Cloning and Sequence of the L<sub>2</sub>Form of RubisCO from a Marine Obligately Autotrophic Hydrogen-oxidizing Bacterium,<i>Hydrogenovibrio marinus</i>Strain MH-110

Yaguchi, Toshiaki; Chung, Seong Yong; Igarashi, Yasuo; Kodama, Tohru

doi:10.1271/bbb.58.1733

Cited by 27 publications

(20 citation statements)

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“…Interestingly, there are examples of free-living bacteria that express both forms of RubisCO, such as the vent isolate Thiomicrospira L-12 (C. Cavanaugh unpubl. data) and Hydrogenovibrio marinus (Yaguchi et al 1994). Thus, there is the potential for an ancestral symbiont with both Form I and Form II RubisCOs which gave rise to the mussel and clam symbionts with subsequent differential retention of the enzyme.…”

Section: Discussionmentioning

confidence: 96%

The ubiquitous mussel: Bathymodiolus aff. brevior symbiosis at the Central Indian Ridge hydrothermal vents

McKiness¹,

Cavanaugh²

2005

Mar. Ecol. Prog. Ser.

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The discovery and exploration of hydrothermal vents on the Central Indian Ridge (CIR) yielded invaluable samples for further resolution of the biogeography of chemoautotrophic symbioses. Mytilid mussels were collected from 2 CIR hydrothermal vent fields and preliminary molecular analyses suggested phylogenetic affinity with the western Pacific vent mussel Bathymodiolus brevior. Resolving whether this mussel, designated B. aff. brevior, hosts a dual symbiosis, as characterized for the 2 Mid-Atlantic Ridge hydrothermal vent mussel species, or a single chemoautotrophic symbiosis, as seen in the Pacific vent mussel species, will provide insight into the evolutionary history of mytilid symbioses. Ultrastructural, physiological, and molecular evidence are reported herein which support the presence of a single endosymbiont phylotype with chemoautotrophic metabolism. Phylogenetic analyses placed this symbiont in the same clade as the vesicomyid clam symbionts, prompting discussion regarding the evolutionary origin of chemoautotrophic symbioses in vent bivalves.KEY WORDS: Chemoautotrophic endosymbiosis · Central Indian Ridge · Hydrothermal vent · Bathymodiolus spp. Resale or republication not permitted without written consent of the publisher

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Section: Discussionmentioning

confidence: 96%

The ubiquitous mussel: Bathymodiolus aff. brevior symbiosis at the Central Indian Ridge hydrothermal vents

McKiness¹,

Cavanaugh²

2005

Mar. Ecol. Prog. Ser.

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“…We have shown that H. marinus possesses three types of RubisCO: two form I RubisCOs (CbbLS-1 and CbbLS-2), and a form II RubisCO (CbbM) (Fig. 1) (Chung et al, 1993;Yaguchi et al, 1994;Hayashi et al, 1998;Yoshizawa et al, 2004). The ambient CO 2 concentration affects the expression of these three RubisCO genes (Yoshizawa et al, 2004).…”

Section: Introductionmentioning

confidence: 91%

The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus strain MH-110

et al. 2005

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Hydrogenovibrio marinus MH-110 possesses three different sets of genes for ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO): two form I (cbbLS-1 and cbbLS-2) and one form II (cbbM). We have previously shown that the expression of these RubisCO genes is dependent on the ambient CO 2 concentration. LysR-type transcriptional regulators, designated CbbR1 and CbbRm, are encoded upstream of the cbbLS-1 and cbbM genes, respectively. In this study, we revealed by gel shift assay that CbbR1 and CbbRm bind with higher affinity to the promoter regions of cbbLS-1 and cbbM, respectively, and with lower affinity to the other RubisCO gene promoters. The expression patterns of the three RubisCOs in the cbbR1 and the cbbRm gene mutants showed that CbbR1 and CbbRm were required to activate the expression of cbbLS-1 and cbbM, respectively, and that neither CbbR1 nor CbbRm was required for the expression of cbbLS-2. The expression of cbbLS-1 was significantly enhanced under high-CO 2 conditions in the cbbRm mutant, in which the expression of cbbM was decreased. Although cbbLS-2 was not expressed under high-CO 2 conditions in the wild-type strain or the single cbbR mutants, the expression of cbbLS-2 was observed in the cbbR1 cbbRm double mutant, in which the expression of both cbbLS-1 and cbbM was decreased. These results indicate that there is an interactive regulation among the three RubisCO genes.

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“…Furthermore, Halothiobacillus neapolitanus (formerly Thiobacillus neapolitanus), Thiomonas intermedia (formerly Thiobacillus intermedius), Thiobacillus denitrificans, Rhodobacter sphaeroides and Rhodobacter capsulatus have genes for both form I and form II enzymes (Gibson & Tabita, 1977a, b;Shively et al, 1986;English et al, 1992;Stoner & Shively, 1993;Paoli et al, 1995). Acidithiobacillus ferrooxidans (formerly Thiobacillus ferrooxidans) and Hydrogenovibrio marinus have three different sets of RubisCO genes, two encoding the green-like form I enzyme, whereas the third one encodes a form II enzyme (Kusano et al, 1991;Heinhorst et al, 2002;Yaguchi et al, 1994;Nishihara et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Occurrence, phylogeny and evolution of ribulose-1,5-bisphosphate carboxylase/oxygenase genes in obligately chemolithoautotrophic sulfur-oxidizing bacteria of the genera Thiomicrospira and Thioalkalimicrobium

et al. 2006

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The occurrence of the different genes encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), the key enzyme of the Calvin-Benson-Bassham cycle of autotrophic CO 2 fixation, was investigated in the members of the genus Thiomicrospira and the relative genus Thioalkalimicrobium, all obligately chemolithoautotrophic sulfur-oxidizing Gammaproteobacteria. The cbbL gene encoding the 'green-like' form I RubisCO large subunit was found in all analysed species, while the cbbM gene encoding form II RubisCO was present only in Thiomicrospira species. Furthermore, species belonging to the Thiomicrospira crunogena 16S rRNA-based phylogenetic cluster also possessed two genes of green-like form I RubisCO, cbbL-1 and cbbL-2. Both 16S-rRNA-and cbbL-based phylogenies of the Thiomicrospira-Thioalkalimicrobium-Hydrogenovibrio group were congruent, thus supporting its monophyletic origin. On the other hand, it also supports the necessity for taxonomy reorganization of this group into a new family with four genera.

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Cloning and Sequence of the L₂Form of RubisCO from a Marine Obligately Autotrophic Hydrogen-oxidizing Bacterium,Hydrogenovibrio marinusStrain MH-110

Cited by 27 publications

References 8 publications

The ubiquitous mussel: Bathymodiolus aff. brevior symbiosis at the Central Indian Ridge hydrothermal vents

The ubiquitous mussel: Bathymodiolus aff. brevior symbiosis at the Central Indian Ridge hydrothermal vents

The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus strain MH-110

Occurrence, phylogeny and evolution of ribulose-1,5-bisphosphate carboxylase/oxygenase genes in obligately chemolithoautotrophic sulfur-oxidizing bacteria of the genera Thiomicrospira and Thioalkalimicrobium

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Cloning and Sequence of the L2Form of RubisCO from a Marine Obligately Autotrophic Hydrogen-oxidizing Bacterium,Hydrogenovibrio marinusStrain MH-110

Cited by 27 publications

References 8 publications

The ubiquitous mussel: Bathymodiolus aff. brevior symbiosis at the Central Indian Ridge hydrothermal vents

The ubiquitous mussel: Bathymodiolus aff. brevior symbiosis at the Central Indian Ridge hydrothermal vents

The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus strain MH-110

Occurrence, phylogeny and evolution of ribulose-1,5-bisphosphate carboxylase/oxygenase genes in obligately chemolithoautotrophic sulfur-oxidizing bacteria of the genera Thiomicrospira and Thioalkalimicrobium

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Cloning and Sequence of the L₂Form of RubisCO from a Marine Obligately Autotrophic Hydrogen-oxidizing Bacterium,Hydrogenovibrio marinusStrain MH-110