Cloning of regulatory sequences mediating guard-cell-specific gene expression

Plesch, Gunnar; Kamann, Eike; Mueller‐Roeber, Bernd

doi:10.1016/s0378-1119(00)00150-5

Cited by 45 publications

(35 citation statements)

References 30 publications

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“…This was supported by the ®nding that the ®rst two promoters identi®ed by T-DNA promoter-trap experiments, pT218 and tCUP, were not associated with expressed genes (Fobert et al 1994;Foster et al 1999). Others have since isolated additional cryptic promoters from plants (O È kreÂ sza et al 1998;Plesch et al 2000). The cryptic promoter T218 is expressed in a cell-speci®c manner (Fobert et al 1994).…”

Section: Discussionmentioning

confidence: 99%

“…It has been generally assumed that activation of such promoterless or enhancerless marker genes results from insertion within or immediately adjacent to expressed genes (Koncz et al 1989;Topping et al 1994;Wei et al 1997). However, further investigations are showing that integration may also activate silent regulatory sequences that are not associated with genes (Lindsey et al 1993;Fobert et al 1994;O È kreÂ sza et al 1998;Foster et al 1999;Plesch et al 2000). Inactive regulatory sequences with the ability to become functional when positioned adjacent to genes have been found in many dierent organisms and are referred to as`c ryptic'' elements (Al-Shawi et al 1991;Fourel et al 1992;Irniger et al 1992;Fobert et al 1994;Troyanovsky and Leube 1994;Tee et al 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Inactive regulatory sequences with the ability to become functional when positioned adjacent to genes have been found in many dierent organisms and are referred to as`c ryptic'' elements (Al-Shawi et al 1991;Fourel et al 1992;Irniger et al 1992;Fobert et al 1994;Troyanovsky and Leube 1994;Tee et al 1995). The isolation and characterization of several cryptic promoters from plants (Fobert et al 1994;O È kreÂ sza et al 1998;Foster et al 1999;Plesch et al 2000) suggests that cryptic gene regulatory elements might be abundant in the plant genome. It is known that the frequent rearrangements, insertions and deletions that occur in intergenic regions can have a profound eect on gene expression patterns in plants by altering gene promoters (Chen et al 1997), and it has been suggested that such events may be pivotal to the evolution of higher organisms (Doebley and Lukens 1998).…”

Section: Introductionmentioning

confidence: 99%

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Enhancers and core promoter elements are essential for the activity of a cryptic gene activation sequence from tobacco, tCUP

Malik

Tian

et al. 2001

Mol Gen Genomics

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Cryptic gene regulatory elements are sequences that are inactive at their native locations in the genome but have the ability to become functional when positioned adjacent to genes. We have recently isolated such a cryptic sequence from tobacco, tCUP, that can act as a promoter. A 135-bp fragment spanning extending from position -197 to -62, relative to the transcription start site, was found to promote GUS expression in all of the major organs of transgenic Arabidopsis plants. Furthermore, this 135-bp fragment complemented the -46 minimal promoter of CaMV 35S and conferred constitutive expression on transgenic Arabidopsis plants. An electrophoretic mobility-shift assay showed that nuclear proteins prepared from tobacco leaves interact with the 135-bp fragment. tCUP has a core promoter that lacks the TATA consensus sequence but addition of a TATA-box sequence increased the core promoter activity by three-fold. The sequence surrounding the transcription start site of tCUP has sequence similarity with the initiator element (Inr), and deletion of this sequence significantly reduced promoter activity, suggesting that an essential Inr element may exist in the tCUP core promoter. Fusion of the GCC-box enhancer element from pathogenesis-related genes to the core promoter elevated tCUP core promoter activity. Our study indicates that cryptic promoters are similar in composition and organization to promoters associated with expressed genes and that their promoter elements can be combined to create composite promoters that are fully functional. This data provides direct evidence that the expression pattern of plant genes can be influenced by cryptic gene regulatory elements when they are brought into juxtaposition with genes through DNA rearrangements.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhancers and core promoter elements are essential for the activity of a cryptic gene activation sequence from tobacco, tCUP

Malik

Tian

et al. 2001

Mol Gen Genomics

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“…There is a possibility that the GUS gene was tagging an antisense transcript found in many annotated genes (Yamada et al 2003). In the line that contained the reporter gene inserted in the intergenic region, the GUS gene may be tagging an unannotated gene or may be controlled by a 'criptic' promoter (Plesch et al 2000). Another possible explanation is that the GUS gene may be inserted in a genome region for non-coding RNA such as a micro RNA precursor.…”

Section: Analysis Of Gus Expression Patterns In Gene Trap Linesmentioning

confidence: 99%

Expression analysis of gene trap lines and mapping of donor loci for Dissociation transposition in Arabidopsis

Kato

Tabata

Sato

2007

Plant Biotechnology

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“…These elements are silent at their native locations within the plant genome but can be activated by rearrangements that position them adjacent to genes in a configuration that promotes gene expression (Fobert et al 1994;Cocherel et al 1996;Okresz et al 1998;Foster et al 1999;Plesch et al 2000;Salgueiro et al 2000). They are believed to be abundant in the plant genome and can generate diverse expression profiles (Fobert et al 1994).…”

Section: Introductionmentioning

confidence: 99%

A constitutive gene expression system derived from the tCUP cryptic promoter elements

Malik

et al. 2002

Theor Appl Genet

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A limited number of constitutive promoters have been used to direct transgene expression in plants and they are often derived from non-plant sources. Here, we describe novel gene-regulatory elements which are associated with a cryptic constitutive promoter from tobacco, tCUP, and modifications that were made to create a strong gene-expression system that is effective across all living cell types from a wide range of plant species, including several important crops ( Arabidopsis, canola, flax, alfalfa, tobacco). The tCUP 5' untranslated region was mutated to eliminate translational interference by upstream ATGs, and the influence of the Kozak consensus sequence on the levels of a beta-glucuronidase (GUS) reporter gene activity was demonstrated. These modifications resulted in expression that was greatly enhanced in all organs. A TATA consensus sequence was added to the core promoter to complement an existing Initiator (Inr) sequence. Although this addition was known to elevate core promoter activity by 3-fold the additive effect on the overall gene-expression system was marginal in all of the transgenic plants tested. Two transcriptional enhancers were identified and the region containing them were oligomerized, yielding a significant increase in marker gene-expression in some but not all plant species. In general, the enhanced tCUP gene-expression system generated levels of GUS activity which exceeded that of the 35S promoter in most plant species and the elevation in activity occurred uniformly among the various plant organs. The potential benefit of cryptic elements for the construction of gene-expression systems for crop species is discussed

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Cloning of regulatory sequences mediating guard-cell-specific gene expression

Cited by 45 publications

References 30 publications

Enhancers and core promoter elements are essential for the activity of a cryptic gene activation sequence from tobacco, tCUP

Enhancers and core promoter elements are essential for the activity of a cryptic gene activation sequence from tobacco, tCUP

Expression analysis of gene trap lines and mapping of donor loci for Dissociation transposition in Arabidopsis

A constitutive gene expression system derived from the tCUP cryptic promoter elements

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