Trehalose and Abiotic Stress in Biological Systems

Iordăchescu, Mihaela; Imai, Ryozo

doi:10.5772/22208

Cited by 17 publications

(12 citation statements)

References 96 publications

(102 reference statements)

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“…Increases in τ̅ arise because of the sensitivity of the induced dipole of the excited state to solvation and reflect changes in solvent structure. Consistent with our results, solvent reorganization has previously been suggested to underlie the stabilization of the bioactive protein conformation that arises upon addition of crowding agents. ,,, Mechanisms of stabilization include enhancements in hydrogen bonds and other electrostatic interactions that stabilize the native state. Further, favorable attractive interactions between the PEG matrix and the scFv antibody act to stabilize the bioactive protein structure and counteract reductions in conformational entropy arising from excluded volume effects associated with molecular crowding .…”

Section: Discussionsupporting

confidence: 91%

Hydrogel Tethering Enhances Interdomain Stabilization of Single-Chain Antibodies

et al. 2017

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Here, we identify the importance of molecular crowding agents in the functional stabilization of scFv antibodies. Antibodies were tethered through an engineered calmodulin (CaM)-binding peptide into a stimulus-responsive hydrogel composed of poly(ethylene glycol) (PEG)-functionalized CaM. Macromolecular crowding is modulated by transient heating, which decreases effective pore sizes. Using a fluorescent ligand bound to the scFv, frequency-domain fluorescence spectroscopy was used to assess the structural coupling between the V and the V domains and relationships with functional stabilization. There is minimal structural coupling between the V and the V domains in solution, as is apparent from the substantial rotational mobility for the bound ligand, that is suggestive of an independent mobility for the V and the V domains. In comparison, the hydrogel matrix acts to structurally couple the V and the V domains, resulting in a reduction in rotational mobility and a retention of ligand binding in the presence of 8.0 M urea. Under these same conditions, ligand binding is disrupted for scFv antibodies in solution. Increases in the stabilization of scFv antibodies in hydrogels is not simply the result of molecular crowding because decreases in pore size act to destabilize ligand binding. Rather, our results suggest that the functional stabilization of the scFv antibody within the PEG hydrogel matrix includes important factors involving protein solvation that stabilize interdomain interactions between the V and the V domains necessary for ligand binding.

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Section: Discussionsupporting

confidence: 91%

Hydrogel Tethering Enhances Interdomain Stabilization of Single-Chain Antibodies

et al. 2017

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“…According to Da Costa Morato Nery et al (2008) this mechanism is involved in minimizing the oxidative damage caused to both proteins and lipids, which would require the presence of trehalose on both sides of the lipid bilayer. There are data for up-regulation of the genes, responsible for trehalose-and glycogen-metabolizing enzymes, resulting in their accumulation under conditions of cold stress (Aguilera et al 2007;Kostadinova et al 2009;Iordachescu andImai 2011). Interestingly, Tsuji (2016) reported that two strains of the Antarctic Mrakia blollopis demonstrate difference in cold stress response.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

Kostadinova¹,

Tosi²,

Spassova³

et al. 2017

Polish Polar Research

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Two fungal strains, isolated from Livingston Island, Antarctica (Penicillium commune 161, psychrotolerant and Aspergillus glaucus 363, mesophilic) were investigated for a relationship between growth temperature and oxidative stress response. Cultivation at temperatures below -(10 and 15°C and 10 and 20°C for P. commune and A. glaucus, respectively) and above (25°C and 30°C for P. commune and A. glaucus, respectively) the optimum caused significant difference in growth and glucose uptake in comparison with the control cultures. Enhanced level of reserve carbohydrates (glycogen and trehalose) was determined under cultivation at different temperatures from the optimal one. While the highest content of trehalose was found in the exponential phase, glycogen accumulation was observed in the stationary phase when growth conditions deteriorate. The growth at temperature below-and above-optimum caused strain-dependent changes in two antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). While SOD activity in the psychrotolerant strain increases with decreasing of growth temperature, the mesophilic A. glaucus demonstrated marked reduction of it at below-and above-optimal temperature. Decreasing trend of CAT activity was observed in both strains below the optimal temperature indicating a lack of antioxidant protection from this enzyme under the cold stress conditions.

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“…Trehalose is a safe, non-reducing sugar that contains two glucose molecules and stores energy for use under stress conditions. Microbes can accelerate trehalose biosynthesis through the TPS/trehalose-6-phosphate phosphatases (TPS/TPP) pathway, maintain osmolytes concentrations, and stabilize turgor pressure in plant cells ( Iordachescu and Imai, 2011 ; Kahraman et al, 2019 ). The application of microbes to plant cells increases the secretion of organic acids (e.g., oxalic acid, citric acid, and malic acid) and minerals (e.g., chlorine, sodium, and potassium), which are important for nutrient availability, metabolic reactions, and the maintenance of osmoregulation in plant cells ( Kirst, 1996 ; Chen and Jiang, 2010 ).…”

Section: Molecular Mechanism Of Action Of Microbes In Plants Under Dr...mentioning

confidence: 99%

Research Progress in the Field of Microbial Mitigation of Drought Stress in Plants

et al. 2022

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Plants defend themselves against ecological stresses including drought. Therefore, they adopt various strategies to cope with stress, such as seepage and drought tolerance mechanisms, which allow plant development under drought conditions. There is evidence that microbes play a role in plant drought tolerance. In this study, we presented a review of the literature describing the initiation of drought tolerance mediated by plant inoculation with fungi, bacteria, viruses, and several bacterial elements, as well as the plant transduction pathways identified via archetypal functional or morphological annotations and contemporary “omics” technologies. Overall, microbial associations play a potential role in mediating plant protection responses to drought, which is an important factor for agricultural manufacturing systems that are affected by fluctuating climate.

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Trehalose and Abiotic Stress in Biological Systems

Cited by 17 publications

References 96 publications

Hydrogel Tethering Enhances Interdomain Stabilization of Single-Chain Antibodies

Hydrogel Tethering Enhances Interdomain Stabilization of Single-Chain Antibodies

Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

Research Progress in the Field of Microbial Mitigation of Drought Stress in Plants

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