Cloning of Rac and Rho-GDI from tobacco using an heterologous two-hybrid screen*1*The first two authors contributed equally to this work.

Kieffer, F.; Elmayan, Taline; Rubier, Sébastien; Simon-Plas, Françoise; Dagher, Marie-Claire; Blein, Jean‐Pierre

doi:10.1016/s0300-9084(00)01199-8

Cited by 23 publications

(19 citation statements)

References 30 publications

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“…3) and negatively regulates their activation at the membrane [100]. Plant GDIs from Arabidopsis and tobacco were described by their similarity to animal RhoGDIs and were shown to 2454 A. Berken ROP GTPases in signal transduction interact with distinct ROPs in yeast two-hybrid tests and in vitro [101,102]. Their role for the regulation of ROP recruitment to the PM is assumed because overexpression of the Arabidopsis RhoGDI1 removed GFP-tagged ROPs from the PM in tobacco protoplasts and pollen tubes [8,80].…”

Section: Gdis -More Than Just Cytosolic Sequestrationmentioning

confidence: 99%

ROPs in the spotlight of plant signal transduction

Berken

2006

Cell. Mol. Life Sci.

103

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Small guanine nucleotide binding proteins of the Rho family called ROP play a crucial role as regulators of signal transduction in plants. They participate in pathways that influence growth and development, and the adaptation of plants to various environmental situations. As members of the Ras superfamily, ROPs function as molecular switches cycling between a GDP-bound 'off' and a GTP-bound 'on' state in a strictly regulated manner. Latest research provided fascinating new insights into ROP regulation by novel guanine nucleotide exchange factors, unconventional GTPase activating proteins, and guanine nucleotide dissociation inhibitors, which apparently organize localized ROP activation. Important progress has also been made concerning signaling components upstream and downstream of the ROP cycle involving receptor-like serine/threonine kinases and effectors that can manipulate cytoskeletal dynamics, intracellular calcium levels, H2O2 production and further cellular targets. This review outlines the fast developing knowledge on ROP GTPases highlighting their specific features, regulation and roles in a cellular signaling context.

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Section: Gdis -More Than Just Cytosolic Sequestrationmentioning

confidence: 99%

ROPs in the spotlight of plant signal transduction

Berken

2006

Cell. Mol. Life Sci.

103

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“…ROP partner proteins regulate subcellular localization, GTPase activity, and GDP/GTP nucleotide exchange of ROPs, which modulates ROP functions in a spatiotemporal manner. ROP-GDIs are guanine nucleotide dissociation inhibitors supposed to bind C-terminal prenyl residues of GDP-bound RHO proteins and sequester RHO-GDP from the plasma membrane (Kieffer et al, 2000;Mucha et al, 2011). ROP-GEFs are guanine nucleotide exchange factors that facilitate the exchange of GDP for GTP and hence activate ROPs.…”

Section: Introductionmentioning

confidence: 99%

A Barley ROP GTPase ACTIVATING PROTEIN Associates with Microtubules and Regulates Entry of the Barley Powdery Mildew Fungus into Leaf Epidermal Cells

et al. 2011

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Little is known about the function of host factors involved in disease susceptibility. The barley (Hordeum vulgare) ROP (RHO of plants) G-protein RACB is required for full susceptibility of the leaf epidermis to invasion by the biotrophic fungus Blumeria graminis f. sp hordei. Stable transgenic knockdown of RACB reduced the ability of barley to accommodate haustoria of B. graminis in intact epidermal leaf cells and to form hairs on the root epidermis, suggesting that RACB is a common element of root hair outgrowth and ingrowth of haustoria in leaf epidermal cells. We further identified a barley MICROTUBULE-ASSOCIATED ROP-GTPASE ACTIVATING PROTEIN (MAGAP1) interacting with RACB in yeast and in planta. Fluorescent MAGAP1 decorated cortical microtubules and was recruited by activated RACB to the cell periphery. Under fungal attack, MAGAP1-labeled microtubules built a polarized network at sites of successful defense. By contrast, microtubules loosened where the fungus succeeded in penetration. Genetic evidence suggests a function of MAGAP1 in limiting susceptibility to penetration by B. graminis. Additionally, MAGAP1 influenced the polar organization of cortical microtubules. These results add to our understanding of how intact plant cells accommodate fungal infection structures and suggest that RACB and MAGAP1 might be antagonistic players in cytoskeleton organization for fungal entry.

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“…In plants, many members of the Rho subfamily have been identified from different species (Valster et al 2000, Borg et al 1997, Kieffer et al 2000. Surprisingly, almost all the members belong to the Rac type GTPases of three types of the subfamily, Rho, Rac and CDC42 (Winge et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Preferential and Asymmetrical Accumulation of a Rac Small GTPase mRNA in Differentiating Xylem Cells of Zinnia elegans

Nakanomyo

Kost

Chua

et al. 2002

Plant and Cell Physiology

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;Rac-type small GTPases are known to function in some cellular processes in plants. To further understand the involvement of Rac type GTPases in plant development, we isolated from cultured Zinnia cells a gene (ZeRAC2) encoding a new Rac-type small GTPase. ZeRAC2 mRNA accumulates preferentially in xylogenic culture and transiently at the time when visible tracheary elements appear. Experiments with ZeRAC2 recombinant proteins demonstrated that ZeRAC2 binds to and hydrolyzes GTP. A GFPZeRAC2 fusion protein was localized to the plasma membrane. Together with the fact that ZeRAC2 possesses a putative geranylgeranylation site at the C-terminus, this suggests that ZeRAC2 acts on the plasma membrane. In situ hybridization indicated that ZeRAC2 mRNA accumulates preferentially in xylem parenchyma and tracheary element precursor cells, and surprisingly the accumulation is restricted to the site facing developing tracheary elements.

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Cloning of Rac and Rho-GDI from tobacco using an heterologous two-hybrid screen1The first two authors contributed equally to this work.

Cited by 23 publications

References 30 publications

ROPs in the spotlight of plant signal transduction

ROPs in the spotlight of plant signal transduction

A Barley ROP GTPase ACTIVATING PROTEIN Associates with Microtubules and Regulates Entry of the Barley Powdery Mildew Fungus into Leaf Epidermal Cells

Preferential and Asymmetrical Accumulation of a Rac Small GTPase mRNA in Differentiating Xylem Cells of Zinnia elegans

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