Clostridium butyricum Prevents Dysbiosis and the Rise in Blood Pressure in Spontaneously Hypertensive Rats

Abstract

Hypertension is accompanied by dysbiosis and a decrease in the relative abundance of short-chain fatty acid (SCFA)-producing bacteria. However, there is no report to examine the role of C. butyricum in blood pressure regulation. We hypothesized that a decrease in the relative abundance of SCFA-producing bacteria in the gut was the cause of spontaneously hypertensive rats (SHR)-induced hypertension. C. butyricum and captopril were used to treat adult SHR for six weeks. C. butyricum modulated SHR-induced dysbiosis and significantly reduced systolic blood pressure (SBP) in SHR (p < 0.01). A 16S rRNA analysis determined changes in the relative abundance of the mainly SCFA-producing bacteria Akkermansia muciniphila, Lactobacillus amylovorus, and Agthobacter rectalis, which increased significantly. Total SCFAs, and particularly butyrate concentrations, in the SHR cecum and plasma were reduced (p < 0.05), while C. butyricum prevented this effect. Likewise, we supplemented SHR with butyrate for six weeks. We analyzed the flora composition, cecum SCFA concentration, and inflammatory response. The results showed that butyrate prevented SHR-induced hypertension and inflammation, and the decline of cecum SCFA concentrations (p < 0.05). This research revealed that increasing cecum butyrate concentrations by probiotics, or direct butyrate supplementation, prevented the adverse effects of SHR on intestinal flora, vascular, and blood pressure.

Metagenomic analysis reveals the composition and function of intestinal microbiota were affected by different sampling methods and digestive tract segments in monogastric and polygastric animals

“…In the fecal samples, the species of more abundant bacteria in the fecal samples (LDA > 2) were Lactobacillus hayakitensis, Lactobacillus equi, Lactobacillus amylovorus, Lactobacillus salivarius, Lactobacillus gastricus, Lactobacillus hamsteri and Lactobacillus acidophilus belonging to the Lactobacillus, which was the predominant microbes in the horse [31]. Lactobacillus amylovorus, a short-chain fatty acid (SCFA)-producing bacteria [32], has been reported to reduce weight and fat content in humans [33]. What's more, Lactobacillus amylovorus is a kind of Lactobacillus that can e ciently utilize starch, as a food and feed additive, it has been widely used for mildew prevention and antibacterial, bacteriostasis, and enzyme production [34].…”

Gut microbiome and metabolome in aneurysm rat with hypertension after ginsenoside Rb1 treatment

“…In a study, the ginsenoside Rb1 group exhibited marked increases in the genera Clostridium, Roseburia, Ruminococcus, and Treponema (Wang et al, 2021). Another group showed that losartan, in addition to reducing blood pressure, increased the levels of Aristophanes, Lactobacillus, and Butyric acidophilus, while decreasing Ruminococcaceae, Streptococcus, and Turbotobacteria (Luo et al, 2023). Furthermore, a survey of the fecal microbiota demonstrated a positive association between specific Vibrio lachnosus spp.…”

Bioactive Components in Fruit Interact with Gut Microbes

“…Ruminococcus bromii could produce butyrate in the colon by fermenting resistant starch [136]. The latest investigations suggest that butyrate prevents hypertension and inflammation [137]. Several recent clinical studies support that SCFAs play a beneficial role in the prevention and treatment of human diseases, such as regulation of cardio-metabolic outcomes [130], improvement of gut barrier integrity [138], regulating cardiac function [139], modulation of glucose and lipid metabolism [140], and mediation of the immune system promoting insulin secretion [141], increasing energy expenditure and reducing fat accumulation [142] and anti-inflammatory response [143].…”