Cloning, characterization and expression analysis of SIMP (source of immunodominant MHC-associated peptides) in grass carp Ctenopharyngodon idella

Molecular cloning, characterization and expression patterns of HSP60 in the grass carp (Ctenopharyngodon idella)

“…The first-strand cDNA was subsequently used as the template for PCR. Beta-actin served as an internal control for cDNA normalization [23]. The qRT-PCR mixture consisted of 50 ng of cDNA sample, 7 ml nuclease-free water, 10 ml of 2Â SYBR Premix Ex taqÔ (TaKaRa, Japan), 0.4 ml of ROX Reference Dye, and 0.8 ml of each gene-specific primer (10 mM).…”