Cloning, Expression, Characterization, and Antioxidant Protection of Glutaredoxin3 From Psychrophilic Bacterium Psychrobacter sp. ANT206

Wang, Yatong; Hou, Yanhua; Wang, Quanfu

doi:10.3389/fmicb.2021.633362

Cited by 3 publications

(3 citation statements)

References 42 publications

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“…GRXs can act as thioltransferase using GSH as the electron donor and disulfide substrate (RSSR) as electron acceptor (Figure 9). HED has been used as a classical RSSR substrate, and l ‐cystine has also been used as a substrate in lots of studies (Holmgren and Aslund, 1995; Manta et al., 2019; Wang et al., 2021; Zaffagnini et al., 2008). Both HED and l ‐cystine have intramolecular disulfide bonds and GRX can catalyze thioldisulfide exchanges between GSH and disulfide substrates (HED and l ‐cystine).…”

Section: Discussionmentioning

confidence: 99%

Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches

Jiang

et al. 2022

The Plant Journal

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Enzymes are essential components of all biological systems. The key characteristics of proteins functioning as enzymes are their substrate specificities and catalytic efficiencies. In plants, most genes encoding enzymes are members of large gene families. Within such families, the contributions of active site motifs to the functional divergence of duplicate genes have not been well elucidated. In this study, we identified 41 glutaredoxin (GRX) genes in the Populus trichocarpa genome. GRXs are ubiquitous enzymes in plants that play important roles in developmental and stress tolerance processes. In poplar, GRX genes were divided into four classes based on clear differences in gene structure and expression pattern, subcellular localization, enzymatic activity, and substrate specificity of the encoded proteins. Using site-directed mutagenesis, this study revealed that the divergence of the active site motif among different classes of GRX proteins resulted in substrate switches and thus provided new insights into the molecular evolution of these important plant enzymes.

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Section: Discussionmentioning

confidence: 99%

Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches

Jiang

et al. 2022

The Plant Journal

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“…This genus is known to be widely distributed in different habitats, including fish, food, clinical specimens, Antarctic soils, and sea water [86]. Psychrobacter is a good producer of antioxidants involved in oxidative stress defence in Antarctic and Arctic environments [87][88][89].…”

Section: Proteobacteriamentioning

confidence: 99%

Biodiversity of UV-Resistant Bacteria in Antarctic Aquatic Environments

Coppola

Lauritano

Zazo

et al. 2023

JMSE

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Antarctica is an untapped reservoir of bacterial communities, which are able to adapt to a huge variety of strategies to cope with extreme conditions and, therefore, are capable of producing potentially valuable compounds for biotechnological applications. In this study, 31 UV-resistant bacteria collected from different Antarctic aquatic environments (surface sea waters/ice and shallow lake sediments) were isolated by UV-C assay and subsequently identified. A phylogenetic analysis based on 16S rRNA gene sequence similarities showed that the isolates were affiliated with Proteobacteria, Actinobacteria and Firmicutes phyla, and they were clustered into 15 bacterial genera, 5 of which were Gram negative (Brevundimonas, Qipengyuania, Sphingorhabdus, Sphingobium, and Psychrobacter) and 10 of which were Gram positive (Staphylococcus, Bacillus, Mesobacillus, Kocuria, Gordonia, Rhodococcus, Micrococcus, Arthrobacter, Agrococcus, and Salinibacterium). Strains belonging to Proteobacteria and Actinobacteria phyla were the most abundant species in all environments. The genus Psychrobacter was dominant in all collection sites, whereas bacteria belonging to Actinobacteria appeared to be the most diverse and rich in terms of species among the investigated sites. Many of these isolates (20 of 31 isolates) were pigmented. Bacterial pigments, which are generally carotenoid-type compounds, are often involved in the protection of cells against the negative effects of UV radiation. For this reason, these pigments may help bacteria to successfully tolerate Antarctic extreme conditions of low temperature and harmful levels of UV radiation.

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“…Recently, Grx from Antarctic Psychrobacter sp. ANT206 (PsGrx) was reported [23]. The optimal temperature for PsGrx activity is 25 • C, and the enzyme retains almost 40% residual activity at even 0 • C, demonstrating that it is a cold-adapted enzyme.…”

Section: Introductionmentioning

confidence: 99%

Glutaredoxin Interacts with GR and AhpC to Enhance Low-Temperature Tolerance of Antarctic Psychrophile Psychrobacter sp. ANT206

Wang

Hou

et al. 2022

IJMS

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Glutaredoxin (Grx) is an important oxidoreductase to maintain the redox homoeostasis of cells. In our previous study, cold-adapted Grx from Psychrobacter sp. ANT206 (PsGrx) has been characterized. Here, we constructed an in-frame deletion mutant of psgrx (Δpsgrx). Mutant Δpsgrx was more sensitive to low temperature, demonstrating that psgrx was conducive to the growth of ANT206. Mutant Δpsgrx also had more malondialdehyde (MDA) and protein carbonylation content, suggesting that PsGrx could play a part in the regulation of tolerance against low temperature. A yeast two-hybrid system was adopted to screen interacting proteins of 26 components. Furthermore, two target proteins, glutathione reductase (GR) and alkyl hydroperoxide reductase subunit C (AhpC), were regulated by PsGrx under low temperature, and the interactions were confirmed via bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation (Co-IP). Moreover, PsGrx could enhance GR activity. trxR expression in Δpsgrx, Δahpc, and ANT206 were illustrated 3.7, 2.4, and 10-fold more than mutant Δpsgrx Δahpc, indicating that PsGrx might increase the expression of trxR by interacting with AhpC. In conclusion, PsGrx may participate in glutathione metabolism and ROS-scavenging by regulating GR and AhpC to protect the growth of ANT206. These findings preliminarily suggest the role of PsGrx in the regulation of oxidative stress, which could improve the low-temperature tolerance of ANT206.

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Cloning, Expression, Characterization, and Antioxidant Protection of Glutaredoxin3 From Psychrophilic Bacterium Psychrobacter sp. ANT206

Cited by 3 publications

References 42 publications

Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches

Divergence of active site motifs among different classes of Populus glutaredoxins results in substrate switches

Biodiversity of UV-Resistant Bacteria in Antarctic Aquatic Environments

Glutaredoxin Interacts with GR and AhpC to Enhance Low-Temperature Tolerance of Antarctic Psychrophile Psychrobacter sp. ANT206

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