Cloning, Expression, Monoclonal Antibody Preparation of Human Gene NBEAL1 and Its Application in Targeted Imaging of Mouse Glioma

Yang, Hao; Sheng, Ping; Chen, Juxiang; Song, Hua; Ding, Xuehua; Liu, Bin; Lu, Yao; Hu, Guo-han; Cui, Daxiang

doi:10.5101/nbe.v1i1.p50-56

Cited by 7 publications

(5 citation statements)

References 24 publications

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“…Nanoparticles (NPs) and nanosized objects are being incorporated rapidly into clinical medicine and particularly into the field of medical oncology [ 27 ]. As the energy donor, quantum dots have the possibility for energy transfer between quantum dot particles and cell molecules such as active oxygen and give them a potential to induce generation of reactive oxygen species and/or free radicals and to provoke apoptosis of the cells [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

The Photodynamic Effect of Different Size ZnO Nanoparticles on Cancer Cell Proliferation In Vitro

et al. 2010

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Nanomaterials have widely been used in the field of biological and biomedicine, such as tissue imaging, diagnosis and cancer therapy. In this study, we explored the cytotoxicity and photodynamic effect of different-sized ZnO nanoparticles to target cells. Our observations demonstrated that ZnO nanoparticles exerted dose-dependent and time-dependent cytotoxicity for cancer cells like hepatocellular carcinoma SMMC-7721 cells in vitro. Meanwhile, it was observed that UV irradiation could enhance the suppression ability of ZnO nanoparticles on cancer cells proliferation, and these effects were in the size-dependent manner. Furthermore, when ZnO nanoparticles combined with daunorubicin, the related cytotoxicity of anticancer agents on cancer cells was evidently enhanced, suggesting that ZnO nanoparticles could play an important role in drug delivery. This may offer the possibility of the great potential and promising applications of the ZnO nanoparticles in clinical and biomedical areas like photodynamic cancer therapy and others.

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Section: Introductionmentioning

confidence: 99%

The Photodynamic Effect of Different Size ZnO Nanoparticles on Cancer Cell Proliferation In Vitro

et al. 2010

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“…For example, a broad range of nanoscale inorganic particles including magnetic nanoparticles (MNPs) and quantum dots (QDs) have been systematically investigated for their unique physical, chemical properties, and their potential application in bio-detection, molecular imaging, and photothermal therapy of tumors [ 8 - 16 ]. Especially, MNPs have been used for magnetic resonance imaging (MRI), gene delivery, cell separation and cancer hyperthermia [ 17 - 19 ], and magnetic targeting, which provides an alternative method for targeted drug delivery systems to the desired location [ 20 - 22 ]. Up to date, QDs have been subjected to intensive investigations due to their unique properties and potential application prospect [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles

et al. 2011

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Fluorescent magnetic nanoparticles exhibit great application prospects in biomedical engineering. Herein, we reported the effects of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles (FMNPs) on human embryonic kidney 293 (HEK293) cells and mice with the aim of investigating their biocompatibility. FMNPs with 150 nm in diameter were prepared, and characterized by high-resolution transmission electron microscopy and photoluminescence (PL) spectra and magnetometer. HEK293 cells were cultured with different doses of FMNPs (20, 50, and 100μ g/ml) for 1-4 days. Cell viability and adhesion ability were analyzed by CCK8 method and Western blotting. 30 mice were randomly divided into three groups, and were, respectively, injected via tail vein with 20, 60, and 100 μg FMNPs, and then were, respectively, raised for 1, 7, and 30 days, then their lifespan, important organs, and blood biochemical parameters were analyzed. Results show that the prepared water-soluble FMNPs had high fluorescent and magnetic properties, less than 50 μg/ml of FMNPs exhibited good biocompatibility to HEK293 cells, the cell viability, and adhesion ability were similar to the control HEK293 cells. FMNPs primarily accumulated in those organs such as lung, liver, and spleen. Lung exposed to FMNPs displayed a dose-dependent inflammatory response, blood biochemical parameters such as white blood cell count (WBC), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), displayed significant increase when the FMNPs were injected into mice at dose of 100μg. In conclusion, FMNPs exhibit good biocompatibility to cells under the dose of less than 50 μg/ml, and to mice under the dose of less than 2mg/kg body weight. The FMNPs' biocompatibility must be considered when FMNPs are used for in vivo diagnosis and therapy.

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“…Particle science has been at the forefront of nanotechnology and fine-particle such as the one which has special structure and good biocompability since the early 1990s. Nowadays nanoparticles are used in broad fields such as targeted drug delivery, biomedical applications, tissue engineering, food and cosmetics [21][22][23][24].…”

Section: Resultsmentioning

confidence: 99%

Erythropoietin Nanopariticles: Therapy for Cerebral Ischemic Injury and Metabolize in Kidney

Wang¹,

Hu²,

Zhang³

et al. 2010

Nano BioMed ENG

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Ischemic metabolism in the enterogram is a crucial issue of clinical concern for deep understanding of drug delivery. In this study we use nanochemistry in micelle to produce the nanocarrier of erythropoietin (EPO) and traced the metabolizable process of the EPO carrier in vivo. Nascence Sprague-Dawley rats of 5 days are treated with EPO-loading nanocarriers. Curative effects of the EPO nanocarriers for periventricular leukomalacia (PVL) models are validated by the analysis of pathology and praxiology of the mice. We demonstrate in vivo that EPO nanocarriers can ameliorate drug-induced liquefaction caused by hypoxia. By tracking the metabolism of EPO in liver and kidney, we suggest that nanocarriers effectively prolong the metabolic half-life and clearance time of EPO. HPLC results show that exciting amino acid toxicity was inhibited, since mobilization of late oligodendrocyte can be protected by treatment of EPO vehicles from hypoxia.

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Cloning, Expression, Monoclonal Antibody Preparation of Human Gene NBEAL1 and Its Application in Targeted Imaging of Mouse Glioma

Cited by 7 publications

References 24 publications

The Photodynamic Effect of Different Size ZnO Nanoparticles on Cancer Cell Proliferation In Vitro

The Photodynamic Effect of Different Size ZnO Nanoparticles on Cancer Cell Proliferation In Vitro

Biocompatibility of hydrophilic silica-coated CdTe quantum dots and magnetic nanoparticles

Erythropoietin Nanopariticles: Therapy for Cerebral Ischemic Injury and Metabolize in Kidney

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