Cloning, tissue distribution and nutritional regulation of a fatty acyl Elovl4-like elongase in mud crab, Scylla paramamosain (Estampador, 1949)

Lin, Zhideng; Hao, Meilin; Huang, Yi‐Sheng; Zou, Weiguang; Hua, Rong; Wen, Xiaobo

doi:10.1016/j.cbpb.2017.12.010

Cited by 30 publications

(29 citation statements)

References 49 publications

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“…The tunicate C. intestinalis has an Elovl4, but appears to lack other PUFA elongases such as Elovl2/5. While still fragmented information, our current knowledge of the PUFA elongase repertoire in invertebrates suggests that, while the presence of Elovl2/5 is more restricted, Elovl4 has a more widespread distribution and appears as a distinct gene in cnidarians (Surm et al 2018), molluscs (Monroig et al 2017) and crustaceans (Lin et al 2018). Interestingly, the phylogenetic analysis appears to delineate the A. queenslandica protein Elovl1/2/4/5/7 as a basal sequence of the Elovl2/5 and Elovl4 found in more recently emerged invertebrate groups.…”

Section: Discussionmentioning

confidence: 99%

“…While the contribution of bacteria towards such elongation products could not be determined, it is now known that crustaceans possess Elovl genes that may account for some of the elongation activities reported in the studies above. Recently, an Elovl4 from the mud crab S. paramamosain has been investigated (Lin et al 2018). The S. paramamosain Elovl4, isolated from hepatopancreas, encodes a protein of 338 amino acids with all common features of Elovl protein family members (Jakobsson et al 2006).…”

Section: Crustaceansmentioning

confidence: 99%

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Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

2018

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Invertebrates represent a large proportion of the biomass in aquatic ecosystems, particularly in the ocean. In recent years, invertebrates have been well-consolidated models in evolutionary developmental biology research and, consequently, genomic and transcriptomic sequence databases from a plethora of species across the animal kingdom have become available. This has provided an excellent source of evidence confirming that invertebrates operate endogenous mechanisms for PUFA production. The present paper reviews the current knowledge of gene complement and, where possible, the function of desaturases and elongases with pivotal roles in PUFA biosynthesis of aquatic invertebrates. More specifically, this review covers three major enzyme types, namely ωx desaturases, front-end desaturases and elongases, that have been characterised from species of sponges, cnidarians, molluscs, annelids, crustaceans, rotifers, echinoderms and non-vertebrate chordates (amphioxus and sea squirt). These studies have shown that invertebrates operate alternative and unusual pathways of PUFA biosynthesis involving gene families with complex phylogeny and functional diversity. Consequently, research in this area provides potentially valuable molecular tools in the form of genes that can be used in the biotechnological production of n-3 products.

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Section: Discussionmentioning

confidence: 99%

Section: Crustaceansmentioning

confidence: 99%

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

2018

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“…Key enzymes involved in LC-PUFA biosynthesis in mud crab have been cloned, including Δ6 FAD-like (52) and ELOVL4-like (53) . It is well established that ELOVL4 effectively elongates C 22 PUFA to C 24 PUFA and has the potential to participate in the production of DHA in fish (54,55) .…”

Section: Discussionmentioning

confidence: 99%

Dietary lipid and n-3 long-chain PUFA levels impact growth performance and lipid metabolism of juvenile mud crab, Scylla paramamosain

et al. 2020

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An 8 weeks feeding trial was conducted to evaluate the effects of dietary n-3 LC-PUFA levels on growth performance, tissue fatty acid profiles and relative expression of genes involved in lipid metabolism of mud crab (Scylla paramamosain). Ten isonitrogenous diets were formulated to contain five n-3 LC-PUFA levels at 7 % and 12 % dietary lipid levels. Highest weight gain and specific growth rate were observed in crabs fed the diets with 19.8 and 13.2 mg g-1 n-3 LC-PUFA at 7 % and 12 % lipid, respectively. Moisture and lipid contents in hepatopancreas and muscle were significantly influenced by dietary n-3 LC-PUFA at the two lipid levels. The DHA, EPA, n-3 LC-PUFA contents and n-3/n-6 PUFA ratio in hepatopancreas and muscle significantly increased as dietary n-3 LC-PUFA levels increased at both lipid levels. The expression levels of Δ6 FAD and ACO in hepatopancreas increased significantly, and expression levels of FAS, CPTⅠ and HSL were down-regulated, with increased dietary n-3 LC-PUFA regardless of lipid level. Based on weight gain, the n-3 LC-PUFA requirements of S. paramamosain were estimated to be 20.1 mg g-1 and 12.7 mg g-1 of diet at 7 % and 12 % dietary lipid, respectively. Over all, dietary lipid level influenced lipid metabolism, and purified, high-lipid diets rich in palmitic acid reduced the n-3 LC-PUFA requirement of juvenile mud crab.

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“…In the present study, key genes involved in LC-PUFA biosynthesis, such as fad, elovl4, elovl, srebp1, hnf4a and rxr, were significantly down-regulated when dietary lipid level increased from 5•8 to 15•1 %, which was in agreement with previous studies in teleost fish when subjected to similar experimental conditions (38) . Moreover, some studies in crustaceans showed that fad and elovl4 expression were inhibited by the level of dietary fish oil (39)(40)(41) . This result may suggest that high dietary fish oil could inhibit the synthesis of LC-PUFA in hepatopancreas of crab or, alternatively, that low dietary LC-PUFA activates endogenous production of these key fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

et al. 2019

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The regulation of lipogenesis and lipolysis mechanisms related to consumption of lipid has not been studied in swimming crab. The aims of the present study were to evaluate the effects of dietary lipid levels on growth, enzymes activities and expression of genes of lipid metabolism in hepatopancreas of juvenile swimming crab. Three isonitrogenous diets were formulated to contain crude lipid levels at 5·8, 9·9 and 15·1 %. Crabs fed the diet containing 15·1 % lipid had significantly lower growth performance and feed utilisation than those fed the 5·8 and 9·9 % lipid diets. Crabs fed 5·8 % lipid had lower malondialdehyde concentrations in the haemolymph and hepatopancreas than those fed the other diets. Highest glutathione peroxidase in haemolymph and superoxide dismutase in hepatopancreas were observed in crabs fed 5·8 % lipid. The lowest fatty acid synthase and glucose 6-phosphate dehydrogenase activities in hepatopancreas were observed in crabs fed 15·1 % lipid, whereas crabs fed 5·8 % lipid had lower carnitine palmitoyltransferase-1 activity than those fed the other diets. Crabs fed 15·1 % lipid showed lower hepatopancreas expression of genes involved in long-chain-PUFA biosynthesis, lipoprotein clearance, fatty acid uptake, fatty acid oxidation, lipid anabolism and lipid catabolism than those fed the other diets, whereas expression of some genes of lipoprotein assembly and fatty acid oxidation was up-regulated compared with crabs fed 5·8 % lipid. Overall, high dietary lipid level can inhibit growth, reduce antioxidant enzyme activities and influence lipid metabolic pathways to regulate lipid deposition in crab.

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Cloning, tissue distribution and nutritional regulation of a fatty acyl Elovl4-like elongase in mud crab, Scylla paramamosain (Estampador, 1949)

Cited by 30 publications

References 49 publications

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

Dietary lipid and n-3 long-chain PUFA levels impact growth performance and lipid metabolism of juvenile mud crab, Scylla paramamosain

Effects of dietary lipid level on growth, fatty acid profiles, antioxidant capacity and expression of genes involved in lipid metabolism in juvenile swimming crab, Portunus trituberculatus

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