Clotting factor activity in fresh frozen plasma after thawing with a new radio wave thawing device

Abstract

BACKGROUND Massive hemorrhage usually results in rapid need of blood products. Patients in need of fresh frozen plasma (FFP) might benefit from shorter thawing times using a novel radio wave device. So far, only one study on the prototype has been published. Activities of clotting factors after thawing FFP with the radio wave device and with a system using water cushions were compared. This is the first study analyzing the quality of FFP using the fully developed radio wave thawing device UFT100. STUDY DESIGN AND METHODS Thirty FFP units were thawed with the UFT100 and the Plasmatherm machine. Various clotting factors and inhibitors were analyzed before freezing, immediately after thawing, and after a 48‐hour storage period at +4°C. RESULTS After thawing, all factor activities were within normal ranges regardless of the thawing procedure. We observed significant differences in nearly all clotting factor activities regarding time as effector, whereas thawing with the Plasmatherm machine led to a significant decrease (>10%) only in factor V activity compared to the UFT100. CONCLUSIONS Immediately after rapid thawing with the UFT100, all FFP units contained adequate coagulation factor activities to maintain hemostatic activity. The UFT100 does not deteriorate FFP quality compared to a conventional system. Regardless of the thawing system, the postthaw refrigerated storage caused a decrease in several clotting factors and inhibitors (factors V, VIII, and IX; von Willebrand factor activity; protein S and C activity) and a significant increase of factor XI.

[Retracted] Correlation Analysis of DNA Methylation in the von Willebrand Factor Promoter Region and the Risk of Unexplained Recurrent Hemophilia: Systematic Review and Meta‐Analysis

“…Further study on the mechanism of synthesis, secretion, degradation, and clearance of vWF, as well as the relationship between the structure and function of vWF, will help to find a new way of hemostasis and prevention and treatment of blood thrombolysis [ 27 ]. vWF is a glycoprotein synthesized by vascular endothelial cells and bone marrow megakaryocytes, which plays an important role in both stage 1 and stage 2 hemostasis [ 28 ]. Lack of vWF will lead to von Willebrand disease, and the overexpression of vWF plays an important role in thrombosis.…”

Evaluation of changes in coagulation factors in fresh frozen plasma during storage at -18°C for 5 weeks at Kisii Teaching and Referral Hospital

“…The study results indicated that, as the donated blood continues to be stored, there was a significant reduction in coagulation factors in fresh frozen plasma. A study done by (10), indicated that the coagulation factors decreased significantly after storage. But for better fresh frozen plasma then, thawing at 37°C has a crucial impact on the quality of the plasma content in blood before transfusion.…”

Field‐expedient thawing of fresh‐frozen plasma

“…Alternatives to frozen product exist (or are on the horizon), including liquid, never‐frozen plasma, dried plasma, and whole blood, but FFP remains the most commonly available version due to its cost effectiveness and long shelf life. Additionally, multiple alternatives to water bath thawing have been developed or are under investigation for clinical usage . It remains to be seen if any of these will present as superior solutions for austere environments, but it should be noted that, even as far back as the 1970s, microwave ovens were investigated as possible rapid thawing alternatives with only minor denaturing effects observed …”

“…Additionally, multiple alternatives to water bath thawing have been developed or are under investigation for clinical usage. [10][11][12] It remains to be seen if any of these will present as superior solutions for austere environments, but it should be noted that, even as far back as the 1970s, microwave ovens were investigated as possible rapid thawing alternatives with only minor denaturing effects observed. [13][14][15] This study was based on the premise that frozen plasma should be made available as soon as possible and thawed using limited resources.…”