Cloning of the mismatch recognition protein MSH2 from zebrafish (Danio rerio) and its developmental stage-dependent mRNA expression

Yeh, Fu-Lung; Wang, Shi‐ya; Hsu, Lung-Ying; Wang, Dar-Yi; Hsu, Todd

doi:10.1016/j.bbaexp.2004.08.005

Cited by 14 publications

(9 citation statements)

References 22 publications

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“…This aspect allows zebrafish to be a suitable organism for use as a model for several human diseases (16 Photochemistry and Photobiology, 2009, 85: 220-226 zebrafish liver tumor possesses general molecular hallmarks of human cancer, showing deregulation in a large number of genes coding for proteins involved in cell cycle ⁄ proliferation, apoptosis, DNA replication and repair, and others. Many conserved components of different pathways from the DNA repair system have been described for zebrafish (12,17,18). Additionally, Sussman (19) reported that this species is highly competent in repairing UV radiation-induced damage.…”

Section: Introductionmentioning

confidence: 99%

Time‐course Expression of DNA Repair‐related Genes in Hepatocytes of Zebrafish (Danio rerio) After UV‐B Exposure

Sandrini

Trindade

Nery

et al. 2009

Photochem & Photobiology

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The objective of this study was to evaluate the time-course effects of UV-B exposure on expression of genes involved in the DNA repair system of zebrafish (Danio rerio) hepatocytes, a highly competent species in terms of damage repair induced by UV radiation. For gene expression analysis (RT-PCR), cells were exposed to 23.3 mJ cm )2 UV-B, which was the dose that affected viable cell number (reduction of 30% when compared with the control group) and produced no visual alteration on cell morphology. The early response observed (6 h) showed induction in the expression of the CDKI gene (cyclin-dependent kinase inhibitor) and genes related to DNA damage repair (mainly XPC and DDB2), while the late response observed (24 h) was more related to up-regulation of p53 and genes involved in cell cycle arrest (gadd45a, cyclinG1). In all times analyzed, the antiapoptotic gene Bcl-2 was down-regulated. Another interesting result observed was the up-regulation of the Apex-1 gene after UV-B exposure, which could indicate the induction of oxidative lesions in the DNA molecule. In conclusion, these results demonstrate an activation of the DNA repair system in hepatocytes of zebrafish exposed to UV-B radiation, mainly involving the participation of p53.

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Section: Introductionmentioning

confidence: 99%

Time‐course Expression of DNA Repair‐related Genes in Hepatocytes of Zebrafish (Danio rerio) After UV‐B Exposure

Sandrini

Trindade

Nery

et al. 2009

Photochem & Photobiology

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“…Amino acid alignment showed the existence of an N‐terminal mismatch binding domain and four C‐terminal nucleotide binding sites in the bacterial MutS as well as in zebrafish and other eukaryotic MSH2/MSH6 proteins . Direct exposure of purified yeast MutSα to Cd 2+ was shown to impair both the mismatch searching and the ATPase activities due to nonspecific binding of Cd 2+ to thiol‐containing residues and electronegative side chains of histidine in recombinant yeast MutSα .…”

Section: Discussionmentioning

confidence: 99%

“…Zebrafish ( Danio rerio ) embryo has been extensively used as a model organism for studying development‐regulated gene expression in vertebrates as well as for assessing the hazardous effects of chemical substances . We have cloned both MSH2 and MSH6 from zebrafish, and mRNAs encoding these two mismatch binding factors were found to express at high levels in zebrafish at early embryonic stages . Our previous studies also indicated the ability of Cd 2+ to inhibit msh2/msh6 expression via reactive oxygen species (ROS) as signaling molecules .…”

Section: Introductionmentioning

confidence: 99%

Comparative Effects of Mercury(II) and Cadmium on MutS Homolog 6(MSH6)-Mediated DNA Mismatch Binding Activities in Zebrafish (Danio rerio) Embryos

Sung

Huang

et al. 2015

J Biochem Mol Toxicol

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MutS homolog 6 (MSH6) of the MSH2-MSH6 complex binds simple mispairs and small insertion-deletion loops (IDL) then initiates DNA mismatch repair in eukaryotes. We have shown the ability of Cd(2+) to downregulate msh2/msh6 expression in zebrafish embryos via oxidative stress. This study explored the effects of Cd(2+) and Hg(2+) on MSH6-mediated mismatch binding activities. MSH6-mediated G-T and IDL-specific binding activities were significantly inhibited at similar levels after exposing zebrafish embryos at 1 h postfertilization) to HgCl2 or CdCl2 at 1.0 to 2.5 μM for 9 h, but MSH6 synthesis was found to be less sensitive to Hg(2+) than to Cd(2+) . Real-time RT-PCR and in situ hybridization also detected a weaker susceptibility of MSH gene transcription to Hg(2+) . The weaker response of MSH gene activities to Hg(2+) correlated with the lower oxidative stress-inducing potential of Hg(2+) . Hence, Hg(2+) targets mismatch sensing capacity at protein function rather than at transcription level.

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“…Eukaryotic mismatch repair is similar and includes several nuclear‐encoded MutS and MutL homologues: MutSα (MSH2/MSH6, MSH stands for MutS homolog) recognizes single base‐base mismatches and one or two base insertion/deletions, whereas MutSβ (MSH2/MSH3) recognizes insertion/deletion mismatches containing two or more extra bases (reviewed by Abdelnoor et al ., 2006; Larrea, Lujan & Kunkel, 2010). MSH2 and MSH6 of zebrafish were cloned (Yeh et al ., 2003, 2004) but their function in the stability of the mt genome of zebrafish has not been reported yet. Further studies should explore whether teleost MutS and MutL homologues are responsible for the stability of the mt genome, and whether these genes in Antarctic notothenioids have normal functions.…”

Section: Discussionmentioning

confidence: 99%

The complete mitochondrial genome of the mackerel icefish, Champsocephalus gunnari (Actinopterygii: Channichthyidae), with reference to the evolution of mitochondrial genomes in Antarctic notothenioids

Lin

Kao

2012

Zoological Journal of the Linnean Society

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The mackerel icefish (Champsocephalus gunnari Lönnberg, 1905) is a ray‐finned fish living in the Southern Ocean around Antarctica. We sequenced the complete mitochondrial (mt) genome of the mackerel icefish and a segment from cytochrome b to the control region (CR) in 32 individuals. The mt genome of the mackerel icefish was rearranged, containing two nicotinamide adenine dinucleotide (reduced form) dehydrogenase subunit 6 (ND6), two tRNAGlu, and two CRs. However, variations in numbers of ND6 and tRNAGlu were observed amongst individuals. These variations included type 1 (containing two ND6 and two tRNAGlu), type 2 (containing one ND6, one incomplete ND6, and one tRNAGlu), and type 3 (containing one ND6 and one tRNAGlu). The gene orders of types 1 and 2, and variations in numbers of ND6 and tRNAGlu were not previously found in any Antarctic notothenioids, whereas type 3 is the same as that of Racovitzia glacialis. Phylogenetic analyses of CR DNA sequences showed that duplicated CRs of the same species formed a monophyletic group, suggesting that duplication of CRs occurred in each species. The frequent duplication of mt genomes in Antarctic notothenioids is an unusual feature in vertebrates. We propose that interspecific hybridization and impairment of mismatch repair might account for the high frequency of gene duplications and rearrangement of mt genomes in Antarctic notothenioids.

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Cloning of the mismatch recognition protein MSH2 from zebrafish (Danio rerio) and its developmental stage-dependent mRNA expression

Cited by 14 publications

References 22 publications

Time‐course Expression of DNA Repair‐related Genes in Hepatocytes of Zebrafish (Danio rerio) After UV‐B Exposure

Time‐course Expression of DNA Repair‐related Genes in Hepatocytes of Zebrafish (Danio rerio) After UV‐B Exposure

Comparative Effects of Mercury(II) and Cadmium on MutS Homolog 6(MSH6)-Mediated DNA Mismatch Binding Activities in Zebrafish (Danio rerio) Embryos

The complete mitochondrial genome of the mackerel icefish, Champsocephalus gunnari (Actinopterygii: Channichthyidae), with reference to the evolution of mitochondrial genomes in Antarctic notothenioids

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