Cloning, DNA nucleotide sequence and distribution of the gene encoding the SEF14 fimbrial antigen of Salmonella entevitidis

Turcotte, Claude; Woodward, Martin J.

doi:10.1099/00221287-139-7-1477

Cited by 61 publications

(44 citation statements)

References 15 publications

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“…In addition to the fimbriae that are shared between serovars, Salmonella Enteritidis contains fimbriae such as the Salmonella Enteritidis SEF14 fimbriae that are not found in Salmonella Typhimurium. The SEF14 fimbriae require four proteins for biogenesis : the major subunit (SefA), chaperone (SefB) and usher (SefC) have previously been characterized (Clouthier et al, 1993 ;Thorns et al, 1990 ;Turcotte & Woodward, 1993). The minor subunit (SefD) was also previously identified but not thought to be part of the SEF14 fimbriae (Clouthier et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Genomic analysis and growth-phase-dependent regulation of the SEF14 fimbriae of Salmonella enterica serovar Enteritidis The GenBank accession number for the sequence reported in this paper is AF239978.

et al. 2001

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Salmonella enterica serovar Enteritidis is a leading cause of food poisoning in the USA and Europe. Although Salmonella serovars share many fimbrial operons, a few fimbriae are limited to specific Samonella serovars. SEF14 fimbriae are restricted to group D Salmonella and the genes encoding this virulence factor were acquired relatively recently. Genomic, genetic and gene expression studies have been integrated to investigate the ancestry, regulation and expression of the sef genes. Genomic comparisons of the Salmonella serovars sequenced revealed that the sef operon is inserted in leuX in Salmonella Enteritidis, Salmonella Paratyphi and Salmonella Typhi, and revealed the presence of a previously unidentified 25 kb pathogenicity island in Salmonella Typhimurium at this location. Salmonella Enteritidis contains a region of homology between the Salmonella virulence plasmid and the chromosome downstream of the sef operon. The sef operon itself consists of four co-transcribed genes, sefABCD, and adjacent to sefD there is an AraC-like transcriptional activator that is required for expression of the sef genes. Expression of the sef genes was optimal during growth in late exponential phase and was repressed during stationary phase. The regulation was coordinated by the RpoS sigma factor.

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Section: Introductionmentioning

confidence: 99%

Genomic analysis and growth-phase-dependent regulation of the SEF14 fimbriae of Salmonella enterica serovar Enteritidis The GenBank accession number for the sequence reported in this paper is AF239978.

et al. 2001

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“…The SEF17 fimbrial structure, which was demonstrated to share 70°% identity with the 'curli' fimbrial structure of E. coli [20], generated an aggregative phenotype and bound fibronectin [21][22][23]. The SEF14 fimbrial structure [24][25][26][27] was implicated in persistent infection in the chicken [28,29]. The genetic determinants of the SEF'D' fimbrial structure, also referred to as SEF18, were colocated with the genes encoding SEF14 [30] but as yet no demonstrable phenotype has been ascribed to the SEF'D' fimbrial structure.…”

Section: Introductionmentioning

confidence: 99%

Distribution, gene sequence and expressionin vivoof the plasmid encoded fimbrial antigen ofSalmonellaserotype Enteritidis

1996

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SUMMARYThe pefA gene which encoded the serotype associated plasmid (SAP) screened by hybridization for the presence of pef sequences. Recombinant clones which were deduced to harbour the entire pef operon elaborated a PEF-like fimbrial structure at the cell surface. The PEF-like fimbrial antigen was purified from one cosmid clone and used in western blot experiments with sera from chickens infected with Enteritidis phage-type 4. Seroconversion to the fimbrial antigen was observed which indicated that the Enteritidis PEF-like fimbrial structure was expressed at some stage during infection. Nucleotide sequence analysis demonstrated that the pefA alleles of Typhimurium and Enteritidis phage-type 4 shared 76 % DNA nucleotide and 82 % deduced amino acid sequence identity.

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“…Both carry fimbrial operons including the one for SEF14 fimbriae which may be important in colonization of reproductive tissue and eggs during egg formation (Turcotte and Woodward, 1993). Although S. Enteritidis and S. Gallinarum share similarities in pathogenesis and pathobiology of infection, they are different in terms of intestinal colonization and organ invasion: S. Enteritidis colonises the chicken intestine and is much less invasive to the organs (liver and spleen) of chicks, consequently causing little or no clinical disease in these animals.…”

Section: Patho-biology Of Different Salmonella Serovars In Laying Hensmentioning

confidence: 99%

“…It is assumed that the SEF14 fimbriae, produced by both S. Entertidis and S. Gallinarum, may be involved in reproductive tissue colonisation (Turcotte and Woodward, 1993), and the enhanced survival at 42ºC may also allow S. Enteritidis to contaminate egg contents more successfully than other serovars such as S. Typhimurium, S. Hadar, S. Heidelberg, and S. Virchow (Gantois et al, 2008). The study on roles of the two important type-three secretion systems (T3SS-1 and T3SS-2, encoded on SPI-1 and SPI-2) during S. Enteritidis infection of primary chicken oviduct epithelial cells (COEC) and macrophages has shown that both T3SS are required by S. Enteritidis to invade oviduct cells and to survive in these and in peripheral blood derived macrophages (Li et al, 2009).…”

Section: B Salmonella Contamination Of the Egg Contentsmentioning

confidence: 99%

Scientific Opinion on a quantitative estimation of the public health impact of setting a new target for the reduction of Salmonella in laying hens

Andréoletti¹,

Budka²,

Bunčić³

et al. 2010

EFS2

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European Food Safety Authority (EFSA), Parma, Italy ABSTRACTPublic health risks of Salmonella infection in laying hens (Gallus gallus) can be associated with exposure through four different pathways: internally contaminated table eggs, externally contaminated table eggs, egg products and meat from spent hens. In relation to eggs, Salmonella Enteritidis is by far the serovar most frequently associated with human illness, and exposure through eggs that are internally contaminated with this serovar has a higher public health significance than exposure to externally contaminated eggs. A mathematical model, using reported field data from two EU Member States (MSs), suggests a linear relationship between the investigated scenarios of flock prevalence for Salmonella Enteritidis and the number of contaminated eggs that would be laid. However, the absolute public health impact of the assessed flock prevalence scenarios is highly uncertain due to lack of data on the number of contaminated eggs produced by infected flocks and on the true number of egg-related human salmonellosis cases. It is suggested that public health benefits, similar to those obtained reaching lower Salmonella flock prevalences, may be achieved by implementing controls based on more sensitive sampling protocols. Diversion of eggs from flocks that are tested positive in the EU Salmonella control programme to the production of egg products subjected to heat treatment may lead to increased health risks as heat treatment of egg products should not be considered an absolute barrier to Salmonella contamination. Fresh meat from spent laying hens might carry a higher prevalence of Salmonella than meat from broiler flocks, in particular if sourced from Salmonella-positive flocks. The quantification of under-ascertainment and underreporting of human salmonellosis cases, improving knowledge on within-flock dynamics of Salmonella and harvesting data on production of Salmonella contaminated eggs under field conditions would contribute to improving the accuracy of future quantitative estimates. KEY WORDSSalmonella, microbiological target, laying hen, eggs, quantitative microbiological risk assessment. SUMMARYFollowing a request from the European Commission, the Panel on Biological Hazards (BIOHAZ) was asked to deliver a Scientific Opinion on a quantitative estimate of the public health impact of setting a new target for the reduction of Salmonella in laying hens. In particular, EFSA was asked to assess the relative public health impact if a new target for reduction of Salmonella is set for laying hen flocks being 1% or less remaining positive for all Salmonella serovars with public health significance, compared to (1) a theoretical prevalence of 2% of flocks remaining positive for Salmonella Enteritidis or Salmonella Typhimurium, and (2) the real prevalence in 2008 to be reported by the Member States (MSs).For this task, the BIOHAZ Panel took into account data from the first year of the harmonised EUwide monitoring and control programme of Salmonella in laying hens...

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Cloning, DNA nucleotide sequence and distribution of the gene encoding the SEF14 fimbrial antigen of Salmonella entevitidis

Cited by 61 publications

References 15 publications

Genomic analysis and growth-phase-dependent regulation of the SEF14 fimbriae of Salmonella enterica serovar Enteritidis The GenBank accession number for the sequence reported in this paper is AF239978.

Genomic analysis and growth-phase-dependent regulation of the SEF14 fimbriae of Salmonella enterica serovar Enteritidis The GenBank accession number for the sequence reported in this paper is AF239978.

Distribution, gene sequence and expressionin vivoof the plasmid encoded fimbrial antigen ofSalmonellaserotype Enteritidis

Scientific Opinion on a quantitative estimation of the public health impact of setting a new target for the reduction of Salmonella in laying hens

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