Cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium Sulfolobus acidocaldarius

Maruta, Kazuhiko; Mitsuzumi, Hitoshi; Nakada, Tetsuya; Kubota, Michio; Chaen, Hiroto; Fukuda, Shigeharu; Sugimoto, Toshiyuki; Kurimoto, Masashi

doi:10.1016/s0304-4165(96)00082-7

Cited by 115 publications

(70 citation statements)

References 20 publications

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“…The gene products of all0167 and all0168 show 38 % and 45 % identity to Mts and Mth from Sulfolobus acidocaldarius, respectively, which produce trehalose from the reducing end of a-1,4-glucan by sequential reactions (Maruta et al, 1996). The gene product of all0166 shows 32 % identity to trehalase from Saccharomyces cerevisiae, which hydrolyses trehalose (Kopp et al, 1993).…”

Section: Resultsmentioning

confidence: 99%

The role of a gene cluster for trehalose metabolism in dehydration tolerance of the filamentous cyanobacterium Anabaena sp. PCC 7120

et al. 2006

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Expression of the genes for trehalose synthesis (mts and mth, encoding maltooligosyl trehalose synthase and hydrolase) and trehalose hydrolysis (treH) in Anabaena sp. PCC 7120 was up-regulated markedly upon dehydration. However, the amount of trehalose accumulated during dehydration was small, whereas a large amount of sucrose was accumulated. Northern blotting analysis revealed that these genes were transcribed as an operon. Gene disruption of mth resulted in a decrease in the trehalose level and in tolerance during dehydration. In contrast, gene disruption of treH resulted in an increase in both the amount of trehalose and tolerance. These results suggest that trehalose is important for the dehydration tolerance of this cyanobacterium. The amount of trehalose accumulated during dehydration was small, corresponding to 0?05-0?1 % of dry weight, suggesting that trehalose did not stabilize proteins and membranes directly during dehydration. To reveal the role of trehalose, the expression profiles of the wild-type strain and gene disruptants during dehydration were compared by using oligomeric DNA microarray. It was found that the expression of two genes, one of which encodes a cofactor of a chaperone DnaK, correlated with trehalose content, suggesting that a chaperone system induced by trehalose is important for the dehydration tolerance of Anabaena sp. PCC 7120.

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Section: Resultsmentioning

confidence: 99%

The role of a gene cluster for trehalose metabolism in dehydration tolerance of the filamentous cyanobacterium Anabaena sp. PCC 7120

et al. 2006

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“…This pathway is also found in other bacterial species such as Rhizobium (Maruta et al, 1996a), Bradyrhizobium japonicum (Sugawara et al, 2010) and Corynebacterium (Tzvetkov et al, 2003), but is missing in other major bacterial groups including E. coli and Bacillus subtilis. Archaea Sulfolobus also uses this pathway for trehalose synthesis (Maruta et al, 1996b).…”

Section: Trey-trez Pathwaymentioning

confidence: 99%

Trehalose and Abiotic Stress in Biological Systems

Iordăchescu¹,

Imai²

2011

Abiotic Stress in Plants - Mechanisms and Adaptations

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“…In Sulfolobus acidocaldarius, M. tuberculosis and Arthrobacter sp. Q36 the treY and treZ genes constitute an operon with a third gene designated as treX, which is thought to have a glycogendebranching function in the trehalose biosynthesis process (Maruta et al, 1996c(Maruta et al, , 2000Cole et al, 1998). A possible treX homologue, NCgl2026, was identified in the C. glutamicum genome 10 kb upstream of treY gene (data not shown).…”

Section: Analysis Of C Glutamicum Genome Sequence Datamentioning

confidence: 99%

“…An alternative pathway for trehalose synthesis that uses glycogen as the initial substrate (TreYTreZ pathway; Fig. 1b) was discovered in some bacteria (Maruta et al, 1996a, b) and archaea (Maruta et al, 1996c). In this case, first the terminal a(1R4) glycosidic bond at the reducing end of the a-glucan polymer is transformed into an a(1R1) glycosidic bond via transglycosylation, resulting in the formation of a terminal trehalosyl unit.…”

Section: Introductionmentioning

confidence: 99%

Genetic dissection of trehalose biosynthesis in Corynebacterium glutamicum: inactivation of trehalose production leads to impaired growth and an altered cell wall lipid composition

et al. 2003

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The analysis of the available Corynebacterium genome sequence data led to the proposal of the presence of all three known pathways for trehalose biosynthesis in bacteria, i.e. trehalose synthesis from UDP-glucose and glucose 6-phosphate (OtsA-OtsB pathway), from malto-oligosaccharides or α-1,4-glucans (TreY-TreZ pathway), or from maltose (TreS pathway). Inactivation of only one of the three pathways by chromosomal deletion did not have a severe impact on C. glutamicum growth, while the simultaneous inactivation of the OtsA-OtsB and TreY-TreZ pathway or of all three pathways resulted in the inability of the corresponding mutants to synthesize trehalose and to grow efficiently on various sugar substrates in minimal media. This growth defect was largely reversed by the addition of trehalose to the culture broth. In addition, a possible pathway for glycogen synthesis from ADP-glucose involving glycogen synthase (GlgA) was discovered. C. glutamicum was found to accumulate significant amounts of glycogen when grown under conditions of sugar excess. Insertional inactivation of the chromosomal glgA gene led to the failure of C. glutamicum cells to accumulate glycogen and to the abolition of trehalose production in a ΔotsAB background, demonstrating that trehalose production via the TreY-TreZ pathway is dependent on a functional glycogen biosynthetic route. The trehalose-non-producing mutant with inactivated OtsA-OtsB and TreY-TreZ pathways displayed an altered cell wall lipid composition when grown in minimal broth in the absence of trehalose. Under these conditions, the mutant lacked both major trehalose-containing glycolipids, i.e. trehalose monocorynomycolate and trehalose dicorynomycolate, in its cell wall lipid fraction. The results suggest that a dramatically altered cell wall lipid bilayer of trehalose-less C. glutamicum mutants may be responsible for the observed growth deficiency of such strains in minimal medium. The results of the genetic and physiological dissection of trehalose biosynthesis in C. glutamicum reported here may be of general relevance for the whole phylogenetic group of mycolic-acid-containing coryneform bacteria.

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Cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium Sulfolobus acidocaldarius

Cited by 115 publications

References 20 publications

The role of a gene cluster for trehalose metabolism in dehydration tolerance of the filamentous cyanobacterium Anabaena sp. PCC 7120

The role of a gene cluster for trehalose metabolism in dehydration tolerance of the filamentous cyanobacterium Anabaena sp. PCC 7120

Trehalose and Abiotic Stress in Biological Systems

Genetic dissection of trehalose biosynthesis in Corynebacterium glutamicum: inactivation of trehalose production leads to impaired growth and an altered cell wall lipid composition

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