Cloning, molecular characterization and functional analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) gene for diterpenoid tanshinone biosynthesis in Salvia miltiorrhiza Bge. f. alba

Hao, Gangping; Ren-jiu, Shi; Tao, Ran; Fang, Qian; Jiang, Xingyu; Ji, Haiwei; Feng, Lu; Huang, Lu-Qi

doi:10.1016/j.plaphy.2013.05.010

Cited by 34 publications

(21 citation statements)

References 32 publications

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“…For thousands of years, S. miltiorrhiza has been widely used for the treatment of cardiovascular disease, amenorrhea and dysmenorrhea 6 . Tanshinones, a major group of pharmacologically active constituents of this plant, mainly accumulate in the root periderm, and their biosynthesis is regulated by MeJA 7 8 9 . Although numerous chemical and pharmaceutical studies of S. miltiorrhiza have been conducted, investigations of transcription factors are limited, especially with regard to the regulation of transcription factors involved in tanshinone biosynthesis.…”

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confidence: 99%

Genome-wide characterisation and analysis of bHLH transcription factors related to tanshinone biosynthesis in Salvia miltiorrhiza

Luo

Zhu

et al. 2015

Sci Rep

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Salvia miltiorrhiza Bunge (Labiatae) is an emerging model plant for traditional medicine, and tanshinones are among the pharmacologically active constituents of this plant. Although extensive chemical and pharmaceutical studies of these compounds have been performed, studies on the basic helix-loop-helix (bHLH) transcription factors that regulate tanshinone biosynthesis are limited. In our study, 127 bHLH transcription factor genes were identified in the genome of S. miltiorrhiza, and phylogenetic analysis indicated that these SmbHLHs could be classified into 25 subfamilies. A total of 19 sequencing libraries were constructed for expression pattern analyses using RNA-Seq. Based on gene-specific expression patterns and up-regulated expression patterns in response to MeJA treatment, 7 bHLH genes were revealed as potentially involved in the regulation of tanshinone biosynthesis. Among them, the gene expression of SmbHLH37, SmbHLH74 and SmbHLH92 perfectly matches the accumulation pattern of tanshinone biosynthesis in S. miltiorrhiza. Our results provide a foundation for understanding the molecular basis and regulatory mechanisms of bHLH transcription factors in S. miltiorrhiza.

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confidence: 99%

Genome-wide characterisation and analysis of bHLH transcription factors related to tanshinone biosynthesis in Salvia miltiorrhiza

Luo

Zhu

et al. 2015

Sci Rep

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“…Although HDR gene has been cloned from many plants, such as A. thaliana 25 , Ginkgo biloba 26 and Salvia miltiorrhizae Bge. G. alba 27 , there is no report on cloning and characterization of the T. wilfordii gene encoding HDR. In this study, we examined the biosynthesis pathway of terpenoid in T. wilfordii by cloning the HDR gene for the first time.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and functional identification of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Tripterygium wilfordii

Cheng

Tong

Wang

et al. 2017

Acta Pharmaceutica Sinica B

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The 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR) is the last step key enzyme of the methylerythritol phosphate (MEP) pathway, synthesizing isopentenyl diphosphate and its allyl isomer dimethylallyl diphosphate, which is important for regulation of isoprenoid biosynthesis. Here the full-length cDNA of HDR, designated TwHDR (GenBank Accession No. KJ933412.1), was isolated from Tripterygium wilfordii for the first time. TwHDR has an open reading frame (ORF) of 1386 bp encoding 461 amino acids. TwHDR exhibits high homology with HDRs of other plants, with an N-terminal conserved domain and three conserved cysteine residues. TwHDR cDNA was cloned into an expression vector and transformed into an Escherichia coli hdr mutant. Since loss-of-function E.coli hdr mutant is lethal, the result showed that transformation of TwHDR cDNA rescued the E.coli hdr mutant. This complementation assay suggests that the TwHDR cDNA encodes a functional HDR enzyme. The expression of TwHDR was induced by methyl-jasmonate (MJ) in T. wilfordii suspension cells. The expression of TwHDR reached the highest level after 1 h of MJ treatment. These results indicate that we have identified a functional TwHDR enzyme, which may play a pivotal role in the biosynthesis of diterpenoid triptolide in T. wilfordii.

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“…The content of caffeic acid-derived phenolic acids in S. miltiorrhiza Bge. f. alba is approximately twice that in S. miltiorrhiza Bunge (Hao et al 2013). Jiao et al (2007) have reported that S. miltiorrhiza Bge.…”

Section: Introductionmentioning

confidence: 92%

“…In addition to this difference, S. miltiorrhiza Bge. f. alba has unique pharmacological effects and is used for the treatment of thromboangiitis obliterans (Hao et al 2013). The content of caffeic acid-derived phenolic acids in S. miltiorrhiza Bge.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we successfully constructed a full-length cDNA library from white-flowered S. miltiorrhiza Bge. f. alba roots (Hao et al 2009), performed cloning and functional analysis of the SmPDC and SmHDR genes (Hao et al 2013) and successfully induced hairy roots from the leaves of S. miltiorrhiza Bge. f. alba using A. rhizogenes ACCC10060 .…”

Section: Introductionmentioning

confidence: 99%

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Cloning, molecular characterization and functional analysis of a putative R2R3-MYB transcription factor of the phenolic acid biosynthetic pathway in S. miltiorrhiza Bge. f. alba

Hao

Jiang

Feng

et al. 2015

Plant Cell Tiss Organ Cult

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The water-soluble phenolic acids of S. miltiorrhiza Bge. f. alba can protect against herbivores or pathogenic bacteria and have numerous pharmacological activities that are beneficial to human health. The main phenolic acids in S. miltiorrhiza, including rosmarinic acid and salvianolic acid B, are derived from the rosmarinic acid biosynthetic pathway, which has been partially characterized. However, little is known about the endogenous bHLH and MYB transcription factors that function in this pathway. In this paper, we describe the cloning and functional characterization of SmPAP1, a cDNA of the R2R3-MYB transcription factor isolated from S. miltiorrhiza Bge. f. alba. SmPAP1 contains an open reading frame of 645 bp in length and encodes a putative 214-amino acid protein.The deduced amino acid sequence of the N-terminal R2R3 repeat sequence of SmPAP1 shares high identity with other DNA-binding domains of plant MYB-type proteins.

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Cloning, molecular characterization and functional analysis of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (HDR) gene for diterpenoid tanshinone biosynthesis in Salvia miltiorrhiza Bge. f. alba

Cited by 34 publications

References 32 publications

Genome-wide characterisation and analysis of bHLH transcription factors related to tanshinone biosynthesis in Salvia miltiorrhiza

Genome-wide characterisation and analysis of bHLH transcription factors related to tanshinone biosynthesis in Salvia miltiorrhiza

Molecular cloning and functional identification of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Tripterygium wilfordii

Cloning, molecular characterization and functional analysis of a putative R2R3-MYB transcription factor of the phenolic acid biosynthetic pathway in S. miltiorrhiza Bge. f. alba

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