Clove and eugenol in noncytotoxic concentrations exert immunomodulatory/anti-inflammatory action on cytokine production by murine macrophages

Bachiega, Tatiana Fernanda; Sousa, João Paulo Barreto de; Bastos, Jairo Kenupp; Sforcin, José Maurício

doi:10.1111/j.2042-7158.2011.01440.x

Cited by 145 publications

(94 citation statements)

References 30 publications

(34 reference statements)

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“…In general, our findings here appear to contrast with those from an in vitro study by Bachiega et al (2012) that showed a presence of clove oil could inhibit IL-1, IL-6, and IL-10 production by LPS-stimulated macrophages after a 24-h culture. In contrast, an in vivo study by Grespan et al (2012) indicated that eugenol treatment resulted in enhanced IL-10 cytokine production by cells at sites of inflammation in a murine model of collagen-induced arthritis.…”

Section: Discussioncontrasting

confidence: 56%

“…While some biologic effects from exposure to clove have been reported (and noted above), apart from the above-noted antiinflammatory effects, effects by clove or key constituents (like eugenol) on specific immune system components/mechanisms have only recently begun to be examined in detail (Bachiega et al, 2012;Grespan et al, 2012;Lee et al, 2007;Rodrigues et al, 2009;Yogalakshmi et al, 2010). As many of those studies have often yielded conflicting results, we undertook a series of studies that would hopefully provide some clarity regarding immunotoxicity/immunomodulatory effects of clove-associated materials.…”

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Dibazar

Fateh

Daneshmandi

2014

Journal of Immunotoxicology

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The present work sought to investigate potential suppressive effects on mouse macrophages by in vitro treatment with clove (Syzygium aromaticum) ethanolic extracted essential oil (containing eugenol) or its water-soluble extract. Using doses (ranging from 0.001-1000 mg/ml) of each material freshly prepared in the laboratory, cell survival and production of nitric oxide (NO), tumor necrosis factor (TNF)-, interleukin (IL)-6, and IL-12 by the treated cells (that in all cases also had received LPS stimulation) were measured. Results indicated that, except at doses !100 mg/ml, viability was unaffected in all groups. NO release by LPS-stimulated macrophages was generally significantly suppressed by either material; in contrast, low (i.e. 0.001-1 mg/ml) doses of either extract class appeared to enhance NO release by non-LPS (unstimulated)-treated macrophages. Among LPS-stimulated cells, TNF release was also significantly affected by each extract; the ethanolic extract was suppressive at all doses tested, while the aqueous material was so up to 1 mg/ml and then became stimulatory. In contrast, nearly every dose of either extract appeared to stimulate IL-6 release from the LPS-treated cells. Effects on IL-12 production were overall inconsistent; in general, the ethanolic extract tended to be stimulatory of production by the LPS-treated cells. The data for the aqueous material showed no discernable pattern of effect. The results suggest that clove extracts do not have a distinct cytotoxic activity, but do impart potential anti-and pro-oxidant effects in cells, depending on their concentrations and on the activation state of the macrophages themselves at the time of exposure to the extracts. The impact of the extracts on macrophage cytokine release also displays a pattern of dose-relatedness.

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Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Dibazar

Fateh

Daneshmandi

2014

Journal of Immunotoxicology

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“…Apparently, Th1 and Th2 cells react adversely to eugenol -while Th1 cytokines TNF-α and IL-2 production by nicotine-induced murine macrophages were down-regulated by eugenol, the generation of Th-2 cytokines IL-10 and TGF-β was activated [19]. On the other hand, administration of clove to mice did not exert any effect on the Th1/Th2 balance [20]. The anti-inflammatory effect of eugenol has been demonstrated on rats with carrageenan-induced inflammation that was markedly alleviated by eugenol containing diet [21].…”

Section: Discussionmentioning

confidence: 93%

Eugenol as a modulator of the immune dialogue between human mononuclears and colon cancer cells

Djaldetti¹,

Bessler²

2017

J Mol Pathophysiol

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Objective: Cloves (Syzygium aromaticum), the dried flower buds of a tree belonging to the Myrtaceae family have achieved a top place in herbal medicine. To its main active compound eugenol have been attributed definite anti-anaphylactic, anti-inflammatory, and even anticancer properties. It was the object of the study to elucidate the immunomodulatory potential of eugenol and its ability to alter the crosstalk between human peripheral blood mononuclear cells (PBMC) and cells from two human colon carcinoma lines. Materials and Methods: Eugenol, at concentrations of 3.75, 7.5, and 15 µg/ml, was added for 24 h to PBMC alone or to cells co-incubated with either HT-29 or RKO colon carcinoma cells. Subsequently, the generation of the following cytokines was examined: Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IFN-γ, IL-1ra, and IL-10. Results: Eugenol did not affect the capacity of unstimulated PBMC or that of cancer cells to produce any of the cytokines examined. However, when eugenol was added to PBMC co-incubated with HT-29 cells, the production of all cytokines was inhibited, except for IL-6 and IFNγ. The capacity for cytokine generation by PBMC co-incubated with RKO cells was reduced concerning TNF-α and IL-1β. Conclusions: The results indicate that eugenol exerts an immunomodulatory effect on PBMC to produce inflammatory cytokines when stimulated by colon carcinoma cells from the two lines examined. This effect has been shown to be cancer cell type dependent. The capacity of eugenol to interfere with the crosstalk between immune and colon cancer cells may further explain the restraining effect of this compound on cancer development.

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“…Propolis, p-coumaric and cinnamic acids stimulated IL-1b production and inhibited IL-6 and IL-10 generation by peritoneal macrophages [16]. Clove inhibited IL-1 b, IL-6 and IL-10 production, whereas eugenol did not affect IL-1b production but inhibited IL-6 and IL-10 production [17]. Baccharis dracunculifolia and caffeic acid stimulated IL-1b and inhibited IL-6 and IL-10 production [18].…”

Section: Discussionmentioning

confidence: 98%

Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes

Santiago¹,

Conti²,

Andrade³

et al. 2015

Biomedicine & Pharmacotherapy

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Clove and eugenol in noncytotoxic concentrations exert immunomodulatory/anti-inflammatory action on cytokine production by murine macrophages

Abstract: Clove exerted immunomodulatory/anti-inflammatory effects by inhibiting LPS action. A possible mechanism of action probably involved the suppression of the nuclear factor-κB pathway by eugenol, since it was the major compound found in clove extract.

Cited by 145 publications

References 30 publications

Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Immunomodulatory effects of clove (Syzygium aromaticum) constituents on macrophages:In vitroevaluations of aqueous and ethanolic components

Eugenol as a modulator of the immune dialogue between human mononuclears and colon cancer cells

Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes

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