Cloning and Tissue Expression of Chicken Heart Fatty Acid-Binding Protein and Intestine Fatty Acid-Binding Protein Genes

Wang, Qigui; Li, Hui; Liu, Shuang; Wang, Guihua; Wang, Yuxiang

doi:10.1080/10495390500276882

Cited by 22 publications

(9 citation statements)

References 16 publications

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“…Fatty‐acid binding proteins (FABP) are found in the animal intestines, liver, heart, skeletal muscle and other tissues. These proteins are involved in fatty‐acid metabolism, including absorption, transportation and regulation of the concentration gradient across cellular membranes (Wang et al , 2005). Intestinal FABP (FABP2) affects animal growth and development.…”

Section: Introductionmentioning

confidence: 99%

Identification and expression analysis of fabp2 gene from common carp Cyprinus carpio

Chen

Jiang

2012

Journal of Fish Biology

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Two complementary (c)DNA fragments, including the complete open reading frame of fabp2 from the common carp Cyprinus carpio, were cloned by reverse-transcription polymerase chain reaction (RT-PCR). Both were putative intestinal-type fabp genes, named fabp2a and fabp2b. fabp2b was mainly expressed in the intestine and the brain. This gene, however, was nearly not expressed in the liver, heart, pancreas and muscle. fabp2a was only expressed at a very low level in the intestine. Western blot also showed that Fabp2 is relatively highly expressed in the intestine and the brain. Immunohistochemical analysis revealed that Fabp2 is widely distributed in the mucosa of the intestine. These findings provide novel insights into the fabp2 gene molecular evolution, as well as its potential features in the intestine and the brain.

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Section: Introductionmentioning

confidence: 99%

Identification and expression analysis of fabp2 gene from common carp Cyprinus carpio

Chen

Jiang

2012

Journal of Fish Biology

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“…The low molecular weight protein is predominantly detectable in myocardial cytoplasm and lung tissue. It participates in the uptake and transport of long chain fatty acids and is suggested as a promising biomarker of cardiac impairment [98][99][100] . Recent studies indicate, that hFABP can independently predict 28-d mortality and organ failure in patients with sepsis, severe sepsis or septic shock.…”

Section: Heart Type Fatty Acid Binding Proteinmentioning

confidence: 99%

Are there new approaches for diagnosis, therapy guidance and outcome prediction of sepsis?

Kojic

Siegler

Uhle

et al. 2015

WJEM

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Beside many efforts to improve outcome, sepsis is still one of the most frequent causes of death in critically ill patients. It is the most common condition with high mortality in intensive care units. The complexity of the septic syndrome comprises immunological aspects -i.e. , sepsis induced immunosuppression -but is not restricted to this fact in modern concepts. So far, exact mechanisms and variables determining outcome and mortality stay unclear. Since there is no typical risk profile, early diagnosis and risk stratification remain difficult, which hinders rapid and effective treatment initiation. Due to the heterogeneous nature of sepsis, potential therapy options should be adapted to the individual. Biomarkers like C-reactive protein and procalcitonin are routinely used as complementary tools in clinical decision-making. Beyond the acute phase proteins, a wide bunch of promising substances and non-laboratory tools with potential diagnostic and prognostic value is under intensive investigation. So far, clinical decision just based on biomarker assessment is not yet feasible. However, biomarkers should be considered as a complementary approach. Core tip: Sepsis is a complex continuum of disturbed systems. Despite the presence of clinical consensus criteria, the early diagnosis especially in the perioperative setting is challenging. A magnitude of potential new biomarkers is tested for this purpose, but evidence is mounting that due to the complex nature of the syndrome, biomarkers are rather complementary tools for clinical decision making than "magic bullets". Moreover, biomarkers are also evaluated for therapy guidance, linking diagnostic results to an individual therapeutic regime. This review summarizes the developments in the biomarker field, aiming to provide an overview about current targets and their limitations.

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“…These results are consistent with the findings of the present study. However, Wang et al (2005) found that the FABP2 gene was expressed only in intestinal tissues in chickens. The same expression profile was observed in goat (Wang et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis and tissue-specific expression of intestinal-type fatty acid binding protein in domestic pigeons (Columba livia)

Mao

Liu

et al. 2020

Journal of Applied Animal Research

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Intestinal fatty-acid binding protein (I-FABP, also called FABP2) is involved in the uptake of dietary fatty acids in the intestine, and its binding specificity is only with long-chain fatty acid. In this study, sequences of 4 exons in pigeon FABP2 were successfully amplified. The encoded protein had a molecular weight of 15.13 kDa and the theoretical pI was 6.75. Bioinformatics analysis indicated that the protein was predicted to contain 10 potential phosphorylation sites and did not contain signal peptide structure. The evolutionary analysis suggested the amino acid sequence of pigeon FABP2 was closest to the mallard (Nippoia nippon) homologs. Meanwhile the expression of FABP2 mRNA in different ages and tissues we conducted. It showed that the expression of the FABP2 gene was the highest in the liver, followed by the small intestine and adipose tissue, while lowest in the breast muscle. Also, the expression level in the gizzard was gradually increased with aging. Our results may serve as reference to better studies of the functional characterization and structure of the FABP2 in pigeon.

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Cloning and Tissue Expression of Chicken Heart Fatty Acid-Binding Protein and Intestine Fatty Acid-Binding Protein Genes

Cited by 22 publications

References 16 publications

Identification and expression analysis of fabp2 gene from common carp Cyprinus carpio

Identification and expression analysis of fabp2 gene from common carp Cyprinus carpio

Are there new approaches for diagnosis, therapy guidance and outcome prediction of sepsis?

Bioinformatics analysis and tissue-specific expression of intestinal-type fatty acid binding protein in domestic pigeons (Columba livia)

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