Co-Circulation of the Rare CPV-2c with Unique Gln370Arg Substitution, New CPV-2b with Unique Thr440Ala Substitution, and New CPV-2a with High Prevalence and Variation in Heilongjiang Province, Northeast China

Geng, Yufei; Guo, Donghua; Li, Chunqiu; Wang, Enyu; Shan, Wei; Wang, Zhihui; Yao, Shuang; Zhao, Xiwen; Su, Mingjun; Wang, Xinyu; Wang, Jianfa; Wu, Rui; Feng, Li; Sun, Dongbo

doi:10.1371/journal.pone.0137288

Cited by 81 publications

(129 citation statements)

References 55 publications

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“…Whereas new CPV‐2a/2b are prevalent strains circulating in Asia (Yi, Tong, Cheng, Song, & Cheng, ), CPV‐2c has been sporadically detected in this continent. This variant has been detected in India in 2006 (Nandi, Chidri, Kumar, & Chauhan, ), in China from 2009 to 2015 (Geng et al., ; Wang et al., ; Zhang et al., ) and in Taiwan between 2014 and 2016 (Chiang, Wu, Chiou, Chang, & Lin, ). Our strain showed 100% nucleotide identity with complete VP2 sequence of 10 CPV strains detected in China in 2014 (Wang et al., ) and with partial VP2 sequence of 14 strains detected in China in years 2014‐2015 (Geng et al., ) and with partial VP2 sequence of 6 viruses circulating in Vientiane, Laos, in 2016 (Vannamahaxay et al., ).…”

Section: Resultsmentioning

confidence: 99%

“…Most recent Asian CPV strains exhibited four non‐synonymous nt changes that are responsible for aa substitutions Ala5Gly, Phe267Tyr, Tyr324Ile and Gln370Arg, and six synonymous nt changes (T135C, A207G, T101A, A732C, G1509A and A1659C) in the VP2 sequence (Table ). Change Tyr324Ile is predominant in recent Asian CPV strains (Yi et al., ; Geng et al., ; Zhao et al., ). In contrast, other changes have been observed only between 2013 and 2015 (Geng et al., ; Wang et al., ), and change Gln370Arg has been detected only in China in CPV strains from dogs and a strain from a giant panda (Guo et al., ).…”

Section: Resultsmentioning

confidence: 99%

“…Additional changes (Ser297Ala, Gly300Asp, Thr324Ile) in the VP2 sequence were also observed in strains detected in last decades (Ikeda et al., ; Jeoung, Ahn, & Kim, ; Truyen, ). Among these, a change at residue 324, first reported in 2006 (Zhou, Zeng, Zhang, & Li, ), may represent a common change in CPV strains circulating in the Asian continent (Geng et al., ). Recently, change Thr324Ile has been observed in European CPV‐2a strains (Cságola, Varga, Lőrincz, & Tuboly, ).…”

Section: Introductionmentioning

confidence: 99%

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Introduction of Asian canine parvovirus in Europe through dog importation

Mira

Purpari

Lorusso

et al. 2017

Transbound Emerg Dis

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Summary Canine parvovirus (CPV) is an important infectious agent of domestic and wild carnivores, responsible for severe and often fatal haemorrhagic gastroenteritis and leukopenia. This paper reports the genomic characterization of a CPV strain collected from a dog recently imported to Italy from Thailand. The virus was detected in all tissue samples collected. The whole genome encompassing the two open reading frames encoding for non‐structural (NS1/NS2) and structural (VP1/VP2) proteins was amplified and sequenced. On the basis of genetic analysis of the VP2 gene, the isolate was characterized as CPV‐2c, but it presented genetic signatures typical of Asian strains. Sequence analysis revealed the presence of amino acid changes never observed in European CPV‐2c strains (NS1: Ile60Val, Tyr544Phe, Glu545Val, Leu630Pro; VP2: Ala5Gly, Phe267Tyr, Tyr324Ile, Gln370Arg). By phylogenetic analysis of full‐length VP2 gene, the analysed strain clustered together with Asian viruses. Therefore, a possible introduction of the virus from Asia through the imported dog was suggested, thus confirming the important role of movement of dogs in the global spread of viruses. In addition, full‐length genome analysis could help better trace the spread of canine viruses through different continents.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Introduction of Asian canine parvovirus in Europe through dog importation

Mira

Purpari

Lorusso

et al. 2017

Transbound Emerg Dis

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“…9D). Whether the continuing capsid evolution of CPV in the domestic dog, coyote, and wolf populations (15,(42)(43)(44)(45)(46)(47) is associated with more efficient TfR binding remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

Single Mutations in the VP2 300 Loop Region of the Three-Fold Spike of the Carnivore Parvovirus Capsid Can Determine Host Range

Allison

Organtini

Zhang

et al. 2016

J Virol

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Sylvatic carnivores, such as raccoons, have recently been recognized as important hosts in the evolution of canine parvovirus (CPV), a pandemic pathogen of domestic dogs. Although viruses from raccoons do not efficiently bind the dog transferrin receptor (TfR) or infect dog cells, a single mutation changing an aspartic acid to a glycine at capsid (VP2) position 300 in the prototype raccoon CPV allows dog cell infection. Because VP2 position 300 exhibits extensive amino acid variation among the carnivore parvoviruses, we further investigated its role in determining host range by analyzing its diversity and evolution in nature and by creating a comprehensive set of VP2 position 300 mutants in infectious clones. Notably, some position 300 residues rendered CPV noninfectious for dog, but not cat or fox, cells. Changes of adjacent residues (residues 299 and 301) were also observed often after cell culture passage in different hosts, and some of the mutations mimicked changes seen in viruses recovered from natural infections of alternative hosts, suggesting that compensatory mutations were selected to accommodate the new residue at position 300. Analysis of the TfRs of carnivore hosts used in the experimental evolution studies demonstrated that their glycosylation patterns varied, including a glycan present only on the domestic dog TfR that dictates susceptibility to parvoviruses. Overall, there were significant differences in the abilities of viruses with alternative position 300 residues to bind TfRs and infect different carnivore hosts, demonstrating that the process of infection is highly host dependent and that VP2 position 300 is a key determinant of host range. IMPORTANCEAlthough the emergence and pandemic spread of canine parvovirus (CPV) are well documented, the carnivore hosts and evolutionary pathways involved in its emergence remain enigmatic. We recently demonstrated that a region in the capsid structure of CPV, centered around VP2 position 300, varies after transfer to alternative carnivore hosts and may allow infection of previously nonsusceptible hosts in vitro. Here we show that VP2 position 300 is the most variable residue in the parvovirus capsid in nature, suggesting that it is a critical determinant in the cross-species transfer of viruses between different carnivores due to its interactions with the transferrin receptor to mediate infection. To this end, we demonstrated that there are substantial differences in receptor binding and infectivity of various VP2 position 300 mutants for different carnivore species and that single mutations in this region can influence whether a host is susceptible or refractory to virus infection.T he emergence of viruses to cause epidemic or pandemic disease in new hosts is a continual threat to human and animal health. However, the viral and host mechanisms that control such events are often poorly understood. Although there are constant viral exposures occurring between many different species, most viruses do not infect or achieve sustained onward transmis...

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“…In Ecuador, CPV--2c was the most prevalent variant detected (54.7%) in rectal swabs of puppies (Aldaz et al 2013). Molecular epidemiology in stool samples of dogs with diarrhea from China showed CPV-2c in 14.74% of the samples (Geng et al 2015). To determine the CPV-2c circulation in USA, stool samples of vaccinated and unvaccinated dogs from 16 states were analyzed, showing that 25.92% of the samples contained CPV-2c (Hong et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Epidemiological, clinical and pathological features of canine parvovirus 2c infection in dogs from southern Brazil

et al. 2018

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Canine parvovirus type 2c (CPV-2c) emerged in Europe in the early 2000’s and rapidly spread out worldwide. Clinical and molecular data have demonstrated its circulation in Brazilian dogs, yet detailed descriptions of cases are still lacking. This article describes the epidemiological, clinical and pathological features of 24 cases of CPV-2c-associated disease in dogs submitted to veterinary clinics and laboratory diagnosis in southern Brazil (2014-2016). Most affected dogs presented signs/lesions suggestive of parvovirus enteritis: diarrhea, vomiting, hyperemia and hemorrhage of the serous membrane of the small intestine, diffuse segmental granulation, atrophy of the villi, necrosis and fusion of crypts, squamous metaplasia and epithelial syncytia. A number of cases presented features divergent from the classical presentations, including a wide variation in the color of feces (reddish and/or yellowish, light-brownish, orange-brown and brownish), involvement of adults (4/24) and vaccinated dogs (12/24), extensive involvement of the small intestine (8/20) and the presence of pulmonary edema (7/24) and convulsions (3/24). Feces and intestinal fragments submitted to PCR for the CPV-2 VP2 gene and to virus isolation in cell culture yielded positive results in 100% and 58.3% (14/24) of the cases, respectively. Nucleotide sequencing revealed a high nucleotide identity in VP2 (99.4 to 100%) and a consistent mutation at amino acid 426 (asparagine to glutamic acid), considered a signature of CPV-2c. These results confirm the involvement of CPV-2c in the described cases and demonstrate the importance of CPV-2c infection among Brazilian dogs, calling attention of veterinarians to correctly diagnose the disease, mainly considering the frequent atypical presentations.

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Co-Circulation of the Rare CPV-2c with Unique Gln370Arg Substitution, New CPV-2b with Unique Thr440Ala Substitution, and New CPV-2a with High Prevalence and Variation in Heilongjiang Province, Northeast China

Cited by 81 publications

References 55 publications

Introduction of Asian canine parvovirus in Europe through dog importation

Introduction of Asian canine parvovirus in Europe through dog importation

Single Mutations in the VP2 300 Loop Region of the Three-Fold Spike of the Carnivore Parvovirus Capsid Can Determine Host Range

Epidemiological, clinical and pathological features of canine parvovirus 2c infection in dogs from southern Brazil

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