Clostridium perfringens Epsilon Toxin: Structural and Mechanistic Insights

Popoff, Michel R.; Stiles, Bradley G.; Poulain, Bernard

doi:10.1007/978-94-007-6449-1_9

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“…ETX is the causative agent of enterotoxaemia, caused by C. perfringens toxinotype D, which is a fatal disease responsible for death in sheep worldwide (Popoff, 2011; Popoff et al . 2016). Antibodies produced against C. perfringens render vaccination against its toxins as a prophylactic measure (Gonçalves et al .…”

Section: Introductionmentioning

confidence: 99%

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Bacillus toyonensisBCT‐7112^Ttransient supplementation improves vaccine efficacy in ewes vaccinated againstClostridium perfringensepsilon toxin

Santos

Ferreira

Maubrigades

et al. 2020

J. Appl. Microbiol.

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Aim The aim of the present study was to examine the vaccine immune response in ewes supplemented with Bacillus toyonensis BCT‐7112T during a period of 5‐day supplementation before vaccination against a recombinant Clostridium perfringens epsilon toxin (rETX). Methods and Results Ewes were vaccinated with 200 µg of rETX adjuvanted with 10% aluminium hydroxide. The treat group was orally supplemented with B. toyonensis BCT‐7112T (3 × 108 viable spores) for 5 days prior to the first and second vaccination. Ewes supplemented with B. toyonensis BCT‐7112T showed higher neutralizing antibody titres than the non‐supplemented ewes (P < 0·05), with an increase in serum levels for total IgG anti‐rETX by 3·2‐fold (P < 0·0001), and for both IgG isotypes IgG1 and IgG2 by 2·1‐fold and 2·3‐fold (P < 0·01), respectively, compared with the control group. The peripheral blood mononuclear cells of ewes in the supplemented group had a higher (P < 0·05) cytokine mRNA transcription levels for IL‐2 (6·4‐fold increase), IFN‐γ (2·9‐fold increase) and transcription factor Bcl6 (2·3‐fold increase) compared with the control group. Conclusion We conclude that a 5 days of supplementation with B. toyonensis BCT‐7112T prior vaccination is sufficient to significantly improve the humoral immune response of ewes against C. perfringens recombinant ETX vaccine. Significance and Impact of the study These findings open a new perspective in the utilization of B. toyonensis BCT‐7112T as an immunomodulator since a 5 days period of probiotic supplementation is sufficient to improve the vaccine immune response.

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Section: Introductionmentioning

confidence: 99%

“…2009; Popoff et al . 2016). Recombinant vaccines against ETX have emerged as alternatives to conventional toxoids (formaldehyde‐inactivated toxins) because they offer advantages in terms of both efficacy and process safety.…”

Section: Introductionmentioning

confidence: 99%

Bacillus toyonensisBCT‐7112^Ttransient supplementation improves vaccine efficacy in ewes vaccinated againstClostridium perfringensepsilon toxin

Santos

Ferreira

Maubrigades

et al. 2020

J. Appl. Microbiol.

View full text Add to dashboard Cite

show abstract

Clostridium perfringens Epsilon Toxin: Structural and Mechanistic Insights

Cited by 1 publication

References 96 publications

Bacillus toyonensisBCT‐7112^Ttransient supplementation improves vaccine efficacy in ewes vaccinated againstClostridium perfringensepsilon toxin

Bacillus toyonensisBCT‐7112^Ttransient supplementation improves vaccine efficacy in ewes vaccinated againstClostridium perfringensepsilon toxin

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Clostridium perfringens Epsilon Toxin: Structural and Mechanistic Insights

Cited by 1 publication

References 96 publications

Bacillus toyonensisBCT‐7112Ttransient supplementation improves vaccine efficacy in ewes vaccinated againstClostridium perfringensepsilon toxin

Bacillus toyonensisBCT‐7112Ttransient supplementation improves vaccine efficacy in ewes vaccinated againstClostridium perfringensepsilon toxin

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Product

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Bacillus toyonensisBCT‐7112^Ttransient supplementation improves vaccine efficacy in ewes vaccinated againstClostridium perfringensepsilon toxin

Bacillus toyonensisBCT‐7112^Ttransient supplementation improves vaccine efficacy in ewes vaccinated againstClostridium perfringensepsilon toxin