Cloning, Characterization and Expression Analysis of Interleukin-10 from the Zebrafish (Danio rerion)

Zhang, Dianchang; Shao, Yi; Huang, Yizhe; Jiang, Shigui

doi:10.5483/bmbrep.2005.38.5.571

Cited by 85 publications

(52 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The up-regulation of IL-10 was as high as 124 and 87 times in spleen and up to 12 and 18 times in head kidney relative to unstimulated control fish at 4 and 8 hpi, respectively. This corresponds to other studies indicating an extensive up-regulation of IL-10 expression in a number of fishes after LPS stimulation (Savan et al 2003, Inoue et al 2005, Zhang et al 2005. In mammals, IL-10 can act as a cytokine inhibitory factor and demonstrates immune-suppressive functions that could minimize damage to the host tissue induced by response to a pathogen (Hummelshoj et al 2006).…”

Section: Discussionmentioning

confidence: 87%

Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination

Raida¹,

Buchmann²

2007

Dis. Aquat. Org.

111

View full text Add to dashboard Cite

The gene expression of immune-relevant genes in rainbow trout Oncorhynchus mykiss following vaccination with a bacterin of Yersinia ruckeri, a bacterial pathogen causing enteric red mouth disease (ERM), was investigated at 5, 15, and 25°C. Rainbow trout were immunized by i.p. injection of a water-based Y. ruckeri (serotype O1) bacterin, and gene expression profiles were compared to control groups injected with phosphate buffered saline (PBS). Blood and tissue samples (spleen and head kidney) were taken for subsequent analysis using solid phase enzyme-linked immunosorbent assay (ELISA) and real-time PCR, respectively. The up-regulation of cytokine genes was generally faster and higher at high water temperature, with major expression at 25°C. The proinflammatory cytokine interleukin (IL)-1β and interferon (IFN)-γ were significantly up-regulated in all immunized groups, whereas the cytokine IL-10 was only up-regulated in fish kept at 15 and 25°C. The gene encoding the C5a (anaphylatoxin) receptor was expressed at a significantly increased level in both head kidney and spleen of immunized fish. The secreted immunoglobulin M (IgM)-encoding gene was significantly up-regulated in the head kidney of immunized trout reared at 25°C, and a positive correlation (r = 0.663) was found between gene expression of secreted IgM in the head kidney and Y. ruckeri-specific antibodies in plasma measured by ELISA. However, no regulation of the teleost specific immunoglobulin T (IgT), which was generally expressed at a much lower level than IgM, could be detected. The study indicated that expression of both innate and specific adaptive immune-response genes are highly temperature-dependent in rainbow trout.

show abstract

Section: Discussionmentioning

confidence: 87%

Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination

Raida¹,

Buchmann²

2007

Dis. Aquat. Org.

111

View full text Add to dashboard Cite

show abstract

“…Sequences coding for il10 homologs in teleosts were described for several fish species (18)(19)(20)(21)(22)(23)(24)(25)(26), including rainbow trout (Oncorhynchus mykiss), goldfish (Carassius auratus), zebrafish (Danio rerio), and common carp (Cyprinus carpio). In most fish species, il10 is primarily expressed in head kidney (bone marrow equivalent), spleen, and gills and is upregulated upon administration of LPS (20,23,25,27). Recombinant goldfish Il10 was shown to reduce radical production in monocytes and inhibit transcription of specific proinflammatory cytokines (23).…”

mentioning

confidence: 99%

Carp Il10 Has Anti-Inflammatory Activities on Phagocytes, Promotes Proliferation of Memory T Cells, and Regulates B Cell Differentiation and Antibody Secretion

Piazzon

Savelkoul

Pietretti

et al. 2015

The Journal of Immunology

110

View full text Add to dashboard Cite

In the current study, we investigated the effects of carp Il10 on phagocytes and lymphocytes. Carp Il10 shares several prototypical inhibitory activities on phagocytes with mammalian IL-10, including deactivation of neutrophils and macrophages, as shown by inhibition of oxygen and nitrogen radical production, as well as reduced expression of proinflammatory genes and mhc genes involved in Ag presentation. Similar to mammalian IL-10, carp Il10 acts through a signaling pathway involving phosphorylation of Stat3, ultimately leading to the early upregulation of socs3 expression. To our knowledge, this is the first study of the effects of Il10 on lymphocytes in fish. Although Il10 did not affect survival and proliferation of T cells from naive animals, it greatly promoted survival and proliferation of T cells in cultures from immunized animals, but only when used in combination with the immunizing Ag. Preliminary gene expression analysis suggests that, under these circumstances, carp Il10 stimulates a subset of CD8+ memory T cells while downregulating CD4+ memory Th1 and Th2 responses. In addition to the regulatory effect on T cells, carp Il10 stimulates proliferation, differentiation, and Ab secretion by IgM+ B cells. Overall, carp Il10 shares several prototypical activities with mammalian IL-10, including downregulation of the inflammatory response of phagocytes, stimulation of proliferation of subsets of memory T lymphocytes, and proliferation, differentiation, and Ab secretion by IgM+ B lymphocytes. To our knowledge, this is the first comprehensive analysis of biological activities of fish Il10 on both phagocytes and lymphocytes showing functional conservation of several properties of Il10.

show abstract

“…It plays an important role in inflammation by inhibiting some activities of macrophages and certain cytokine production. The IL-10 gene has been cloned in some teleosts, including sea bass (Savan et al 2003;Zou et al 2003;Inoue et al 2005;Zhang et al 2005;Buonocore et al 2007a;Pinto et al 2007). From these studies it has emerged that IL-10 in fish is an a-helical polypeptide with a deduced molecular size of 18 kDa, derived from a gene composed of 5 exons and with significant structural similarities with its homologes in mammals (Zou et al 2003).…”

Section: Il-10mentioning

confidence: 99%

An “immunome” gene panel for transcriptomic analysis of immune defence activities in the teleost sea bass (Dicentrarchus labraxL.): a review

Randelli

Buonocore

Casani

et al. 2009

Italian Journal of Zoology

View full text Add to dashboard Cite

Teleost fish are the oldest living vertebrates displaying a morphological and functional immune system conserved until mammals, thus representing a useful model for evolutionary studies. Teleosts are increasingly employed as experimental animal species for studies on physiological activities of vertebrates, and are widely used in the aquaculture industry. Due to its importance as high-quality food, the sea bass is, at present, one of the most important farmed species in the Mediterranean sea and many studies reported on the morphological and functional organisation of immune defences with the aim of controlling its health in farming conditions. From these studies, a panel of genes coding for the main immunomodulatory peptides of innate and acquired defences is available and constituted, at present, by genes coding for innate defences, for acquired defences, and for other immune-related activities. In this review all these genes are collected and defined as the sea bass ''immunome'', that is analysed in an evolutionary context.

show abstract

Cloning, Characterization and Expression Analysis of Interleukin-10 from the Zebrafish (Danio rerion)

Cited by 85 publications

References 21 publications

Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination

Temperature-dependent expression of immune-relevant genes in rainbow trout following Yersinia ruckeri vaccination

Carp Il10 Has Anti-Inflammatory Activities on Phagocytes, Promotes Proliferation of Memory T Cells, and Regulates B Cell Differentiation and Antibody Secretion

An “immunome” gene panel for transcriptomic analysis of immune defence activities in the teleost sea bass (Dicentrarchus labraxL.): a review

Contact Info

Product

Resources

About