Cloning of the Bovine β-Carotene-15,15’-Oxygenase and Expression in Gonadal Tissues

Morales, A.; Rosas, Antonio; González, Alina Alerm; Antaramián, Anaid; Varela‐Echavarría, Alfredo; Shimada, Armando S.; Mora, Ofelia

doi:10.1024/0300-9831.76.1.9

Cited by 7 publications

(2 citation statements)

References 41 publications

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“…Carotenoids, such as β-carotene, can be converted to vitamin A in cells of the intestinal mucosa, liver, uterus, and ovaries (Chew et al, 1982a;Borel et al, 2005;Morales et al, 2006). Carotenoids also have antioxidant function, protecting cells and cell components against reactive oxygen species (Chew and Park, 2004).…”

Section: Introductionmentioning

confidence: 98%

Supplementation of prepartum dairy cows with β-carotene

Oliveira

Guerreiro

Morais

et al. 2015

Journal of Dairy Science

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The prepartum supplementation of dairy cows with β-carotene was evaluated. Cows were blocked by parity and expected calving date and assigned to a treatment: β-carotene (1.2 g/cow per d) or control (no supplementation). The same total mixed ration batch was offered to all cows, and β-carotene was top dressed to individual cows once per day. The data set contained 283 Holsteins that received a treatment for >14 d (29.1±6.9 d). Frequency distributions were analyzed with the GENMOD procedure of SAS using logistic regression for binomial data. Continuous variables were analyzed with the MIXED procedure of SAS. Within parity, nonparametric estimates of the survivor function for reproductive variables were computed using the product-limit method of the Kaplan-Meier method with the LIFETEST procedure of SAS. Plasma β-carotene concentration before supplementation was similar between supplemented and nonsupplemented cows (2.99µg/mL) and peaked at 3.26±0.175µg/mL on d -15±2.4 precalving for supplemented cows (2.62±0.168µg/mL for control). Colostrum density, milk yield, and milk composition were similar between treatments. β-Carotene tended to increase milk protein content from 2.90 to 2.96% and to decrease the proportion of primiparous cows with a milk fat to protein ratio >1.5 from 22.6 to 6.4%. The proportion of primiparous and multiparous cows with difficult calving, metritis, progesterone >1 ng/mL at 21 d and at 42 d in lactation, % conception at first service, and % pregnancy at 90 and 150 d in lactation were similar between treatments. A trend for decreased incidence of somatic cell count >200,000 cells/mL was present in multiparous cows supplemented with β-carotene (38.9% vs. 28.1%). β-Carotene was associated with a reduction in the proportion of multiparous cows with retained placenta 12 h postpartum from 29.9 to 21.7%; time of placenta release was 392 min (340 to 440) for β-carotene and 490 min (395 to 540) for control (median and 95% confidence interval). For primiparous cows, placenta release was not affected by β-carotene (incidence was 15.4%). The intervals from calving to first estrus, to first service, and to conception were not affected by β-carotene supplementation in either parity. However, independent of treatment, cows with improved reproductive efficiency had increased postpartum β-carotene concentration in plasma. The prepartum supplementation of β-carotene increased plasma concentration around calving. No response in milk yield or reproductive performance was detected. Beta-carotene supplementation was associated with a lower incidence of retained placenta in multiparous cows.

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Section: Introductionmentioning

confidence: 98%

Supplementation of prepartum dairy cows with β-carotene

Oliveira

Guerreiro

Morais

et al. 2015

Journal of Dairy Science

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“…The cleavage activity of β CO was also reported into the ovary of bovine (Sklan, 1983; Schweigert et al., 1988). Also, a family of carotene‐cleaving enzymes has been described (von Lintig and Vogt, 2004; Wyss, 2004), and recently, we reported the cDNA sequence of bovine β CO and its expression in gonadal tissues, and we discussed its importance in vitamin A formation at this sites (Morales et al., 2006).…”

Section: Introductionmentioning

confidence: 99%

Differences in expression and activity of β,β′‐carotene‐15,15′‐ oxygenase in liver and duodenum of cattle with yellow or white fat

Morales¹,

González²,

Varela‐Echavarría³

et al. 2007

Animal Physiology Nutrition

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Pasture-fed cattle show yellow pigmentation of their fat due to beta-carotene stored in this tissue. beta,beta'-Carotene-15,15'-oxygenase (betaCO) is an enzyme expressed in different tissues, and it cleaves beta-carotene into retinal. We compared the expression and activity of betaCO in duodenum and liver of cattle with pigmented or non-pigmented fat. In the duodenum, in situ hybridizations showed expression of betaCO in epithelial cells and crypts of the mucosa that was similar in animals from pigmented and non-pigmented fat; liver showed diffuse signal at lobules, but pigmented animals showed higher signals near the portal space. Analyses by real-time reverse-transcription polymerase chain reaction also showed amplification of mRNA for betaCO in duodenum and liver, with no difference between pigmented or non-pigmented animals. Enzyme activity was similar in the duodenum, but pigmented animals had higher enzyme activity (p = 0.004) in liver. Cattle with pigmented fat had higher expression and activity of betaCO in liver, but its level was not high enough to prevent the storage of beta-carotene in adipose tissues.

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Assimilation and Conversion of Dietary Vitamin A into Bioactive Retinoids

Harrison

Seña's

2015

The Retinoids

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Cloning of the Bovine β-Carotene-15,15’-Oxygenase and Expression in Gonadal Tissues

Cited by 7 publications

References 41 publications

Supplementation of prepartum dairy cows with β-carotene

Supplementation of prepartum dairy cows with β-carotene

Differences in expression and activity of β,β′‐carotene‐15,15′‐ oxygenase in liver and duodenum of cattle with yellow or white fat

Assimilation and Conversion of Dietary Vitamin A into Bioactive Retinoids

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