Co-Circulation of Dengue Virus Type 3 Genotypes in Vientiane Capital, Lao PDR

Lao, Malayvanh; Caro, Valérie; Thiberge, Jean‐Michel; Bounmany, Phaitong; Vongpayloth, Khamsing; Buchy, Philippe; Duong, Veasna; Vanhlasy, Chansamone; Hospied, Jean-Marie; Thongsna, Manichanh; Choumlivong, Khamla; Vongkhamchanh, Phonesavanh; Oudavong, Bounleua; Brey, Paul T.; Grandadam, Marc

doi:10.1371/journal.pone.0115569

Cited by 34 publications

(88 citation statements)

References 14 publications

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“…The genotype II of DENV-3 is common in Southeast Asian countries and clusters within the viral strains from China, Myanmar, the Philippines, Bangladesh, Thailand, Cambodia, and Vietnam. The additional DENV-3 isolated in Vientiane in 2013 (LAOVTE13LN680428 and LAOVTE13LN680428) [7] belong to genotype III (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

“…PCR was performed by using cDNA derived from the DENV-2-, DENV-3-, or CHIKV-positive patients’ samples as a template and a primer pair for each target region to amplify the complete envelope (E) gene of DENV and E1 envelope glycoprotein gene of CHIKV. The following sets of specific primers for DENV-2 (Den2-911F 5′-TGACRG CTGTCGCTCCTTCA-3′, Den2-2444R 5′-CARCTCACAAYGCAACCACTATC-3′, 1485 bp), DENV-3 (Den3-815F 5′-GCCCTTAGGCACCCAGGGTT-3′, Den3-1752R 5′-CCCGCGAAAATGCTTGTGC-3′, Den3-1398F 5′-CGCAAGGAG TCACGGCTGAG-3′, Den3-2539R 5′-GCCTGCAATGGCTGTTGCC-3′, 1479 bp) [7], and CHIKV (Chik E1Fseq1 5′-GCTCCGCGTCCTTTACC-3′, Chik E1RSeq1 5′-ATGGCGACGCCCCCAAAGTC, 540 bp) were used for the PCR amplification. The PCR amplicons were directly sequenced using the BigDye® Terminator v3.1 cycle sequencing kit (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

“…DENV serotypes responsible for such infections in Laos were first confirmed in 1994, and a case involving co-infection with two DENV serotypes was reported [6]. Since then, larger epidemics caused by all four serotypes have occurred [7, 8]. DENV-1 has emerged in several provinces and caused sporadic clinical cases in different areas of Laos between 2010 and 2011 [8].…”

Section: Introductionmentioning

confidence: 99%

“…DENV-1 has emerged in several provinces and caused sporadic clinical cases in different areas of Laos between 2010 and 2011 [8]. The dominant circulating serotype subsequently switched from DENV-1 to DENV-3, and DENV-3 virus was the predominant DENV circulating in Laos at the end of June 2012 [7]. However, while some suspected cases of DENV infection were confirmed using laboratory detection, other cases of dengue infection were diagnosed based on clinical symptoms [9].…”

Section: Introductionmentioning

confidence: 99%

“…Infection of CHIKV has similar clinical presentations with DENV and co-circulates in overlapping geographic regions; hence, it can be underdiagnosed in areas where the DENV-endemic occurs [10]. Few studies of the molecular epidemiology of serotypes/or genotypes of DENV and CHIKV were reported in Laos [7, 8, 11]. …”

Section: Introductionmentioning

confidence: 99%

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Co-circulation of the dengue with chikungunya virus during the 2013 outbreak in the southern part of Lao PDR

et al. 2016

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BackgroundDuring the 2013 outbreak, 4638 infection cases and 32 deaths have been recorded in the southern part of Laos. In recent years, the chikungunya virus (CHIKV) emerged in the part of the country bordering Cambodia. Dengue virus (DENV) and CHIKV are transmitted by common mosquito vectors. Both diseases have similar clinical presentations; therefore, CHIKV infections might go undiagnosed in DENV-endemic areas. Thus, rapid detection and accurate diagnosis are crucial for differentiating between the two viruses (DENV and CHIKV). In this study, we demonstrated that CHIKV and two serotypes of DENV are circulating in Laos. In addition, we encountered patients that had been concurrently infected with multiple DENV serotypes or DENV and CHIKV.MethodsPlasma samples were collected from 40 patients with suspected DENV infections during an outbreak between July and August 2013. The reverse transcription polymerase chain reaction was performed to detect the four DENV serotypes and CHIKV using specific primers. Specifically, the complete envelope gene sequences of the viruses were sequenced and subjected to phylogenetic analysis.ResultsForty acute-phase plasma samples from patients with suspected dengue infections were tested for the presence of DENV viral RNA using molecular methods. Among the 40 samples, 14 samples were positive for DENV, 2 samples were positive for both viruses (DENV-2 and DENV-3), whereas DENV-1 and DENV-4 were not detected during the study period. We also encountered 10 samples that were positive for CHIKV. Of the 10 CHIKV-positive samples, 3 samples were co-infected by DENV-2, and 2 samples were co-infected by DENV-3. Phylogenetic analysis revealed that the 2013 dengue outbreak in Laos involved DENV-2 genotype Asian I and DENV-3 genotype II. Moreover, the Laotian CHIKV strains grouped together with those isolated during outbreaks on the Indian Ocean Islands within the East Central South African genotype.ConclusionsThese findings revealed that two serotypes (DENV-2 and DENV-3) and CHIKV were detected. Furthermore, infection of multiple DENV serotypes and CHIKV was also observed in the 2013 dengue outbreak. This is the first documented evidence of co-infection with CHIKV and one of two DENV serotypes.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%