Cloning, expression and subcellular distribution of a Rana grylio virus late gene encoding ERV1 homologue

Fei, Ke; Zhao, Zhe; Zhang, Qi-Ya

doi:10.1007/s11033-008-9365-6

Cited by 16 publications

(14 citation statements)

References 37 publications

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“…Transfected cells were incubated 24 h at 25°C and then fixed and stained with Hoechst 33342 as described previously [51]. All samples were examined under a Leica DM IRB fluorescence microscope.…”

Section: Methodsmentioning

confidence: 99%

Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site

Fei

Pei

et al. 2011

BMC Genomics

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BackgroundA virus was isolated from diseased turbot Scophthalmus maximus in China. Biophysical and biochemical assays, electron microscopy, and genome electrophoresis revealed that the virus belonged to the genus Aquareovirus, and was named Scophthalmus maximus reovirus (SMReV). To the best of our knowledge, no complete sequence of an aquareovirus from marine fish has been determined. Therefore, the complete characterization and analysis of the genome of this novel aquareovirus will facilitate further understanding of the taxonomic distribution of aquareovirus species and the molecular mechanism of its pathogenesis.ResultsThe full-length genome sequences of SMReV were determined. It comprises eleven dsRNA segments covering 24,042 base pairs and has the largest S4 genome segment in the sequenced aquareoviruses. Sequence analysis showed that all of the segments contained six conserved nucleotides at the 5' end and five conserved nucleotides at the 3' end (5'-GUUUUA ---- UCAUC-3'). The encoded amino acid sequences share the highest sequence identities with the respective proteins of aquareoviruses in species group Aquareovirus A. Phylogenetic analysis based on the major outer capsid protein VP7 and RNA-dependent RNA polymerase were performed. Members in Aquareovirus were clustered in two groups, one from fresh water fish and the other from marine fish. Furthermore, a fusion associated small transmembrane (FAST) protein NS22, which is translated from a non-AUG start site, was identified in the S7 segment.ConclusionsThis study has provided the complete genome sequence of a novel isolated aquareovirus from marine fish. Amino acids comparison and phylogenetic analysis suggested that SMReV was a new aquareovirus in the species group Aquareovirus A. Phylogenetic analysis among aquareoviruses revealed that VP7 could be used as a reference to divide the aquareovirus from hosts in fresh water or marine. In addition, a FAST protein with a non-AUG start site was identified, which partially contributed to the cytopathic effect caused by the virus infection. These results provide new insights into the virus-host and virus-environment interactions.

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Section: Methodsmentioning

confidence: 99%

Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site

Fei

Pei

et al. 2011

BMC Genomics

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“…RGV has been recognized as a member of the family Iridoviridae and is closely related to FV3, based on previous studies on morphogenesis, cellular interaction, antigenicity, restriction fragment length polymorphism (RFLP) and major capsid protein (MCP) sequence similarity [15][16][17][18]. To date, some genes of RGV have been identified and characterized, such as 3b-hydroxysteroid dehydrogenase (3b-HSD), deoxyuridine triphosphatase (dUTPase), an envelope protein gene (53R), thymidine kinase (TK) and a gene belonging to the ''essential for respiration and viability'' family (ERV1) [19][20][21][22][23][24]. We found that RGV could induce apoptosis mediated by mitochondria [25].…”

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confidence: 99%

Sequencing and analysis of the complete genome of Rana grylio virus (RGV)

Zhu

et al. 2012

Arch Virol

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Infection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses.

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“…A 9GL deletion mutant displayed a 2-log drop in viral yield, and, of the virions that formed, 90-99 % contained acentric nucleoid structures. Like ASFV, the RGV ERV1 homolog (88R) is a late protein (Ke et al 2009 ). It contains the highly conserved ERV1 motif Cys-X-X-Cys and was detected in both the nucleus and cytoplasm.…”

Section: Erv1mentioning

confidence: 99%