CMV-DNA detection in parenchymatous organs in cases of SIDS

Cecchi, Rossana; Bajanowski, Thomas; Kahl, Barbara C.; Wiegand, Peter

doi:10.1007/bf01246875

Cited by 24 publications

(11 citation statements)

References 18 publications

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“…If the results of DNA virus detection as previously reported [7,8] are added, viruses causing IP were detectable in 48% (Table2). Moreover, the investigation was successful in 25% of the SIDS cases compared to 8% of the non-SIDS cases.…”

Section: Discussionmentioning

confidence: 93%

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Detection of RNA viruses in sudden infant death (SID)

Bajanowski

Rolf

Jorch

et al. 2003

International Journal of Legal Medicine

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Continuing previous work, reverse transcriptase polymerase chain reaction approaches for the detection of RNA viruses (influenza A+B, parainfluenza virus 3, respiratory syncytial virus) were developed. A total of 118 infant deaths, including 13 cases of non-natural death (nND), 78 cases of natural death (ND) without morphological signs of interstitial pneumonia (IP), 27 cases showing IP (ND+IP), were investigated using frozen lung tissue ( N=100) and paraffin-embedded material ( N=18). In five of the autopsy cases (ND+IP) the influenza B virus genome could be detected and the other types of viruses were completely negative. Together with previous results (detection of adenoviruses and cytomegaloviruses) in the same groups, the frequency of virus detection in the cases with IP was 48% compared with 14% in the ND without IP and 7% in the nND. Significant differences in the frequency of virus detection were also obtained when the cases were divided in SIDS and non-SIDS. The results obtained indicate an association between IP and some viruses and support the hypothesis that respiratory virus infections could act as trigger in sudden infant death.

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Section: Discussionmentioning

confidence: 93%

“…Cases A total of 105 subsequent cases of sudden infant death (natural deaths ND) and 13 non-natural deaths (nND) were investigated by full autopsy using a standard autopsy protocol [7,8,9] which included comprehensive histology of 28 organs and tissues and a broad toxicological screening [8,9,10].…”

Section: Methodsmentioning

confidence: 99%

Detection of RNA viruses in sudden infant death (SID)

Bajanowski

Rolf

Jorch

et al. 2003

International Journal of Legal Medicine

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“…Virological investigations after culture and electron microscopical examination were negative in the swabs from the respiratory system in all 50 cases. Using PCR [23] cytomegalovirus could be deteted in the lung parenchyma in 1 casec of group C.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Reaction patterns in selected lymphatic tissues associated with sudden infant death (SID)

Bajanowski

Ortmann

Hernández

et al. 1997

International Journal of Legal Medicine

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In 50 cases of sudden infant death cervical, paratracheal and lung hilar lymph nodes, the thymus and the spleen were investigated by histology and immunohistochemistry (CD 20, 21, 45RO). The cases were divided into 3 groups based on autopsy findings including extensive histology: A --without pathological changes (N = 12), B --with minimal to intermediate inflammation (N = 23) and C --with severe inflammation (N = 15). In accordance with previous results the frequency of "pathological" lymph node changes, such as paracortical lymphoid hyperplasia and variegated hyperplasia of the pulp increased from group A to group C. The B-cell antigens reacted accordingly. A pronounced lymphodepletation of the thymus as a sign of a long lasting stimulation of the T-cell system was also observed increasingly from group A to C. In summary, in none of the cases results obtained were indicative of a defect of the T- or B-cell system. The results in group A seem to indicate that changes in the reaction pattern of the lymphoid tissues could be a more sensitive method of detection of early stages of inflammation than local histology.

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“…These molecular technologies have enabled laboratories to identify infectious agents that may not have been considered in the original diagnoses. Since, particularly, the human herpesvirus-6 (HHV-6), Epstein-Barr virus (EBV), and cytomegalovirus (CMV) have been previously detected in some cases of sudden death in infants [11][12][13][14][15], we decided to analyse the prevalence and viral loads of these viruses in tissues from SIDS patients. The high sensitivity of real-time PCR made us use this technique for that purpose.…”

Section: Introductionmentioning

confidence: 99%