Cloning of Pigs from Somatic Cells and Its Prospects

Ōnishi, Akira

doi:10.1089/15362300260339539

Cited by 8 publications

(3 citation statements)

References 34 publications

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“…Although efficiency is low, cloned cattle surviving to adulthood have growth and reproductive performance similar to those of cattle produced by artificial insemination or natural breeding [31]. In contrast, although the efficiency of pregnancy is extremely low in somatic cell clones in pigs, cloned piglets have fewer abnormalities in the postnatal period [32]. From these results, we inferred that the cloned sows and their live-born progenies showed no abnormalities and had normal growth performance.…”

Section: Resultsmentioning

confidence: 99%

Growth, Reproductive Performance, Carcass Characteristics and Meat Quality in F1 and F2 Progenies of Somatic Cell-Cloned Pigs

Adachi

Yamaguchi

Watanabe

et al. 2014

Journal of Reproduction and Development

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The objective of this study was to examine the health and meat production of cloned sows and their progenies in order to demonstrate the application of somatic cell cloning to the pig industry. This study compared the growth, reproductive performance, carcass characteristics and meat quality of Landrace cloned sows, F1 progenies and F2 progenies. We measured their body weight, growth rate and feed conversion and performed a pathological analysis of their anatomy to detect abnormalities. Three of the five cloned pigs were used for a growth test. Cloned pigs grew normally and had characteristics similar to those of the control purebred Landrace pigs. Two cloned gilts were bred with a Landrace boar and used for a progeny test. F1 progenies had characteristics similar to those of the controls. Two of the F1 progeny gilts were bred with a Duroc or Large White boar and used for the progeny test. F2 progenies grew normally. There were no biological differences in growth, carcass characteristics and amino acid composition among cloned sows, F1 progenies, F2 progenies and conventional pigs. The cloned sows and F1 progenies showed normal reproductive performance. No specific abnormalities were observed by pathological analysis, with the exception of periarteritis in the F1 progenies. All pigs had a normal karyotype. These results demonstrate that cloned female pigs and their progenies have similar growth, reproductive performance and carcass quality characteristics and that somatic cell cloning could be a useful technique for conserving superior pig breeds in conventional meat production.

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Section: Resultsmentioning

confidence: 99%

Growth, Reproductive Performance, Carcass Characteristics and Meat Quality in F1 and F2 Progenies of Somatic Cell-Cloned Pigs

Adachi

Yamaguchi

Watanabe

et al. 2014

Journal of Reproduction and Development

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“…However, the effects of caffeine during oocyte aging on embryonic development have not yet been studied. It is also considered that exposure of somatic nuclei to recipient cytoplasm with high MPF activity is an important factor in the induction of reprogramming of the injected somatic nucleus (Wakayama et al, 1998;Onishi, 2002). One of the important phenomena that occurs in the injected somatic cell nucleus is 'premature chromosome condensation' (PCC) (Czolowska et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Effects of caffeine treatment on aged porcine oocytes: parthenogenetic activation ability, chromosome condensation and development to the blastocyst stage after somatic cell nuclear transfer

Iwamoto

Ōnishi

Fuchimoto

et al. 2005

Zygote

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The possibility of using aged porcine oocytes treated with caffeine, which inhibits the decrease in M-phase promoting factor activity, for pig cloning was evaluated. Cumulus-oocyte complexes (COCs) were cultured initially for 36 h and subsequently with or without 5 mM caffeine for 24 h (in total for 60 h: 60CA+ or 60CA- group, respectively). As a control group, COCs were cultured for 48 h without caffeine (48CA-). The pronuclear formation rates at 10 h after electrical stimulation in the 60CA+ and 60CA- groups decreased significantly (p < 0.05) compared with the 48CA- group. However, the fragmentation rate was significantly higher (p < 0.05) in the 60CA- group than in the 60CA+ and 48CA- groups. When the stimulated oocytes were cultured for 6 days, the 60CA+ group showed significantly lower blastocyst formation and higher fragmentation or degeneration rates (p < 0.05) than the 48CA- group. However, the number of total cells in blastocysts was not affected by maturation period or caffeine treatment. When somatic cell nuclei were injected into the non-enucleated oocytes and exposed to cytoplasm for a certain duration (1-11 h) before the completion of maturation (48 or 60 h), the rate of nuclear membrane breakdown after exposure to cytoplasm for 1-2 h in the 60CA- oocytes was significantly lower (p < 0.05) than in the other experimental groups. The rate of scattered chromosome formation in the same 60CA- group tended to be lower (p = 0.08) than in the other groups. After the enucleation and transfer of nuclei, blastocyst formation rates in the 60CA+ and 60CA- groups were significantly lower (p < 0.05) than in the 48CA- group. Blastocyst quality did not differ among all the groups. These results suggest that chromosome decondensation of the transplanted somatic nucleus is affected by both the duration of exposure to cytoplasm and the age of the recipient porcine oocytes, and that caffeine treatment promotes nuclear remodelling but does not prevent the decrease in the developmental ability of cloned embryos caused by oocyte aging.

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“…Since then, interest in the use of cloned pigs has grown largely because of their potential utility for medical research [3,4]. Somatic cell cloning is also expected to play a part in integrated strategies for conserving pig genetic resources, as cryopreservation of pig embryos is still in development, whereas that for somatic cells is well-established [5]. For the practical application of somatic cell cloning for conservation of genetic resources, it is essential that somatic cell cloned p i g s a r e n o r m a l a n d t h a t t h e y s h a r e t h e characteristic traits of the progenitor.…”

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confidence: 99%

Reproductive and Growth Performance in Jin Hua Pigs Cloned from Somatic Cell Nuclei and the Meat Quality of Their Offspring

Shibata

Otake

Tsuchiya

et al. 2006

Journal of Reproduction and Development

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Abstract. Somatic cell cloning is expected to be a valuable method for conserving genetic resources in pigs. In this study, we compared the reproductive and growth performance of Jin Hua cloned pigs with that of naturally bred Jin Hua pigs. In addition, we generated offspring from the cloned sows and examined the productivity and quality of meat in the progeny. The birth weights and growth rates of somatic cell-cloned pigs were similar to those of Jin Hua pigs. The cloned pigs reached puberty very early, and this is typical of the Jin Hua breed. Furthermore, reproductive performance, in terms of traits such as gestation period, litter size, and raising rate in the cloned pigs were similar to Jin Hua pigs. Although the offspring of the cloned (OC) pigs had lower birth weights than the Jin Hua breed, the daily weight gain of the OC pigs was significantly higher, especially at the finishing stage. The carcass quality of the OC pigs had similar characteristics to the Jin Hua breed, namely thick back fat and a small loin area. Furthermore, the meat qualities of the OC pigs were similar to those of Jin Hua pigs in terms of intramuscular fat content and tenderness. These results demonstrate that cloned pigs and their offspring were similar to the Jin Hua breed in most of the growth, reproductive, and meat productive performances. This strongly suggests that pigs cloned from somatic cell nuclei have the potential to be a valuable genetic resource for breeding.

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Cloning of Pigs from Somatic Cells and Its Prospects

Abstract: The technology of somatic cell cloning in pigs is valuable for agricultural and therapeutic purposes. This paper will focus on the current methods of cloning pigs, including our successful microinjection#10; of somatic cell nuclei and its application. #10;

Cited by 8 publications

References 34 publications

Growth, Reproductive Performance, Carcass Characteristics and Meat Quality in F1 and F2 Progenies of Somatic Cell-Cloned Pigs

Growth, Reproductive Performance, Carcass Characteristics and Meat Quality in F1 and F2 Progenies of Somatic Cell-Cloned Pigs

Effects of caffeine treatment on aged porcine oocytes: parthenogenetic activation ability, chromosome condensation and development to the blastocyst stage after somatic cell nuclear transfer

Reproductive and Growth Performance in Jin Hua Pigs Cloned from Somatic Cell Nuclei and the Meat Quality of Their Offspring

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