Co-circulation of classic and novel astrovirus strains in patients with acute gastroenteritis in Germany

Jacobsen, Sonja; Höhne, Marina; Marques, Andreas Mas; Beslmüller, Klara; Bock, C.‐Thomas; Niendorf, Sandra

doi:10.1016/j.jinf.2018.02.006

Cited by 36 publications

(51 citation statements)

References 57 publications

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“…Yet, a statistically significant difference in novel HAstV prevalence according to the month of sample collection could be observed here, with January-April months being the ones with the highest detection rate. This seasonality is in agreement with the study of Jacobsen et al 30 , although the latter focused on both novel and classic HAstV. www.nature.com/scientificreports www.nature.com/scientificreports/ The novel HAstV positivity rate among control subjects was 4%.…”

Section: Discussionsupporting

confidence: 90%

A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea

Sabrià

Aregall

et al. 2020

Sci Rep

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Novel human astroviruses (HAstV) were discovered 10 years ago and have been associated with fatal cases of central nervous system infections. Their role in gastroenteritis is controversial, as they have been identified in symptomatic and asymptomatic subjects. The aim of the study was to investigate novel HAstV in a gastroenteritis case-control study including a pediatric population in Spain over a one-year period. We included stool samples from patients with gastroenteritis and negative results for viruses screened by routine diagnostics, and stool samples of control subjects who sought for a routine medical consultation. All samples were screened by real-time Rt-pcR assays for novel HAstV. An additional screening for rotavirus, norovirus GI, GII, sapovirus, classic HAstV and adenovirus was also performed for the control group. Overall, 23/363 stool samples from case patients (6.3%) and 8/199 stool samples from control patients (4%) were positive for ≥1 novel HAstV. MLB1 was predominant (64.5% of positives). Seasonality was observed for the case group (p = 0.015), but not the control group (p = 0.95). No difference was observed in the prevalence of novel HAstV between the case and control groups (OR 1.78, 95% CI 0.68-5.45; p = 0.30). Nevertheless, MLB genome copy numbers/ml of fecal suspension was significantly higher in the control group than in the case group (p = 0.008). In our study, we identified a lack of association between novel HAstV and gastroenteritis in the studied population, which could indicate a potential role of reservoir for children, especially given the higher viral load observed in the asymptomatic group for some of them.

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Section: Discussionsupporting

confidence: 90%

A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea

Sabrià

Aregall

et al. 2020

Sci Rep

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“…The highest incidence rate in this study has matched only with Mitab, 2013 study (6), this may be explained the high accuracy and sensitivity of the RT-qPCR assay in the detection of these viruses and this matched with what mentioned in other previous studies (14,25). In comparison to other global molecular based studies from Italy (11), Germany (20), lebanon (38), Egypt (4) and Iran (26). The prevalence of HAstV in this study is the highest, telling that this virus exists, flowing and contributing expressively to gastroenteritis in different Iraqi provinces.…”

Section: Figure 2 Melting Curve Of the Amplification Atrovirus Positsupporting

confidence: 86%

Molecular Detection of Astrovirus Using Reverse Transcriptase Quantitative Polymerase Chain Reaction Technique

Fadhil¹

2020

Iraqi J. Agric. Sci.

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This study was aimed to highlight the importance of the melt curve-quantitative reverse transcriptase PCR (RT-qPCR) analysis in the detection of astrovirus (AstV) from both negative and positive rotavirus and enterovirus (EVs) samples, and the effectiveness of the AstV infection on the vaccine immunogenicity in the vaccinated infected children. By RT-qPCR based Sybre green associated- melting curve assay, stool samples of 49 enterovirus suspected patients and of 39 rotavirus suspected patients were tested for AstV. Results of EVs group showed a 29 (59.2%) positive AstV contributed to 26 (89.5%) co-infection with Evs and 3 (10.3%) as a single infection in negative samples for Evs. Furthermore, AstV co-infection percentage is higher than the single infection. Moreover, the percentage of the Astrovirus among the vaccinated AFP-suspected cases was 53%, while the percentage of these viruses among the unvaccinated was 100%. Thus, MamAstrovirus- 1 MK948878 is the first local isolate recorded in the Genbank. In conclusion, the RT-qPCR based on SYBR Green showed the rapid and efficient detection of AstV with few copies number. This allow to be used for the diagnosis of AstV along with other gastroenteritis viruses in a multiplex assay to reduce processing time.

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“…The peak from December 2015 to April 2016 was much higher than the previous one. Two peaks of HAstV detection rate were observed in Germany, between February and May and between September and December (Jacobsen et al, 2018). In Korea, the pattern of seasonal distribution varied from one year to another (Kim et al, 2019).…”

Section: Discussionmentioning

confidence: 96%

Epidemiology and Genetic Characterization of Classical Human Astrovirus Infection in Shanghai, 2015–2016

Teng

Lin

et al. 2020

Front. Microbiol.

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Objective: Human astroviruses (HAstVs) are single-stranded RNA viruses of the Astroviridae family. Infection of classic HAstVs is one of the most common causes of acute viral gastroenteritis (infectious viral diarrhea). There is a lack of data on the prevalence and genetic characterization of classic HAstVs in acute viral gastroenteritis in the whole population. This study aimed to investigate the epidemiological trend, genotypes, viral co-infections, and viral loads of classic HAstVs in Shanghai, China, from January 2015 to December 2016. Methods: A total of 6,051 non-redundant stool samples were collected in outpatients with acute diarrhea in Shanghai from January 2015 to December 2016. One-step real-time RT-PCR was used for screening viral diarrhea, including rotavirus A, rotavirus B, rotavirus C, norovirus genotype I and II, classic human astroviruses, and sapovirus. Real-time PCR was used for screening human enteric adenoviruses. Conventional RT-PCR was used for the amplification of viral fragments for genotyping. PCR products were sequenced and used for the construction of phylogenetic trees. Results: The detection rate of classic HAstVs was 1.55% (94/6,051). The prevalence of HAstV infection displayed a typical winter/spring (December to March) seasonality and was highest in the 5-14 age group. Eighty-six samples were genotyped, which revealed HAstV-1 as the most prevalent genotype, followed by HAstV-5, HAstV-4, HAstV-2, HAstV-8, and HAstV-3. There was a dramatic rise in the prevalence of HAstV-4 from December 2015 to March 2016, and the viral loads of HAstV-4 were significantly higher than those of other genotypes. Among the mixed infection

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Co-circulation of classic and novel astrovirus strains in patients with acute gastroenteritis in Germany

Cited by 36 publications

References 57 publications

A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea

A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea

Molecular Detection of Astrovirus Using Reverse Transcriptase Quantitative Polymerase Chain Reaction Technique

Epidemiology and Genetic Characterization of Classical Human Astrovirus Infection in Shanghai, 2015–2016

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