Cloning of repeated DNA sequences from<i>Streptomyces cattleya</i>

Usdin, K.; Kirby, Ralph

doi:10.1111/j.1574-6968.1988.tb02938.x

Cited by 2 publications

(3 citation statements)

References 12 publications

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“…Relaxed recombination in these hypothetical mutants could occur more favourably at the frequently repeated sequences detected in the Streptomyces genome (Usdin and Kirby, 1988). Moreover, the finding of imperfect palindromic sequences at the extremities of AUDs and at the ends of a deletion in S. glaucescens (Hausler ef ai.…”

Section: Genome Plasticitymentioning

confidence: 99%

“…For example, the stability of the replisome-DNA complex might be affected under growth conditions that modify the replication rhythm (particularly treatment with intercalating dyes, spore germination or the resumption of growth, after cold storage), resulting in errors in DNA replication. Such aberrant behaviour could occur at palindromic or short repeated sequences, which are frequent in the G+C-rich (72%) genome of Streptomyces (Usdin and Kirby, 1988). Alternatively indirect effects on DNA replication could trigger DNA damage and consequently an SOS-like response although there is, as yet, no evidence for such a response in Streptomyces.…”

mentioning

confidence: 99%

“…Alternatively indirect effects on DNA replication could trigger DNA damage and consequently an SOS-like response although there is, as yet, no evidence for such a response in Streptomyces. The resulting increase in recombination could cause deletions to occur between repeated sequences (Usdin and Kirby, 1988), Whatever the mutational mechanism, there are many reports of intercalating dyes incjucing deletions either in chromosomal (Hintermann etai, 1984;Schrempf, 1985) or plasmid DNA (Schrempf, 1985;Crameri etai. 1986).…”

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Genetic instability and hypervariability in Streptomyces ambofaciens: towards an understanding of a mechanism of genome plasticity

Leblond

Demuyter

Simonet

et al. 1990

Molecular Microbiology

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Many Streptomyces species exhibit a very high degree of genetic instability which is usually manifested as genomic rearrangements such as large deletions. In Streptomyces ambofaciens DSM40697, two levels of genetic instability were previously described: (i) a basic genetic instability similar to that reported for other strains, and (ii) hypervariability, a phenomenon that we believe to be a new aspect of instability closely associated with DNA amplification. A large DNA region undergoes deletions, amplifications and large genomic changes strictly associated with both aspects of genetic instability. The genetic and molecular analyses of the different aspects of genetic instability allow us to propose that they result from a cascade of molecular events and to investigate the relationships between genetic instability phenomena and genome fluidity.

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Section: Genome Plasticitymentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Genetic instability and hypervariability in Streptomyces ambofaciens: towards an understanding of a mechanism of genome plasticity

Leblond

Demuyter

Simonet

et al. 1990

Molecular Microbiology

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Physical map of the Streptomyces lividans 66 genome and comparison with that of the related strain Streptomyces coelicolor A3(2)

1993

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A physical map of the chromosome of Streptomyces lividans 66 ZX7 was constructed by ordering the macrorestriction fragments generated from the genomic DNA with the restriction enzymes AseI and DraI. AseI and Dral linking cosmids (i.e., recombinant cosmids including eitherAseI orDral sites) were isolated from a gene bank and used as hybridization probes against Southern transfers of pulsed-field gel electrophoresis (PFGE) restriction patterns. The DraI sites were precisely mapped by PFGE analyses of AseI-DraI double digests and hybridization with the AseI junctions. The 16AseI and 7 Dram fragments were aligned as a single chromosome of about 8,000 kb. The data supported the interpretation that the chromosome is a linear structure. The related strain Streptomyces coelicolor A3(2) M145, recently mapped by H. Kieser, T. Kieser, and D. A. Hopwood (J. Bacteriol. 174:5496-5507, 1992), was compared with S. lividans at the level of the genomic structure by hybridizing the linking cosmids to Southern transfers of PFGE patterns. In spite of little apparent similarity in their restriction patterns, the comparison of the physical maps revealed a common structure with an identical ordering of the cosmid sequences. This conservation of the map order was further confirmed by assigning genetic markers (i.e., cloned genes and DNA elements relevant to the unstable region) to the AseI fragments.The genus Streptomyces comprises gram-positive filamentous soil bacteria with a complex life cycle: spores germinate to form a branched, vegetative mycelium which produces an aerial mycelium and, subsequently, spores. The chromosome is present in multiple copies in the vegetative and aerial mycelia but is present as only a single copy in the spores. In addition to this morphological differentiation, the remarkable features of the Streptomyces genus include the extensive secondary metabolism directing the production of various molecules of industrial importance such as hydrolytic enzymes, enzyme inhibitors, and over 60% of naturally occurring antibiotics (3) and the high G+C content of the genomic DNA (70 to 74%) (8). The genome is also characterized by the presence of giant linear plasmids in numerous species (30), multiple insertion sequences (7), and repetitive sequences (43). A remarkable trait of Streptomyces species is the genomic plasticity associated with genetic instability, which is well characterized for Streptomyces lividans (11,14,38) as well as several other Streptomyces species (4,31,32). This plasticity consists of large deletions and extensive amplifications. Thus, mapping the genome would provide a new approach to the investigation of genetic instability at the level of the whole genome.Since the advent of pulsed-field gel electrophoresis (PFGE), the physical mapping of many bacterial genomes has been reported (29 and references therein). As part of our study of genetic instability in S. lividans 66, we wanted to construct the physical map of the chromosome. However, most derivatives of S. lividans 66 are not amenable to P...

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Cloning of repeated DNA sequences fromStreptomyces cattleya

Cited by 2 publications

References 12 publications

Genetic instability and hypervariability in Streptomyces ambofaciens: towards an understanding of a mechanism of genome plasticity

Genetic instability and hypervariability in Streptomyces ambofaciens: towards an understanding of a mechanism of genome plasticity

Physical map of the Streptomyces lividans 66 genome and comparison with that of the related strain Streptomyces coelicolor A3(2)

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