Cloning and sequence of the cDNA encoding the rat oligodendrocyte integrin β1 subunit

Malek-Hedayat, Shahnaz; Rome, Leonard H.

doi:10.1016/0378-1119(94)00911-b

Cited by 15 publications

(9 citation statements)

References 8 publications

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“…The ␤ 1 -integrin mRNA was determined to be about 3 kb ( Fig. 2A) as expected from previous cloning data reporting the ␤ 1 -integrin coding region to be 2.4 kb in rat oligodendrocytes (23).…”

Section: Asupporting

confidence: 59%

Y Receptor-mediated Induction of CD63 Transcripts, a Tetraspanin Determined To Be Necessary for Differentiation of the Intestinal Epithelial Cell Line, hBRIE 380i Cells

Halldén¹,

Hadi²,

Hong³

et al. 1999

Journal of Biological Chemistry

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Peptide YY (PYY) and neuropeptide Y (NPY) are peptides that coordinate intestinal activities in response to luminal and neuronal signals. In this study, using the rat hybrid small intestinal epithelial cell line, hBRIE 380i cells, we demonstrated that PYY-and NPY-induced rearrangement of actin filaments may be in part through a Y1␣ and/or a nonneuronal Y2 receptor, which were cloned from both the intestinal mucosa and the hBRIE 380i cells. A number of PYY/NPY-responsive genes were also identified by subtractive hybridization of the hBRIE 380i cells in the presence or absence of a 6-h treatment with PYY. Several of these genes coded for proteins associated with the cell cytoskeleton or extracellular matrix. One of these proteins was the transmembrane-4 superfamily protein CD63, previously shown to associate with ␤ 1 -integrin and implicated in cell adhesion. CD63 immunoreactivity, using antibody to the extracellular domain, was highest in the differentiated cell clusters of the hBRIE 380i cells. The hBRIE 380i cells transfected with antisense CD63 cDNA lost these differentiated clusters. These studies suggest a new role for NPY and PYY in modulating differentiation through cytoskeletal associated proteins.Regulatory peptides common to the intestine and nerves (neuro-gut regulatory peptides) are unique chemical mediators released from receptosecretory and/or neurosecretory cells having multiple biological actions. These peptides can act as neurotransmitters, neurocrine, endocrine, or paracrine agents and share a common function in the coordination of intestinal activities in response to luminal signals or signals originating from peripheral tissues. Despite the range of diversity of their biological effects, there is one recurring biological observation that seems to link many of these peptides, their ability to act as factors affecting growth or differentiation of the intestinal mucosa. Differentiation and regulation of growth of complex tissues, such as the intestine, are not only determined by growth factors but also by intracellular signals generated by the interactions between cells and their extracellular matrix (ECM) 1 (1).In intestinal epithelial cells, part of the ECM occurs in the form of a basement membrane that provides these cells with positional information and signals that initiate the organization of intracellular structure and cellular behavior. A factor probably important for the positional cues for differentiation in the intestinal mucosa is the potential link between the ECM of the lamina propria and the process of cellular migration from crypt to villus. Although over the past years there have been studies on the growth effects for specific peptides such as cholecystokinin (2), bombesin (3), vasopressin, gastrin (4, 5), and peptide YY (PYY) (6), to name a few, the contribution of these peptides to intestinal differentiation remains relatively unexplored. Such investigations were limited in the past primarily due to the lack of an appropriate cell culture model that mimicked some of the critica...

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Section: Asupporting

confidence: 59%

Y Receptor-mediated Induction of CD63 Transcripts, a Tetraspanin Determined To Be Necessary for Differentiation of the Intestinal Epithelial Cell Line, hBRIE 380i Cells

Halldén¹,

Hadi²,

Hong³

et al. 1999

Journal of Biological Chemistry

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“…21 To assess the effect of ␤ 1 overexpression on NRVM hypertrophic signaling, a recombinant adenoviral vector was constructed to express the human ␤ 1A integrin subunit. The human ␤ 1 subunit is similar to ␤ 1 integrins from other species, including rat, 45 and dimerization of integrin subunits from divergent species (eg, mouse and chick) has been observed previously. 46 Human ␤ 1 was differentiated from the endogenous rat ␤ 1 with speciesspecific antibodies.…”

Section: ␤ 1 Integrin Overexpression Augments Adrenergic Induction Ofmentioning

confidence: 77%

β ₁ Integrins Participate in the Hypertrophic Response of Rat Ventricular Myocytes

Ross

Pham

Shai

et al. 1998

Circulation Research

173

129

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Abstract-Multiple signaling pathways have been implicated in the hypertrophic response of ventricular myocytes, yet the importance of cell-matrix interactions has not been extensively examined. Integrins are cell-surface molecules that link the extracellular matrix to the cellular cytoskeleton. They can function as cell signaling molecules and transducers of mechanical information in noncardiac cells. Given these properties and their abundance in cardiac cells, we evaluated the hypothesis that ␤ 1 integrin function is involved in the ␣ 1 -adrenergic mediated hypertrophic response of neonatal rat ventricular myocytes. The hypertrophic response of this model required interaction with extracellular matrix proteins. Specificity of these results was confirmed by demonstrating that ventricular myocytes plated onto an anti-␤ 1 integrin antibody supported the hypertrophic gene response. Adenovirus-mediated overexpression of ␤ 1 integrin augmented the myocyte hypertrophic response when assessed by protein synthesis and atrial natriuretic factor production, a marker gene of hypertrophic induction. DNA synthesis was not altered by integrin overexpression. Transfection of cultured cardiac myocytes with either the ubiquitously expressed ␤ 1A integrin or the cardiac/skeletal muscle-specific ␤ 1 isoform (␤ 1D ) activated reporter expression from both the atrial natriuretic factor and myosin light chain-2 ventricular promoters, genetic markers of ventricular cell hypertrophy. Finally, suppression of integrin signaling by overexpression of free ␤ 1 integrin cytoplasmic domains inhibited the adrenergically mediated atrial natriuretic factor response. These findings show that integrin ligation and signaling are involved in the cardiac hypertrophic response pathway. (Circ Res. 1998;82:1160-1172.)Key Words: hypertrophy Ⅲ myocardium Ⅲ extracellular matrix Ⅲ integrin Ⅲ myocyte M echanical stress on the heart from either pressure or volume loading can cause hypertrophy of the myocardium to optimize cardiac performance. Results from in vivo studies and cell culture models have indicated that morphological hypertrophy coincides with a cascade of intracellular biochemical signals.

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“…Transverse sections (10 m) were cut in a cryostat (Microm, Heidelberg, Germany), thawed onto Probe-On slides (Fisher Scientific, Pittsburg, PA), and stored in sealed black boxes at -70°C until use. The synthetic oligonucleotides were complementary to mRNA encoding rat GAP-43 (nucleotides 70 -117; Karns et al, 1987), mouse integrin subunit ␣6 (nucleotides 1, 899 -1,946 or 3,165-3,208;Hierck et al, 1993), rat integrin subunit ␣7 (nucleotides 1,850 -1,899 or 2,301-2,347; Song et al, 1992), and rat integrin subunit ␤1 (nucleotides 822-869 or 2,106 -2,149; Malek-Hedayat and Rome, 1995). The sequences of the probes were run in a GenBank database search to exclude significant homology with other related as well as unrelated genes.…”

Section: In Situ Hybridization Histochemistrymentioning

confidence: 99%

Dorsal root ganglion neurons up‐regulate the expression of laminin‐associated integrins after peripheral but not central axotomy

Wallquist

Zelano

Plantman

et al. 2004

J of Comparative Neurology

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The favorable prognosis of regeneration in the peripheral nervous system after axonal lesions is generally regarded as dependent on the Schwann cell basal lamina. Laminins, a heterotrimeric group of basal lamina molecules, have been suggested to be among the factors playing this supportive role. For neurons to utilize laminin as a substrate for growth, an expression of laminin binding receptors, integrins, is necessary. In this study, we have examined the expression of laminin binding integrin subunits in dorsal root ganglion (DRG) neurons after transection to either their peripherally projecting axons, as in the sciatic nerve, followed by regeneration, or the centrally projecting axons in dorsal roots, followed by no or weak regenerative activity. In uninjured DRG, immunohistochemical staining revealed a few neurons expressing integrin subunit alpha6, whereas integrin subunits alpha7 and foremost beta1 were expressed in a majority of neurons. After an injury to the sciatic nerve, mRNAs encoding all three integrins were up-regulated in DRG neurons. By anterograde tracing, immunoreactivity for all studied integrins was also found in association with growing axons after a sciatic nerve crush lesion in vivo. In contrast, mRNA levels remained constant in DRG neurons after a dorsal root injury. Together with previous findings, this suggests that integrin subunits alpha6, alpha7, and beta1 have an important role in the regenerative response following nerve injury and that the lack of regenerative capacity following dorsal root injury could in part be explained by the absence of response in integrin regulation.

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Cloning and sequence of the cDNA encoding the rat oligodendrocyte integrin β1 subunit

Cited by 15 publications

References 8 publications

Y Receptor-mediated Induction of CD63 Transcripts, a Tetraspanin Determined To Be Necessary for Differentiation of the Intestinal Epithelial Cell Line, hBRIE 380i Cells

Y Receptor-mediated Induction of CD63 Transcripts, a Tetraspanin Determined To Be Necessary for Differentiation of the Intestinal Epithelial Cell Line, hBRIE 380i Cells

β ₁ Integrins Participate in the Hypertrophic Response of Rat Ventricular Myocytes

Dorsal root ganglion neurons up‐regulate the expression of laminin‐associated integrins after peripheral but not central axotomy

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Cloning and sequence of the cDNA encoding the rat oligodendrocyte integrin β1 subunit

Cited by 15 publications

References 8 publications

Y Receptor-mediated Induction of CD63 Transcripts, a Tetraspanin Determined To Be Necessary for Differentiation of the Intestinal Epithelial Cell Line, hBRIE 380i Cells

Y Receptor-mediated Induction of CD63 Transcripts, a Tetraspanin Determined To Be Necessary for Differentiation of the Intestinal Epithelial Cell Line, hBRIE 380i Cells

β 1 Integrins Participate in the Hypertrophic Response of Rat Ventricular Myocytes

Dorsal root ganglion neurons up‐regulate the expression of laminin‐associated integrins after peripheral but not central axotomy

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β ₁ Integrins Participate in the Hypertrophic Response of Rat Ventricular Myocytes