Cloning of the HSP70 gene in barnacle larvae and its expression under hypoxic conditions

Molecular cloning and characterization of a heat shock protein 70 gene in swimming crab (Portunus trituberculatus)

“…Significant differences across control are indicated with an asterisk at P < 0.05, and with two asterisks at P < 0.01. significant increase after 12 h with the maximum at 48 h in M. galloprovincialis, the maximum at 8 h in Argopecten irradians challenged by Vibrio anguillarum and so on. In crustaceans, Hsp70 has been reported as a molecular chaperone in Balanus amphitrite, M. rosenbergii, Fenneropenaeus chinensis and Tigriopus japonicus [11,13,20,21]. The sequence and evolution analysis has also been performed in the hydrothermal crabs and shrimps [16,17].…”

“…Several members of the HSP70 family have been identified from various Crustacea [11][12][13][14][15][16][17][18][19][20][21]. Well-recognized members of the HSP70 multigenic family are two closed cytosolic forms which coexist inside Eukaryota: cognate Hsc70 and inducible Hsp70 [22].…”

Cloning and expression analysis of heat shock cognate 70 gene promoter in tiger shrimp (Penaeus monodon)

“…In addition to heat shock, the expression of HSP70 has also been reported to be induced under various stress conditions such as pathogen infection, amino acid analog (Mosser et al, 1988;Williams and Morimoto, 1990), heavy metal ions (Mosser et al, 1988;Yamuna et al, 2000;Boone and Vijayan, 2002), hypoxic condition (Cheng et al, 2003) and osmotic stress (Spees et al, 2002). Adenovirus ElA product can activate hsp70 promoter (Kraus et al, 1992) and baculovirus infection can also activate the Drosophila heat shock 70 promoter (Crouch and Passarelli, 2005).…”

Molecular cloning and expression of two HSP70 genes in the prawn, Macrobrachium rosenbergii

“…To our knowledge, it had been reported that expression of HSP70 was transiently induced by heavy metals (Hg and Cu) in a tissuespecific manner in freshwater prawn Macrobrachium mal-colmsonii (Yamuna et al 2000); using homologous complementary deoxyribonucleic acid (cDNA) probes, levels of HSP70 messenger ribonucleic acid (mRNA) in the lobster Homarus americanus was characterized during hypo-and hyperosmotic stress (Spees et al 2002); and a heat-inducible HSP70 was identified in the deep-sea vent shrimp Rimicaris exoculata (Ravaux et al 2003). Cheng et al cloned the HSP70 gene in barnacle larvae and characterized its expression under hypoxic conditions, which was the first report on the HSP70 gene sequence in crustaceans (Cheng et al 2003). In prawns, HSP70 and HSC70 gene structure has not been reported to date.…”