Cloning and recombinant expression of a barred sand bass (Paralabrax nebulifer) cDNA. The encoded protein displays structural homology and immunological crossreactivity to human complement/cofactor related plasma proteins

Zipfel, Peter F.; Kemper, Claudia; Dahmen, Antje; Gigli, Irma

doi:10.1016/s0145-305x(96)00025-0

Developmental & Comparative Immunology

1996

DOI: 10.1016/s0145-305x(96)00025-0

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Cloning and recombinant expression of a barred sand bass (Paralabrax nebulifer) cDNA. The encoded protein displays structural homology and immunological crossreactivity to human complement/cofactor related plasma proteins

Peter F. Zipfel

Claudia Kemper

Antje Dahmen

et al.

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Cited by 21 publications

(14 citation statements)

References 25 publications

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“…Loci of each group form a compact cluster, and, in the human genome, the group 1 cluster and the group 2 loci are also located in proximity, forming a RCA gene cluster (20)(21)(22). Genomic arrangement of the RCA genes and their clustering are diverse among species analyzed to date, such as mice (23,24), chicken (25,26), frog (27), and teleost, which is the most common group of the ray-finned bony fish (28)(29)(30)(31)(32)(33)(34).…”

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confidence: 99%

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A CD46-like Molecule Functional in Teleost Fish Represents an Ancestral Form of Membrane-Bound Regulators of Complement Activation

Tsujikura

Nagasawa

Ichiki

et al. 2015

The Journal of Immunology

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In the complement system, the regulators of complement activation (RCA) play crucial roles in controlling excessive complement activation and in protecting host cell from misdirected attack of complement. Several members of RCA family have been cloned from cyclostome and bony fish species and classified into soluble and membrane-bound type as in mammalian RCA factors. Complement-regulatory functions have been described only for soluble RCA of lamprey and barred sand bass; however, little is known on the biological function of the membrane-bound RCA proteins in the lower vertebrates. In this study, a membrane-bound RCA protein, designated teleost complement-regulatory membrane protein (Tecrem), was cloned and characterized for its complement-regulatory roles. Carp Tecrem, an ortholog of a zebrafish type 2 RCA, ZCR1, consists of four short consensus repeat modules, a serine/threonine/proline-rich domain, a transmembrane region, and a cytoplasmic domain, from the N terminus, as does mammalian CD46. Tecrem showed a ubiquitous mRNA expression in carp tissues, agreeing well with the putative regulatory role in complement activation. A recombinant Chinese hamster ovary cell line bearing carp Tecrem showed a significantly higher tolerance against lytic activity of carp complement and less deposition of C3-S, the major C3 isotypes acting on the target cell, than control Chinese hamster ovary (mock transfectant). Anti-Tecrem mAb enhanced the depositions of carp C3 and two C4 isotypes on autologous erythrocytes. Thus, the present findings provide the evidence of complement regulation by a membrane-bound group 2 RCA in bony fish, implying the host–cell protection is an evolutionarily conserved mechanism in regulation of the complement system.

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confidence: 99%

“…As for function of RCA in the lower vertebrates, regulatory roles in deposition of C3 and degradation of C3b/C4b have been described only for secreted RCAs in lamprey (35) and barred sand bass (28)(29)(30). No functional data, in contrast, have been reported for membrane-bound RCA protein in any lower vertebrate species.…”

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confidence: 99%

A CD46-like Molecule Functional in Teleost Fish Represents an Ancestral Form of Membrane-Bound Regulators of Complement Activation

Tsujikura

Nagasawa

Ichiki

et al. 2015

The Journal of Immunology

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“…Several factor-related molecules have been identified in sand bass plasma, and in a cDNA library prepared from the liver, one of these molecules was characterized in detail. The corresponding cDNA (termed c71) was cloned and sequenced [38]. It encodes an open reading frame of 204 amino acids and represents a secreted 24-kD protein composed of 3 SCR domains termed sand bass cofactor-related protein 1 (SBCFR-1).…”

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confidence: 99%

“…The functional characterization of the human FHR and SBCRP-1 proteins is under investigation. It has recently been shown that the FHR proteins have complement regulatory functions: both FHR-3 and FHR-4 bind C3b [38,44]. FHR-3 binds to heparin and the streptococcal M protein [25,39], and FHR-4 is associated in plasma with triglyceride-rich lipoproteins [32].…”

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confidence: 99%

Conservation of Plasma Regulatory Proteins of the Complement System in Evolution: Humans and Fish

Kemper

Gigli

Zipfel

2000

Exp Clin Immunogenet

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The complement system is an important defense system of innate immunity. The recent identification of structurally and functionally related complement regulatory proteins in the teleost, barred sand bass (Paralabrax nebulifer), and humans, two species which are separated in evolution by 100 million years, indicates a high level of conservation and the early presence of this defense system in evolution. The complement regulatory protein of barred sand bass, SBP1, is related to both the human alternative pathway regulator factor H, and to the classical pathway regulator C4bp, and displays regulatory activities in both human pathways. In addition, molecules with homology to the recently identified human factor-H-related proteins are also present in the sand bass genome. Here, we summarize the structural and functional aspects of these homologies and discuss the consequences for the evolution of the complement system.

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“…Dahmen et al (1994) and Zipfel et al (1996) cloned and analyzed two complement regulatory proteins from the barred sand bass Paralabrax neblifer, namely SBP1 and SBCFR-1. Recently, we isolated a complement regulatory protein-like cDNA from the Japanese floun der Paralichthys olivaceus, which we termed HCRF (Katagiri et al, 1998).…”

mentioning

confidence: 99%

Molecular Analysis of Complement Regulatory Protein-Like cDNA Composed of 12 Tandem SCRs from the Japanese Flounder.

Katagiri¹,

Hirono²,

Aoki³

1998

Fish Pathol.

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We cloned and analyzed a complement regulatory protein-like cDNA termed HCRF-2 from a liver cDNA library of the Japanese flounder Paralichthys olivaceus.The cDNA was 3101 bp in length and the predicted trans lation product of 737 amino acids contains a hydrophobic signal sequence followed by a stretch of 12 short consensus repeats (SCRs).These SCRs were closely related to those of the previously described SBP1 of complement regulatory plasma protein of barred sand bass. SCR domains Nos. 1 to 12 of HCRF-2 resembled domains Nos. 1 to 10 of SBP1. Recently, we reported a complement regulatory protein-like cDNA designated HCRF that contained 7 SCRs. SCR domains Nos. 2 to 7 of HCRF resembled domains Nos. 12 to 17 of SBP1, but SCR domains of HCRF did not resemble those of HCRF-2.In these comparisons, schematic models of HCRF, HCRF-2 and SBP1 clearly showed their close relationship, indicating that these cDNAs originated from the same ancestral gene.

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Cloning and recombinant expression of a barred sand bass (Paralabrax nebulifer) cDNA. The encoded protein displays structural homology and immunological crossreactivity to human complement/cofactor related plasma proteins

Cited by 21 publications

References 25 publications

A CD46-like Molecule Functional in Teleost Fish Represents an Ancestral Form of Membrane-Bound Regulators of Complement Activation

A CD46-like Molecule Functional in Teleost Fish Represents an Ancestral Form of Membrane-Bound Regulators of Complement Activation

Conservation of Plasma Regulatory Proteins of the Complement System in Evolution: Humans and Fish

Molecular Analysis of Complement Regulatory Protein-Like cDNA Composed of 12 Tandem SCRs from the Japanese Flounder.

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