Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes

Santiago, Karina Basso; Conti, Bruno José; Andrade, Bruna Fernanda Murbach Teles; Silva, Jonas Joaquim Mangabeira da; Rogez, Hervé; Crevelin, Eduardo José; Moraes, Luiz Alberto Beraldo; Veneziani, Rodrigo Cássio Sola; Ambrósio, Sérgio Ricardo; Bastos, Jairo Kenupp; Sforcin, José Maurício

doi:10.1016/j.biopha.2014.12.035

Cited by 33 publications

(27 citation statements)

References 26 publications

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“…The biological effect of the copaiba oil is specially attributed to some compounds with antiinflammatory action, such as -caryophyllene [5,10] and copalic acid [11]. In vitro studies suggest that caryophyllene is a dietary cannabinoid with important anti-inflammatory effects through the inhibition of cannabinoid type-2 receptor [10].…”

Section: Discussionmentioning

confidence: 99%

Ethanolic Extracts of Copaifera Multijuga Inhibits the Subcutaneous Growth of Ehrlich Carcionoma in Swiss Mice

Albiero¹,

Nery²,

Kelly³

et al. 2016

IOSR

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Oil-resin of Copaifera multijuga Hayne is popularly used due to its anti-inflammatory properties. In this study, we evaluate the effects of the bark ethanolic extract on Ehrlich tumor cells. For extract preparation, bark was removed from stem and chopped into smaller pieces. Most were dried and grounded. The first extract was made with hexane, followed by ethyl acetate. Absolute ethanol was added to the residue and extract was filtered and concentrated (EtOH). In vitro cytotoxic activity of the EtOH was analyzed on both spleen and tumor cells. For evaluation of in vivo activity, Ehrlich carcinoma bearing Swiss mice were daily treated with different concentration of EtOH during 30 days. Evaluation of in vitro cell viability showed that 1mg/ml extract is cytotoxic to spleen and tumor cells. Evaluation of in vivo tumor development showed that treatment with 200mg/kg of EtOH was able to reduce in 45.28% the tumor growth. Treatment for 7 days increased IL-12p70, IFN-γ and TNF-α production and for 14 days reduced the lymphoproliferative activity in both tumor bearing and mice healthy. Our results show that the ethanolic extract of copaiba bark affect Ehrlich tumor cells, reducing their cell viability in vitro as well as their development in vivo.

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Section: Discussionmentioning

confidence: 99%

Ethanolic Extracts of Copaifera Multijuga Inhibits the Subcutaneous Growth of Ehrlich Carcionoma in Swiss Mice

Albiero¹,

Nery²,

Kelly³

et al. 2016

IOSR

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“…The trees of the genus Copaifera are popularly known in Brazil as copaíba, with 26 species distributed throughout north and northeastern Brazil (Costa 2018). Copaiba oleoresin, which is extracted from the trunk of the tree and is rich in terpene compounds, including sesquiterpenes and diterpenes, is very popular as a medicinal plant compound in northern Brazil (Veiga and Pinto 2002;Santiago et al 2015;Silva et al 2017;Furtado et al 2018). The oleoresin of 17 species of Copaifera has been chemically studied (Veiga et al 2007;Gramosa et al 2010;Leandro et al 2012), and has been described as having anticancer activities (Gomes et al 2008), antiparasitic activity against Chagas disease (Izumi et al 2012), and anti-inflammatory and neuroprotective activity (Guimarães-Santos et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice

et al. 2019

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Copaifera multijuga, commonly known as copaiba, is popularly used in the form of tea for various conditions due to the presence of antioxidant substances in its composition, which protect cells against damage caused by free radicals. Its oleoresin is also used as an anti-inflammatory and antitumoral agent. The present study investigated the antioxidant effect of the ethanolic extract of copaiba stem bark on Swiss mice inoculated with solid Ehrlich tumors. Mice were inoculated subcutaneously with 1x106 Ehrlich’s tumor cells and treated via gavage with ethanolic extract of copaiba for thirty days, with doses varying between 100 and 200 mg kg-1. Biochemical analyses of enzymatic antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST)], non-enzymatic antioxidants [reduced glutathione (GSH) and ascorbic acid (ASA)], substances reactive to thiobarbituric acid (TBARS) and protein carbonylation (carbonyl) in different tissues were significantly affected. The extract administered at 200 mg kg-1 presented higher antioxidant capacity in the liver, increased CAT, GST, GSH and decreased TBARS, as well as increased CAT activity and protein carbonylation in brain tissue. The results showed that the copaiba extract was able to reverse the oxidative stress caused by solid Ehrlich tumor, probably due to the presence of antioxidant compounds, and had potential antineoplasic effect after a 30-day treatment.

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“…They are usually commercialized as capsules or crude oil (Veiga, Zunino, Calixto, Patitucci, & Pinto, ). Recently it was reported that Copaiba oleoresin also displays immunomodulatory activity (Santiago et al, ). The oleoresin obtained from the trunk of these species is chemically composed of resin acids and volatile compounds (Leandro et al, ).…”

Section: Introductionmentioning

confidence: 99%

Use of spinning band distillation equipment for fractionation of volatile compounds ofCopaiferaoleoresins for developing a validated gas chromatographic method and evaluating antimicrobial activity

Ribeiro

Arruda

Silva

et al. 2018

Biomedical Chromatography

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Copaifera is a tree that produces an oleoresin that has great historical and economic importance. These oleoresins display several pharmacological properties, such as antiinflammatory and antimicrobial, among others. The commercialization of Copaifera oleoresin occurs, in many cases, without any quality control, which facilitates its adulteration. Validated analytical methods can provide a safe quality control. In this work, the 800 Automatic Spinning Band Distillation equipment was used to perform the fractionation of the volatile oils obtained by hydrodistillation of Copaifera multijuga, C. paupera, C. Publifora and C. langsdorffii, aiming to isolate and purify the major compounds present in these oils. For purification, classical column chromatography was used, furnishing six isolated sesquiterpenes. The sesquiterpenes were used as standards in the development and validation of the method by GC-FID. The evaluated parameters were selectivity, linearity, precision, accuracy and robustness and they are all in accordance with ANVISA and International Conference on Harmonization guidelines. The developed method is reliable for the quantification of sesquiterpenes in Copaifera oleoresins. Both volatile oils and isolated sesquiterpenes had their minimum inhibitory concentration determined against strains of Gram-negative and Grampositive bacteria and yeasts. Copaifera langsdorffi oleoresin was the only one active against all of the evaluated microorganisms, displaying good antimicrobial potential.

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Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes

Cited by 33 publications

References 26 publications

Ethanolic Extracts of Copaifera Multijuga Inhibits the Subcutaneous Growth of Ehrlich Carcionoma in Swiss Mice

Ethanolic Extracts of Copaifera Multijuga Inhibits the Subcutaneous Growth of Ehrlich Carcionoma in Swiss Mice

Evaluation of the antioxidant potential of Copaifera multijuga in Ehrlich tumor-bearing mice

Use of spinning band distillation equipment for fractionation of volatile compounds ofCopaiferaoleoresins for developing a validated gas chromatographic method and evaluating antimicrobial activity

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