Co-cultivation of Lactobacillus zeae and Veillonella criceti for the production of propionic acid

Abstract

In this work a defined co-culture of the lactic acid bacterium Lactobacillus zeae and the propionate producer Veillonella criceti has been studied in continuous stirred tank reactor (CSTR) and in a dialysis membrane reactor. It is the first time that this reactor type is used for a defined co-culture fermentation. This reactor allows high mixing rates and working with high cell densities, making it ideal for co-culture investigations. In CSTR experiments the co-culture showed over a broad concentration range an almost linear correlation in consumption and production rates to the supply with complex nutrients. In CSTR and dialysis cultures a strong growth stimulation of L. zeae by V. criceti was shown. In dialysis cultures very high propionate production rates (0.61 g L-1h-1) with final titers up to 28 g L-1 have been realized. This reactor allows an individual, intracellular investigation of the co-culture partners by omic-technologies to provide a better understanding of microbial communities.

Co-cultures with integrated in situ product removal for lactate-based propionic acid production

“…As more biomass is generated, the fouling on the membrane increases, and therefore, the resistance of the membrane stack increases, leading to higher voltages to uphold the current density. In comparison with previous studies, coculture coupled with electrodialysis has proven to enhance PA productivity, as maximum productivities reached were 0.33 g L −1 h −1 when using flour hydrolysate as substrate and 0.61 g L −1 h −1 while using glucose as a substrate in a dialysis reactor in continuous mode [10,27]. An overview of the generated experimental results can be found in Table 1.…”

Exosome Research and Co-culture Study

“…Cell behavior is often affected by material surfaces, and the influence of the filter material on the bottom of the upper chamber on behavior cannot be overlooked. Moreover, since it is necessary (A) Filter separation: for vertical connection, Transwell systems, 64,65) microfluidic systems, 51) bioreactor systems, 49,[66][67][68] and horizontal connection (this review, interactive co-culture plate, Ginreilab Inc., Japan). (B) Solid separation: cells in droplets, [69][70][71] microfluidic system.…”

Current advances in organic acid production from organic wastes by using microbial co‐cultivation systems

“…Some other co‐cultivation systems were also developed for propionic acid production. Lactobacillus zeae and Veillonella criceti have been considered as co‐cultivation systems for high propionic acid production . When a dialysis membrane was used in these co‐cultivation systems, 28 g.L ‐1 of propionic acid with a productivity of 0.61 g.(L·h) ‐1 was achieved.…”

“…Lactobacillus zeae and Veillonella criceti have been considered as co-cultivation systems for high propionic acid production. 16 When a dialysis membrane was used in these co-cultivation systems, 28 g.L -1 of propionic acid with a productivity of 0.61 g.(L·h) -1 was achieved. Notably, this reactor allows an individual, intracellular investigation of partners in this co-cultivation system by using omic technologies to elaborate the interaction between microbial communities better.…”