2023
DOI: 10.3390/jcm12031073
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Co-Culture of Cryopreserved Healthy Sertoli Cells with Testicular Tissue of Non-Obstructive Azoospermia (NOA) Patients in Culture Media Containing Follicle-Stimulating Hormone (FSH)/Testosterone Has No Advantage in Germ Cell Maturation

Abstract: Different cell culture conditions and techniques have been used to mature spermatogenic cells to increase the success of in vitro fertilization. Sertoli cells (SCs) are essential in maintaining spermatogenesis and FSH stimulation exerts its effect through direct or indirect actions on SCs. The effectiveness of FSH and testosterone added to the co-culture has been demonstrated in other studies to provide microenvironment conditions of the testicular niche and to contribute to the maturation and meiotic progress… Show more

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Cited by 2 publications
(2 citation statements)
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“…Identifying key proteins and miRNAs involved in spermatogenesis signaling pathways can provide diagnostic and regenerative targets for male infertility. The study of SC‐based exosomes and their role in intercellular communication has sparked interest in their potential therapeutic applications (Amiri et al., 2022, Aydos et al., 2023).…”
Section: Exosomes As Therapeutic Tools In Male Infertilitymentioning
confidence: 99%
“…Identifying key proteins and miRNAs involved in spermatogenesis signaling pathways can provide diagnostic and regenerative targets for male infertility. The study of SC‐based exosomes and their role in intercellular communication has sparked interest in their potential therapeutic applications (Amiri et al., 2022, Aydos et al., 2023).…”
Section: Exosomes As Therapeutic Tools In Male Infertilitymentioning
confidence: 99%
“…Afterwards, tissue samples obtained from patients with NOA were cultured in two distinct environments containing FSH and testosterone, or FSH, testosterone plus co-culture with healthy Sertoli cells. The results show that co-culture with FSH and testosterone was an effective treatment for germ cell maturation, while adding Sertoli cells from healthy individuals was insignificant compared to using the intact Sertoli cells retrieved from the patients’ own testes [ 15 ].…”
mentioning
confidence: 99%