Cloning, characterization and expression of the D2 dopamine receptor from the tilapia pituitary

Levavi‐Sivan, Berta; Aizen, Joseph; Avitan, Ayelet

doi:10.1016/j.mce.2005.03.010

Cited by 49 publications

(44 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pit1, GHF-1 and Ptx1 are pituitary-specific transcription factors that modulate the expression of pituitary hormones (Martinez-Barberá et al 1997;Melamed et al 2002). These transcription factors may be relevant to the reported pituitary expression of D 2 R (Vacher et al 2003;Levavi-Sivan et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The muD 2 R cDNA was isolated by Levavi-Sivan et al (2005;AY673984) and its 5¢-UTR sequence (DQ277008) by our laboratory. Following the BD Biosciences (Palo Alto, CA) universal genome walking PCR protocol, the partial intronic sequence 5¢ flanking the first coding exon was isolated from genomic DNA (StuI library) using gene-specific reverse primers (5¢-GACGATGACAAAGATGAGC AGAGTGAGC-3¢ and 5¢-AGTTGTAAGGGTGCTT TTGCTCCCGACC-3¢ for the primary and nested reactions, respectively) and the kit's universal forward primer.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Isolation of dopamine D2 receptor (D2R) promoters in Mugil cephalus

Nocillado

Levavi‐Sivan

Avitan

et al. 2005

Fish Physiol Biochem

View full text Add to dashboard Cite

This paper reports the isolation of two putative D(2)R promoters from grey mullet, one 5'flanking and the other an intronic sequence immediately upstream of the first coding exon. Promoter activity of the intronic sequence was confirmed in vitro through functional analysis using luciferase as reporter gene. The functional characteristics of the region flanking the 5'UTR is currently under investigation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Isolation of dopamine D2 receptor (D2R) promoters in Mugil cephalus

Nocillado

Levavi‐Sivan

Avitan

et al. 2005

Fish Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Constructs were verified by sequencing. Transient transfection, cell procedures and stimulation protocols were according to Levavi-Sivan et al (2005). Briefly, COS-7 cells were grown in DMEM supplemented with 10% FBS, 1% glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin (Biological Industries, Israel) under 5% CO2 until confluent.…”

Section: In Vitro Functional Analysis Of Sbtkiss2r and Ytkkiss2rmentioning

confidence: 99%

The Kiss2 receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii and in Yellowtail Kingfish, Seriola lalandi – Functional analysis and isolation of transcript variants

Nocillado

Biran

Lee

et al. 2012

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

“…In non-mammalian vertebrates, besides D 1 and D 5 receptors (Frail et al 1993;Sugamori et al 1994;Demchyshyn et al 1995;Macrae and Brenner 1995;Cardinaud et al 1997;Hirano et al 1998), unique dopamine D 1 -like receptors, such as D 1C in amphibians and fish (Sugamori et al 1994;Macrae and Brenner 1995;Lamers et al 1996;Cardinaud et al 1997;Hirano et al 1998), and D 1D in birds (Demchyshyn et al 1995), have been identified. The presence of a D 2 receptor subtype has been shown in birds, amphibians and fish (Martens et al 1991;Demchyshyn et al 1995;Macrae and Brenner 1995;Hirano et al 1998;Schnell et al 1999;Levavi-Sivan et al 2005), D 3 in fish (Macrae and Brenner 1995;Hirano et al 1998;Boehmler et al 2004;Pasqualini et al 2009), and D 4 in birds and fish (Hirano et al 1998;Sugiyama et al 2004;Boehmler et al 2007;Fidler et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Kinetics and Pharmacology of the D1- and D2-Like Dopamine Receptors in Japanese Quail Brain

2009

View full text Add to dashboard Cite

Although the avian brain dopamine system and its functions have been studied much less than the mammalian one, there is an increasing interest in the role of dopamine and its receptors in a wide variety of motor, cognitive and emotional functions in birds with implications for basic research, medicine or agriculture. Pharmacological characterisation of the avian dopamine receptors has had little attention. In this paper we characterise the two classes of dopamine receptors in Japanese quail brain by radioligand binding techniques using [(3)H]SCH 23390 (D(1)) and [(3)H]spiperone (D(2)). Association, dissociation and saturation analyses showed that the binding of both radioligands is time- and concentration-dependent, saturable and reversible. Apparent dissociation constants determined for [(3)H]SCH 23390 and [(3)H]spiperone from concentration isotherms were 1.07 and 0.302 nM and the maximum binding capacities were 89.3 and 389.3 fmol per mg of protein, respectively. Using competitive binding studies with a spectrum of dopamine and other neurotransmitter receptor agonists/antagonists, the [(3)H]SCH 23390 and [(3)H]spiperone binding sites were characterised pharmacologically. Pharmacological profiles of quail dopamine receptors showed a high degree of pharmacological homology with other vertebrate dopamine receptors. The data presented extend the knowledge of kinetics and pharmacology of D(1)- and D(2)-like dopamine receptors in birds, provide data for avian psychopharmacological and comparative studies and represent an important complement to studies using cell expression systems.

show abstract

Cloning, characterization and expression of the D2 dopamine receptor from the tilapia pituitary

Cited by 49 publications

References 65 publications

Isolation of dopamine D2 receptor (D2R) promoters in Mugil cephalus

Isolation of dopamine D2 receptor (D2R) promoters in Mugil cephalus

The Kiss2 receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii and in Yellowtail Kingfish, Seriola lalandi – Functional analysis and isolation of transcript variants

Kinetics and Pharmacology of the D1- and D2-Like Dopamine Receptors in Japanese Quail Brain

Contact Info

Product

Resources

About