrane calcium-transporting ATPase 1-like (atp2b1) and GTP-binding protein A-like (gtpbpal) showed the highest expression in the 0.1 g/kg RES group, while NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 mitochondrial (nad7), ATP synthase subunit beta, mitochondrial (atpb), ATP synthase subunit alpha, mitochondrial-like (atpal), ATP synthase subunit alpha, mitochondrial (atpa) and ATP-dependent Clp protease proteolytic subunit, mitochondrial (clpp) revealed a dose-dependent expression following RES supplementation. Blood Ca2+-ATPase activity, and malondialdehyde, glutathione, and ATP content were significantly increased in the 0.05 (except Ca2+-ATPase activity), 0.1 g/kg RES group when compared with the controls. Eighty-nine shared DGEs were mainly enriched in antigen processing and presentation, cell adhesion molecules and phagosome pathways, based on the comparison between previous reported hepatic and the present blood transcriptome. Our study demonstrated that RES supplementation might improve the resistance to metabolism dysfunction via mitochondrial energy synthesis and/or the respiratory chain (e.g., ATPase).

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Cloning and primary immunological study of TGF-β1 and its receptors TβR I /TβR II in tilapia(Oreochromis niloticus)

Cited by 26 publications

References 44 publications

Estradiol Upregulates the Expression of the TGF-β Receptors ALK5 and BMPR2 during the Gonadal Development of Schizothorax prenanti

Estradiol Upregulates the Expression of the TGF-β Receptors ALK5 and BMPR2 during the Gonadal Development of Schizothorax prenanti

The Function of Fish Cytokines

Transcriptome Analysis of Juvenile Tilapia (Oreochromis niloticus) Blood, Fed With Different Concentrations of Resveratrol

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