Cloning of <i>MeCWINV3</i> Promoter from Cassava and Transient Expression Analysis in Tobacco

Abstract

In order to study the inducement pattern and regulating mechanism of MeCWINV3 in Cassava. An 1160 bp promoter region upstream of the MeCWINV3 gene (GenBank Accession No. KC905170) was isolated from Cassava (Manihot esculenta) genomic DNA using PCR methods. Sequence analysis found that it contains typical TATA box and CAAT box, and several cis-acting elements that related plant stress responses, such as ABRE, ARFAT, GAREAT, MYB and MYC transcription factors. Furthermore, transient expression in transgenic tobacco was analyzed by inserting upstream of GUS gene in expressing vector. The results showed that GUS was mainly expressed in tobacco veins. This will be the basis for further investigating the function of the MeCWINV3 gene promoter.

Regulation of Maize Kernel Carbohydrate Metabolism by Abscisic Acid Applied at the Grain-Filling Stage at Low Soil Water Potential

“…Susan (1999) found that the miniature seed phenotype of the Incw2 deficient mutant could not be restored even under conditions of exogenous hexose application [53]. Similarly, another specific CWIN in tobacco was inhibited [54] and, even if glucose and fructose were supplied exogenously, pollen development could not be completely restored [55]. These studies suggest that CWIN may regulate the release of hexose through sugar signaling rather than just supplying nutrients.…”