Closure of the Uterine Lumen at Implantation in Senescent Golden Hamsters

Thorpe, L. W.; Connors, Thomas J.; Soderwall, A. L.

doi:10.1530/jrf.0.0390029

Cited by 10 publications

(4 citation statements)

References 11 publications

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“…The same situation was noted in the old rat [66]. In senescing golden hamsters, both closure and implantation were deferred by approximately 12 h and luminal closure was found only around the implanting embryos [67]. Where embryos were not present, the lumen remained open and branched and luminal epithelial cells had tall, regular microvilli which did not contact microvilli from the opposing epithelial border.…”

Section: Humanmentioning

confidence: 58%

Determinants of uterine aging: lessons from rodent models

Kong

Zhang

Chen

et al. 2012

Sci. China Life Sci.

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The uterus is an indispensable organ for the development of a new life in eutherian mammals. The female mammalian reproductive capacity diminishes with age. In this respect, the senescence of uterine endometrium is convinced to contribute to this failure. This review focuses on the physiological function of the uterus and the related influence of aging mainly in rodent models. A better understanding of the underlying mechanisms governing the process of uterine aging is hoped to generate new strategies to prolong the reproductive lifespan in humans. . More importantly, this phenomenon of continuous decline of fecundity with age has also been confirmed in women over 35 years old [10][11][12]. The success of reproduction in eutherian mammals requires the uterus, a unique organ in the viviparity compared to other organisms ensuring full development of the new life in the maternal body [13]. The endometrial environment of uteri is proved to be one of the critical aspects to determine the reproductive capacity [14] and uterine factor has been proved to be critical for the decline of fecundity in both rodents and humans [4,15]. Most of the available information on uterine aging derives from studies on rats and mice [16]. In these species, life span is short and aging animal model can be easily established. This review focuses on the physiological function of the uterus and the related influence of aging mainly in rodent models. determinants, uterine aging, rodent models

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Section: Humanmentioning

confidence: 58%

Determinants of uterine aging: lessons from rodent models

Kong

Zhang

Chen

et al. 2012

Sci. China Life Sci.

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show abstract

“…Where implantation sites were found, an attachment reaction was seen, and it was suggested that an impaired epithelial response to estrogen might be one of the causes of implantation failure in aged mice. In senescing golden hamsters, both closure and implantation were delayed by approximately 12 hours and luminal closure was found only in the vicinity of implanting embryos (Thorpe et al, 1974). Where embryos were not present, the lumen remained open and branched and luminal epithelial cells had tall, regular microvilli which did not contact microvilli from the opposing epithelial border.…”

Section: Age-related Changes In the Endometrium Epitheliummentioning

confidence: 99%

Characteristics of uterine aging

Mulholland

Jones

1993

Microscopy Res & Technique

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Uterine aging is in part responsible for a decline in fecundity which begins in middle age in most mammals. Characteristics of uteri from a variety of animals in middle age and old age are reviewed and the factors which may be responsible for this decline discussed. These include age-related changes in the hypothalamus, pituitary, and ovaries; loss of number or function of steroid hormone receptors; morphological changes in the uterine epithelium; the accumulation of collagen fibrils in the uterine stroma; and loss or impairment of the decidual response. The ultrastructural morphology of uterine epithelial, stromal, and decidual tissue from 4 month old and 18 month old Fischer 344 rats is presented and compared.

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“…This aids closure of the total uterine lumen to achieve close apposition between the blastocyst and apical surface of UECs (Lindsay & Murphy 2005, 2006. It is important to note that luminal closure occurs all along the uterine horn and not just in the implantation chamber (Thorpe et al 1974, Png & Murphy 2000. In previous studies, syntaxin 3 has been shown to interact with sodium channels in epithelial cells, where they regulate intrinsic properties and cell-surface expressions of channels (Saxena et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Membrane trafficking directed by VAMP2 and syntaxin 3 in uterine epithelial cells

et al. 2020

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Luminal uterine epithelial cells (UEC) have a surge in vesicular activity during early uterine receptivity. It has been predicted these vesicles exit the UEC via exocytosis resulting in secretion and membrane trafficking. The present study investigated the changes in SNARE proteins VAMP2 (v-SNARE) and syntaxin 3 (t-SNARE) localisation, and abundance in UECs during early pregnancy in the rat. We found VAMP2 and syntaxin 3 are significantly higher on day 5.5 compared to day 1 of pregnancy. On day 5.5, VAMP2 is perinuclear and syntaxin 3 is concentrated in the apical cytoplasm compared to a cytoplasmic localisation on day 1. This change in localisation and abundance show VAMP2 and syntaxin 3 are involved in vesicular movement and membrane trafficking in UECs during early pregnancy. This study also investigated the influence of cytoskeletal disruption of microtubules and actin filaments on VAMP2 and syntaxin 3 in UECs grown in vitro since microtubules and actin influence vesicle trafficking. As expected, this study found disruption to microtubules with colchicine and actin with cytochalasin D impacted VAMP2 and syntaxin 3 localisation. These results suggest VAMP2 and syntaxin 3 are involved in the timely trafficking of vesicular membranes to the apical surface in UECs during early pregnancy, as are of microtubules and actin.

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Closure of the Uterine Lumen at Implantation in Senescent Golden Hamsters

Cited by 10 publications

References 11 publications

Determinants of uterine aging: lessons from rodent models

Determinants of uterine aging: lessons from rodent models

Characteristics of uterine aging

Membrane trafficking directed by VAMP2 and syntaxin 3 in uterine epithelial cells

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