Cloning of peroxisome proliferators activated receptors in the cobia (Rachycentron canadum) and their expression at different life-cycle stages under cage aquaculture

Peroxisome proliferator-activated receptor gamma (PPARγ) in yellow catfish Pelteobagrus fulvidraco: Molecular characterization, mRNA expression and transcriptional regulation by insulin in vivo and in vitro

“…The analysis of tissue distribution for PPARc was useful to better our understanding of its physiological roles. In the present study, PPARc gene was widely expressed in all the tested tissues, in agreement with other studies (Leaver et al, 2005;Tsai et al, 2008). This ubiquitous spatial distribution indicated that most tissues were potential targets of PPARc and PPARc was involved in many metabolic pathways.…”

“…PPARc transcript was weakly expressed in spleen in yellow catfish, in agreement with the reports in other fish (Batista-Pinto et al, 2005;Tsai et al, 2008), but in contrast with high expression in rodents (Lemberger et al, 1996;Corton et al, 2000). These discrepancies may be due to differences in organ constitution and functions among species.…”

“…The mRNA level of PPARc was the highest in adipose tissue in yellow catfish, in agreement with its physiological role in fat accumulation and adipocyte differentiation (Rosen and Spiegelman, 2001). Similarly, PPARc was highly expressed in adipose tissue in other fish (Boukouvala et al, 2004;Tsai et al, 2008;Cho et al, 2009;Zheng et al, 2013). Hepatic PPARc expression was also high in yellow catfish, similar to those in plaice (Leaver et al, 1998) and salmon (Andersen et al, 2000).…”

“…To date, three PPAR isotypes-a, b and c, encoded by separate genes and showing different tissue distribution patterns have been identified (Desvergne and Wahli, 1999;Escher et al, 2001). Among three PPAR isotypes, PPARc is highly expressed in adipose tissue and has important roles in lipid metabolism, cell proliferation and inflammation (Tsai et al, 2008). In mammals, two transcripts of PPARc with different lengths of the N-terminal, c1 and c2, have been found in mice (Tontonoz et al, 1994b;Zieleniak et al, 2008).…”

“…At present, PPARc has been identified and cloned in many fish species. For example, a single PPARc transcript has been identified in Rachycentron canadum (Tsai et al, 2008), Paralichthys olivaceus (Cho et al, 2009), Dicentrarchus labrax (Boukouvala et al, 2004), Salmo trutta (Batista-Pinto et al, 2005), Pleuronectes platessa and Sparus aurata (Leaver et al, 2005), Takifugu rubripes (Kondo et al, 2007), Danio rerio (Ibabe et al, 2005) and Chelon labrosus (Raingeard et al, 2009). However, the exact number of genes and/or the presence of distinct PPARc have not been determined in fish.…”