Cloning and transcriptional expression of a novel gene during sex inversion of the rice field eel (Monopterus albus)

Qu, Xiancheng; Jiang, Jiaoyun; Cheng, Cui; Feng, Lei; Liu, Q. G.

doi:10.1186/s40064-015-1544-z

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…Finally, the structure of the ovary disappears completely, and the structure of the testis forms afterwards. To date, many differentially expressed genes have been identified and the function was studied on sex reversal in M. albus [ 2 – 7 ], but the mechanism involved in sex reversal is unclear.…”

Section: Introductionmentioning

confidence: 99%

Regulatory mechanism of LncRNAs in gonadal differentiation of hermaphroditic fish, Monopterus albus

Hu,

Xia,

Lian

et al. 2023

Biol Sex Differ

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Background Monopterusalbus is a hermaphroditic fish with sex reversal from ovaries to testes via the ovotestes in the process of gonadal development, but the molecular mechanism of the sex reversal was unknown. Methods We produced transcriptomes containing mRNAs and lncRNAs in the crucial stages of the gonad, including the ovary, ovotestis and testis. The expression of the crucial lncRNAs and their target genes was detected using qRT‒PCR and in situ hybridization. The methylation level and activity of the lncRNA promoter were analysed by applying bisulfite sequencing PCR and dual-luciferase reporter assays, respectively. Results This effort revealed that gonadal development was a dynamic expression change. Regulatory networks of lncRNAs and their target genes were constructed through integrated analysis of lncRNA and mRNA data. The expression and DNA methylation of the lncRNAs MSTRG.38036 and MSTRG.12998 and their target genes Psmβ8 and Ptk2β were detected in developing gonads and sex reversal gonads. The results showed that lncRNAs and their target genes exhibited consistent expression profiles and that the DNA methylation levels were negatively regulated lncRNA expression. Furthermore, we found that Ptk2β probably regulates cyp19a1 expression via the Ptk2β/EGFR/STAT3 pathway to reprogram sex differentiation. Conclusions This study provides novel insight from lncRNA to explore the potential molecular mechanism by which DNA methylation regulates lncRNA expression to facilitate target gene transcription to reprogram sex differentiation in M.albus, which will also enrich the sex differentiation mechanism of teleosts.

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Section: Introductionmentioning

confidence: 99%

Regulatory mechanism of LncRNAs in gonadal differentiation of hermaphroditic fish, Monopterus albus

Hu,

Xia,

Lian

et al. 2023

Biol Sex Differ

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“…Previously, the sex change in M. albus was found to be accompanied by changes in steroid levels ( Yeung and Chan, 1987 ) and activity of enzymes involved in steroid pathways ( Tang et al, 1975 ). Recently, some sex-related genes and microRNAs have been characterized, and their expressions seem to be varied among three genders during the sex-change progress, such as Dmrt ( Sheng et al, 2014 ), AMH ( Hu et al, 2015 ), Foxl2 ( Hu et al, 2014 ) , dax1 ( Hu et al, 2015 ), p450 ( Zhang et al, 2008 ), G2 ( Qu et al, 2015 ), Cyp19a1a ( Liu et al, 2009 ; Zhang et al, 2013 ), gonadal soma-derived factor (gsdf) ( Zhu et al, 2016 ), Sox9 ( Zhou et al, 2003 ), Dmrt1 ( Huang et al, 2005 ), Jnk1 ( Xiao et al, 2010 ), and mal-miR-430a, mal-miR-430b, and mal-miR-430c ( Gao et al, 2014 ; Zhang et al, 2021 ). Moreover, a batch number of genes with different expression profiles between females and males was characterized through high-throughput RNA-seq ( Chi et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Chromosome-Scale, Haplotype-Resolved Genome Assembly of Non-Sex-Reversal Females of Swamp Eel Using High-Fidelity Long Reads and Hi-C Data

Tian

et al. 2022

Front. Genet.

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The Asian swamp eel (Monopterus albus) is an excellent model species for studying sex change and chromosome evolution. M. albus is also widely reared in East Asia and South-East Asia because of its great nutritional value. The low fecundity of this species (about 300 eggs per fish) greatly hinders fries production and breeding programs. Interestingly, about 3–5% of the eels could remain as females for 3 years and lay more than 3,000 eggs per fish, which are referred to as non-sex-reversal (NSR) females. Here, we presented a new chromosome-level genome assembly of such NSR females using Illumina, HiFi, and Hi-C sequencing technologies. The new assembly (Mal.V2_NSR) is 838.39 Mb in length, and the N50 of the contigs is 49.8 Mb. Compared with the previous assembly obtained using the continuous long-read sequencing technology (Mal.V1_CLR), we found a remarkable increase of continuity in the new assembly Mal.V2_NSR with a 20-times longer contig N50. Chromosomes 2 and 12 were assembled into a single contig, respectively. Meanwhile, two highly contiguous haplotype assemblies were also obtained, with contig N50 being 14.54 and 12.13 Mb, respectively. BUSCO and Merqury analyses indicate completeness and high accuracy of these three assemblies. A comparative genomic analysis revealed substantial structural variations (SVs) between Mal.V2_NSR and Mal.V1_CLR and two phased haplotype assemblies, as well as whole chromosome fusion events when compared with the zig-zag eel. Additionally, our newly obtained assembly provides a genomic view of sex-related genes and a complete landscape of the MHC genes. Therefore, these high-quality genome assemblies would provide great help for future breeding works of the swamp eel, and it is a valuable new reference for genetic and genomic studies of this species.

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“…Several genes related to sex determination and differentiation have been identified in M. albus , including cyp19a1a (29), sox9a (30), cyp17 (31), sox17 (32), dmrt1 (33), jnk1 (34), foxl2 (35), miRNAs (36) and gonadal soma-derived factor (37). We also investigated the transcription profiles of some genes involved in gonad development and sex reversal in the M. albus (38, 39). Moreover, a chromosome-scale assembly of M. albus genome is currently available (40).…”

Section: Introductionmentioning

confidence: 99%

Androgen-dmrt1positive feedback programs the rice field eel (Monopterus albus) sex transdifferentiation

Wen

Pan

et al. 2019

Preprint

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17The rice field eel Monopterus albus is a hermaphroditic protogynous fish species that 18 undergoes sex reversal from female to male. However, the potential mechanisms 19 underlying the process of sex transformation are still unclear. We analyzed and 20 compared the gene sequence of M. albus dmrt1 5′ upstream region and its potential 21 transcription factor binding sites with other known species and examined the in vitro 22 34 Androgens are synthesized in large amounts during sex reversal, leading to the 35 promotion of dmrt1 transcription, and thus, gonadal somatic cells transdifferetiation. 36 Overall, androgen-dmrt1 positive feedback programs the M. albus sex reversal. 37 38 39 111 other one on the antisense strand (-903 bp ~ -917 bp) (Supplementary Table 2). 112Histological change 113 After 6 hours of tissue culture, cells began to migrate from the periphery of the 114 130 Ozols' region, aromatic regions and heme-binding region ( Supplementary Fig. 3). We 131 compared the amino acid sequence of M. albus 11β-h to that in other species and 132 found 77% identity with Dicentrarchus labrax, 76% identity with Micropogonias 133 undulatus and 75% identity with Parajulis poecilepterus and Odontesthes bonariensis. 134 The phylogenetic tree analysis showed that the 11β-h of M. albus and Epinephelus 135 coioides, P. poecilepterus, D. labrax, M. undulatus, O. bonariensis, O. latipes and O. 136

show abstract

Cloning and transcriptional expression of a novel gene during sex inversion of the rice field eel (Monopterus albus)

Cited by 7 publications

References 38 publications

Regulatory mechanism of LncRNAs in gonadal differentiation of hermaphroditic fish, Monopterus albus

Regulatory mechanism of LncRNAs in gonadal differentiation of hermaphroditic fish, Monopterus albus

Chromosome-Scale, Haplotype-Resolved Genome Assembly of Non-Sex-Reversal Females of Swamp Eel Using High-Fidelity Long Reads and Hi-C Data

Androgen-dmrt1positive feedback programs the rice field eel (Monopterus albus) sex transdifferentiation

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